Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NYE20_RS14095 Genome accession   NZ_CP150252
Coordinates   2649602..2650012 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus sp. FSL R5-0416     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2644602..2655012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE20_RS14065 (NYE20_14065) - 2644688..2644828 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NYE20_RS14070 (NYE20_14070) - 2645134..2645865 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  NYE20_RS14075 (NYE20_14075) cwlH 2646117..2646869 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NYE20_RS14080 (NYE20_14080) - 2647056..2647682 (+) 627 WP_021480068.1 TVP38/TMEM64 family protein -
  NYE20_RS14085 (NYE20_14085) gnd 2647702..2648595 (-) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NYE20_RS14090 (NYE20_14090) - 2648847..2649569 (+) 723 WP_049141193.1 hypothetical protein -
  NYE20_RS14095 (NYE20_14095) nucA/comI 2649602..2650012 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  NYE20_RS14100 (NYE20_14100) sigK 2650208..2650936 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  NYE20_RS14105 (NYE20_14105) - 2650936..2651034 (+) 99 WP_031600702.1 hypothetical protein -
  NYE20_RS14110 (NYE20_14110) - 2651031..2651205 (-) 175 Protein_2733 recombinase family protein -
  NYE20_RS14115 (NYE20_14115) - 2651163..2651341 (-) 179 Protein_2734 hypothetical protein -
  NYE20_RS14120 (NYE20_14120) psiE 2651886..2652302 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  NYE20_RS14125 (NYE20_14125) yrkQ 2652348..2653646 (-) 1299 WP_049141120.1 two-component system sensor histidine kinase YrkQ -
  NYE20_RS14130 (NYE20_14130) yrkP 2653633..2654328 (-) 696 WP_021480140.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=967894 NYE20_RS14095 WP_009967785.1 2649602..2650012(-) (nucA/comI) [Bacillus sp. FSL R5-0416]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=967894 NYE20_RS14095 WP_009967785.1 2649602..2650012(-) (nucA/comI) [Bacillus sp. FSL R5-0416]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment