Detailed information NTG0965560

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACKFA7_RS07640
• Genome accession: NZ_CP175553
• Coordinates: 1568426..1570837 (+)
• Length: 803 a.a.
• NCBI ID: WP_408856599.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain 4-9-2
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1563426..1575837

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKFA7_RS07615 (ACKFA7_07615)	-	1565068..1565943 (+)	876	WP_014417740.1	YicC/YloC family endoribonuclease	-
ACKFA7_RS07620 (ACKFA7_07620)	remA	1566020..1566289 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
ACKFA7_RS07625 (ACKFA7_07625)	gmk	1566297..1566911 (+)	615	WP_003154347.1	guanylate kinase	-
ACKFA7_RS07630 (ACKFA7_07630)	rpoZ	1566915..1567118 (+)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
ACKFA7_RS07635 (ACKFA7_07635)	coaBC	1567209..1568429 (+)	1221	WP_038457967.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ACKFA7_RS07640 (ACKFA7_07640)	priA	1568426..1570837 (+)	2412	WP_408856599.1	primosomal protein N'	Machinery gene
ACKFA7_RS07645 (ACKFA7_07645)	def	1570862..1571344 (+)	483	WP_029325903.1	peptide deformylase	-
ACKFA7_RS07650 (ACKFA7_07650)	fmt	1571349..1572302 (+)	954	WP_038457972.1	methionyl-tRNA formyltransferase	-
ACKFA7_RS07655 (ACKFA7_07655)	rsmB	1572289..1573632 (+)	1344	WP_038457974.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
ACKFA7_RS07660 (ACKFA7_07660)	rlmN	1573636..1574727 (+)	1092	WP_003154339.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
ACKFA7_RS07665 (ACKFA7_07665)	-	1574733..1575494 (+)	762	WP_038457977.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90848.83 Da
• Isoelectric Point: 8.8933

>NTDB_id=965560 ACKFA7_RS07640 WP_408856599.1 1568426..1570837(+) (priA) [Bacillus amyloliquefaciens strain 4-9-2]
MNVAEVIVDVSSKNIDRPFDYKIPDHLKGMIEVGMRVIVPFGPRKLQGFVTGLKDSSELNGGSMKEVEKLFDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIKQLFRNQKSLLYSDITDESILSSLQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQASKQLAVLTYFIGGGAKISAAELCKKTDSSSATLKTLLKKGL
LTESFEEVYRDPYQDRMFKKTEPLSLTEEQQAAFEPIKQAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGQEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSRELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAPK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQIQLKKILEHYKRDIAQKHVMISIDMNPY
KLM

Nucleotide

• Length: 2412 bp

>NTDB_id=965560 ACKFA7_RS07640 WP_408856599.1 1568426..1570837(+) (priA) [Bacillus amyloliquefaciens strain 4-9-2]
ATGAATGTAGCAGAAGTAATTGTCGATGTCAGCTCCAAAAACATTGACAGACCCTTTGATTACAAAATTCCCGATCATTT
AAAAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTTGGCCCGCGCAAGCTTCAAGGATTTGTGACCGGTTTGA
AAGATTCGTCTGAATTAAACGGCGGCTCAATGAAGGAAGTCGAGAAGCTTTTTGATTTGACGCCCGTTCTGACTGAGGAA
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATCACGGCGCTTCAGGCAATGCTGCCCGCCGC
ACTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAAACAGCTTTTCAGGA
ATCAAAAAAGTCTTCTGTACTCGGATATTACCGATGAAAGCATTCTTTCGTCACTTCAAAAATTCGTGCGCAAAGGAAGT
ATTGATGTCACATATAAAGTCGCCCAAAAAACGAATAAAAAAATGATCCGCATGATCGAAGCGGGGGCGGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAGCAGGCTTCTAAACAGCTCGCCGTGCTTACTTATTTTATCGGCGGGGGAG
CAAAGATTTCCGCGGCCGAGCTTTGCAAAAAGACCGATTCCAGTTCCGCGACGCTGAAAACACTTCTTAAAAAGGGGCTT
CTTACGGAAAGCTTTGAGGAGGTTTACCGCGATCCTTATCAGGACAGAATGTTCAAAAAAACAGAGCCGCTCTCTCTTAC
AGAAGAGCAGCAGGCCGCTTTTGAGCCGATTAAACAAGCTGTAGCCGATCATGTCCATCACGTCTTTCTGCTTCACGGCG
TCACGGGAAGCGGCAAAACGGAAATTTACCTGCAATCCATTGAAAAAGTGCTGGCAAAAGGACAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCGTTGACACCGCAAATGGTCAACCGTTTTAAAGGCCGCTTCGGCTCACAGGTTGCGGTCATGCACAG
CGGATTATCCACAGGTGAAAAATACGATGAATGGCGGAAGATTCAGCGCAAGGAAGTCCGGCTTGTCGTCGGAGCCAGAT
CGGCCATCTTTGCCCCGTTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCTTCCTATAAGCAGGAGGAA
ATGCCGCGGTATCATGCAAAGGACGTAGCCATCCGCCGGGCCGAGCATCACAGTTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAATCATTTGCGCGGGCGAAAAAAGGCGTTTATGAGCTGCTTCCGTTAAAACATCGCGTAAATCGCCAGC
TCATGCCTGACGTTTCTCTCGTTGATATGAGAGAAGAACTGAGAAACGGCAACAGATCAATGTTTTCAAGAGAGCTGATG
GAACAGCTGGAAGAGAAGCTTGCAAAAAAAGAGCAGGCCGTCCTTTTTCTGAATAAACGGGGGTATTCCTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCGCAATGTCCGCATTGCGACATTTCAATGACTTATCACCGCTACGGACAAAGGCTGA
AATGCCATTATTGCGGCCATGAGGAACCTGTCCCGGGAACATGCCCGGAATGCGGCAGCGAACATATCCGCTTTTTCGGC
ACGGGCACACAGCGTGTGGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACCAC
TTCAAGAAAAGGCGCCCATGAAAAACTGTTGTCAGCGTTCGGTGAAGGGAAGGCGGATATCCTGCTCGGTACGCAAATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCTGATTTC
AGGGCCGCGGAGAAGACATTCCAGCTGCTGACACAGGTAAGCGGACGGGCCGGGCGGCATGAAAAGCCGGGAACGGTGGT
CATTCAAACGTATACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCACGACTATGAAACTTTTTTTCAGCAGGAAA
TGGCGCACAGACGGGCCCAGTCATACCCGCCTTATTATTACGTGGCGCTGGTGACTGTTTCTCATGAAGAAGCGCCAAAA
GCCGCGGTCACCGCAGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGGCCTGATACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATCGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGATTC
AGTTGAAAAAGATACTGGAGCACTATAAACGCGACATTGCGCAAAAGCATGTAATGATTTCAATCGATATGAATCCGTAC
AAGCTGATGTAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	83.975	100	0.842