Detailed information NTG0960437

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: WKI55_RS13465
• Genome accession: NZ_CP149651
• Coordinates: 2644378..2646756 (-)
• Length: 792 a.a.
• NCBI ID: WP_017418224.1
• Uniprot ID: A0A411A8M2
• Organism: Bacillus velezensis strain MJ01
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2639378..2651756

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKI55_RS13430 (WKI55_13430)	-	2640451..2641178 (+)	728	Protein_2615	amino acid ABC transporter ATP-binding protein	-
WKI55_RS13435 (WKI55_13435)	-	2641199..2642026 (+)	828	WP_260654637.1	transporter substrate-binding domain-containing protein	-
WKI55_RS13440 (WKI55_13440)	-	2642083..2642733 (+)	651	WP_003152744.1	amino acid ABC transporter permease	-
WKI55_RS13445 (WKI55_13445)	-	2642752..2643408 (+)	657	WP_017418222.1	amino acid ABC transporter permease	-
WKI55_RS13450 (WKI55_13450)	-	2643444..2643575 (-)	132	WP_003152741.1	YrzQ family protein	-
WKI55_RS13455 (WKI55_13455)	-	2643597..2643788 (-)	192	WP_003152738.1	hypothetical protein	-
WKI55_RS13460 (WKI55_13460)	-	2643801..2644259 (-)	459	WP_017418223.1	PRC-barrel domain-containing protein	-
WKI55_RS13465 (WKI55_13465)	recD2	2644378..2646756 (-)	2379	WP_017418224.1	ATP-dependent RecD-like DNA helicase	Machinery gene
WKI55_RS13470 (WKI55_13470)	-	2646775..2647425 (-)	651	WP_007408203.1	tetratricopeptide repeat protein	-
WKI55_RS13475 (WKI55_13475)	mnmA	2647483..2648598 (-)	1116	WP_025649675.1	tRNA 2-thiouridine(34) synthase MnmA	-
WKI55_RS13480 (WKI55_13480)	-	2648635..2649773 (-)	1139	Protein_2625	cysteine desulfurase family protein	-
WKI55_RS13485 (WKI55_13485)	cymR	2649792..2650208 (-)	417	WP_017418225.1	cysteine metabolism transcriptional regulator CymR	-
WKI55_RS13490 (WKI55_13490)	-	2650403..2651671 (+)	1269	WP_025649676.1	replication-associated recombination protein A	-

Sequence

Protein

• Length: 792 a.a.
• Molecular weight: 88394.06 Da
• Isoelectric Point: 4.9490

>NTDB_id=960437 WKI55_RS13465 WP_017418224.1 2644378..2646756(-) (recD2) [Bacillus velezensis strain MJ01]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILTDASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDETFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQIKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide

• Length: 2379 bp

>NTDB_id=960437 WKI55_RS13465 WP_017418224.1 2644378..2646756(-) (recD2) [Bacillus velezensis strain MJ01]
GTGGACCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCATAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAAGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTACACATTTTACGGAAAAACCGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGGATCATTCACTATTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTCGGTGACGGCGCGCTCAATAAAATTCTGACGGATGCGTCGGTGCTTT
ACGATGTGCCGAAAATGTCGAAAAAGAAAGCGGATAAGCTTGCCGGAGAGCTGCAGCGGCATCAGGGGCTTGAGCAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCAATGAAAATATATCAGGCCTACGAAACGGAAACCCTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGCTGGGCGGAA
AAATGGGTCTGTCAGGAAACCACCCGGAGCGGATCAAAGCGGCAATCTTATATACGATTGAATCGACCTGTCTTTCAGAA
GGCCATACGTACATAGAGACAAAGCAGCTGATTATCAATACGCAAACGCTCCTGAATCAGTCAGCTGAAGGCGGCCGCGT
TACGGAGATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATTGAAGGAGAGCGCTGCTATT
TTCCGTCCTTGTTTTACGCCGAACAAAATGTCGCAAAACGCGTGCTCGACATTGCGGCTCAAACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTCGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTACTGACCGGAGGACCTGGGACGGGGAAAACGACCGTTATTAAAG
GAATCGTGGAATTATACAGTGAACTGCACGGAGTATCGCTTGACCCCGGTGATTATAAAAAAGACGAGACGTTCCCGGTG
CTGCTTGCCGCGCCGACGGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGGCTGCCGGCTGTTACGATTCACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTATAATGAAGATCAGCCGATTGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTCGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTCGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGACCTTTTGGCTTCTGAGGTGCTGCCGACCGTGCGTCT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCGCATGATATGAAAAAAGGACTGCTTCCAAAGG
GGCTGACCGCGCCGACAAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATTAAAGAAGTCGTGGATCAGGTCGTC
TCGAACGCGTTAAAAAAGGGGTATACCTCTAGGGATATTCAAGTGCTCGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAAGACATCATGAATCCGCCGAAAGAAAAAAGGCGGGAGCTGAAATTCGGTGATGTCGTCT
ACAGGACGGGCGATAAAATATTACAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAACGGCGATATCGGCGAAATCGTG
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCGTTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCTTACTGCTGTTCCATTCATAAGTCGCAGGGAAGCGAATTCCCGATTGTCG
TCCTTCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATAACAAGGGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTCAAAAACAATGATGCCACTGTCAGACAGACATCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATACG
ATGCGAACATCGGAATGGAAGGCATCACGCCTTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

• AAA domain (AAA_30) (341-548)

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (589-659)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (676-724)

• Helix-hairpin-helix containing domain (HHH_RecD2) (154-244)

3D structure

• AlphaFold DB: A0A411A8M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	88.748	99.874	0.886