Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MKZ05_RS12275 Genome accession   NZ_CP150255
Coordinates   2434212..2436536 (-) Length   774 a.a.
NCBI ID   WP_339160279.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0277     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2429212..2441536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ05_RS12260 (MKZ05_12260) dtd 2430861..2431304 (-) 444 WP_003216527.1 D-aminoacyl-tRNA deacylase -
  MKZ05_RS12265 (MKZ05_12265) - 2431323..2433533 (-) 2211 WP_012010732.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MKZ05_RS12270 (MKZ05_12270) - 2433676..2434188 (-) 513 WP_003216770.1 adenine phosphoribosyltransferase -
  MKZ05_RS12275 (MKZ05_12275) recJ 2434212..2436536 (-) 2325 WP_339160279.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MKZ05_RS12280 (MKZ05_12280) - 2436618..2436971 (-) 354 WP_251190258.1 lipopolysaccharide assembly LapA domain-containing protein -
  MKZ05_RS12285 (MKZ05_12285) - 2437070..2437315 (-) 246 Protein_2378 hypothetical protein -
  MKZ05_RS12290 (MKZ05_12290) secDF 2437495..2439723 (-) 2229 WP_003216337.1 protein translocase subunit SecDF -
  MKZ05_RS12295 (MKZ05_12295) - 2439765..2440061 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87350.07 Da        Isoelectric Point: 4.9218

>NTDB_id=968089 MKZ05_RS12275 WP_339160279.1 2434212..2436536(-) (recJ) [Bacillus sp. FSL M8-0277]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKELSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPTQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSMITEEAIQMVEGTGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEVGMLSPFGMQN
PKPFIMIEDVRLEEIRTIGANQNHIKMSLKDEDQLLDCVGFHQGQLKEEVVTGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKTISTLDPEKYVVVCFDEETKQEVKIPIHHVHAENVSTFDVEGKYVVFADVPADEHLLRQLLE
NQKPARVYTVFQRKEDHFMSSFPSRDQFKWFYGFLLKRGSFPLKEQGMELAKHRGWTKDTMIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=968089 MKZ05_RS12275 WP_339160279.1 2434212..2436536(-) (recJ) [Bacillus sp. FSL M8-0277]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTTGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGAATGAAAGAGGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGTT
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCTTTCCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACCGTCGATACAGGCATTGCAGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCGATTGTTCATCCTAAGCA
GCCAGGCTGTGAGTATCCCTTTAAAGAACTTGCCGGTGTAGGCGTAGCATTTAAAGTGGCGCATGCACTTCTTGGAAAGC
TGCCAACTCAGCTGTTAGATCTTGCGGCAATTGGGACAATAGCGGATCTTGTGCCGCTTCATGATGAGAATAGATGGCTC
GCTAAAAAAGGGCTTATGCAGCTTAGGCAGTCGAATAGACCAGGGCTGAAGGCGTTATTGAAAGAAGCAGGTGCTACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGACGAATTGAACAGGCCGATC
CAGCTGTTCATCTTCTCATGACAGAGGATAAGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAACTGAATAAAGAA
CGTCAAAAAATTGTGAGTATGATCACAGAAGAAGCCATTCAAATGGTGGAAGGGACAGGTGATGACCAGTCTGCAATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCGCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
TTAAGCAAATGCCGTGACATTCTGCCGCATTTTGGCGGGCACCCGATGGCTGCGGGTATGACACTTCGAGCGGATGATGT
TCCTTTACTAAGGGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAAGAAGACTTTATTCCGATTCAGCGGGTTG
ATGCTGTATGCAAGGTAGACGAGTTGACGGTTGAAGCAATTGAAGAAGTGGGGATGCTGTCACCATTTGGCATGCAGAAT
CCAAAGCCGTTCATCATGATTGAAGATGTGAGACTTGAAGAAATCCGAACAATTGGAGCAAATCAGAATCATATTAAAAT
GTCTCTGAAAGACGAAGACCAGCTGCTTGACTGTGTCGGTTTTCATCAAGGTCAATTAAAAGAAGAAGTTGTCACAGGTA
GCCGCATTTCCGTCATTGGTGAAATGTCCATCAATGAATGGAATAACCGGAAGAAACCACAGCTCATGCTAAAAGATGCC
CGAGTAGATGAGTGGCAGCTGTTTGATTTGCGCGGCAAAAAGGATTGGGAAAAGACAATCTCCACGCTTGACCCAGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGAAACAAAACAGGAAGTCAAAATCCCGATTCATCATGTCCATGCAGAAAATGTTT
CAACATTCGATGTAGAAGGCAAGTATGTTGTCTTTGCAGATGTTCCAGCAGATGAACACTTACTAAGGCAGCTGTTAGAA
AACCAAAAGCCAGCTAGAGTTTATACGGTTTTCCAAAGAAAAGAAGATCATTTCATGTCCTCTTTCCCTAGCCGTGATCA
ATTCAAATGGTTTTATGGATTCTTGTTAAAGCGAGGAAGTTTCCCTCTCAAGGAGCAAGGAATGGAGCTTGCGAAACATA
GAGGCTGGACAAAAGATACAATGATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(309-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.053

100

0.673

Multiple sequence alignment