Detailed information NTG0960366

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: WKI56_RS13075
• Genome accession: NZ_CP149650
• Coordinates: 2601979..2604357 (-)
• Length: 792 a.a.
• NCBI ID: WP_017418224.1
• Uniprot ID: A0A411A8M2
• Organism: Bacillus velezensis strain MJ02
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2596979..2609357

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKI56_RS13040 (WKI56_13040)	-	2598051..2598779 (+)	729	WP_014305489.1	amino acid ABC transporter ATP-binding protein	-
WKI56_RS13045 (WKI56_13045)	-	2598800..2599627 (+)	828	WP_017418221.1	transporter substrate-binding domain-containing protein	-
WKI56_RS13050 (WKI56_13050)	-	2599684..2600334 (+)	651	WP_003152744.1	amino acid ABC transporter permease	-
WKI56_RS13055 (WKI56_13055)	-	2600353..2601009 (+)	657	WP_017418222.1	amino acid ABC transporter permease	-
WKI56_RS13060 (WKI56_13060)	-	2601045..2601176 (-)	132	WP_003152741.1	YrzQ family protein	-
WKI56_RS13065 (WKI56_13065)	-	2601198..2601389 (-)	192	WP_003152738.1	hypothetical protein	-
WKI56_RS13070 (WKI56_13070)	-	2601402..2601860 (-)	459	WP_025649674.1	PRC-barrel domain-containing protein	-
WKI56_RS13075 (WKI56_13075)	recD2	2601979..2604357 (-)	2379	WP_017418224.1	ATP-dependent RecD-like DNA helicase	Machinery gene
WKI56_RS13080 (WKI56_13080)	-	2604376..2605026 (-)	651	WP_007408203.1	tetratricopeptide repeat protein	-
WKI56_RS13085 (WKI56_13085)	mnmA	2605084..2606199 (-)	1116	WP_012118077.1	tRNA 2-thiouridine(34) synthase MnmA	-
WKI56_RS13090 (WKI56_13090)	-	2606236..2607374 (-)	1139	Protein_2540	cysteine desulfurase family protein	-
WKI56_RS13095 (WKI56_13095)	cymR	2607393..2607809 (-)	417	WP_017418225.1	cysteine metabolism transcriptional regulator CymR	-
WKI56_RS13100 (WKI56_13100)	-	2608004..2609271 (+)	1268	Protein_2542	replication-associated recombination protein A	-

Sequence

Protein

• Length: 792 a.a.
• Molecular weight: 88394.06 Da
• Isoelectric Point: 4.9490

>NTDB_id=960366 WKI56_RS13075 WP_017418224.1 2601979..2604357(-) (recD2) [Bacillus velezensis strain MJ02]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILTDASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDETFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQIKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide

• Length: 2379 bp

>NTDB_id=960366 WKI56_RS13075 WP_017418224.1 2601979..2604357(-) (recD2) [Bacillus velezensis strain MJ02]
GTGGACCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCATAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAAGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTACACATTTTACGGAAAAACCGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGGATCATTCACTATTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTCGGTGACGGCGCGCTCAATAAAATTCTGACGGATGCGTCGGTGCTTT
ACGATGTGCCGAAAATGTCGAAAAAGAAAGCGGATAAGCTTGCCGGAGAGCTGCAGCGGCATCAGGGGCTTGAGCAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCAATGAAAATATATCAGGCCTACGAAACGGAAACCCTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGCTGGGCGGAA
AAATGGGTCTGTCAGGAAACCACCCGGAGCGGATCAAAGCGGCAATCTTATATACGATTGAATCGACCTGTCTTTCAGAA
GGCCATACGTACATAGAGACAAAGCAGCTGATTATCAATACGCAAACGCTCCTGAATCAGTCAGCTGAAGGCGGCCGCGT
TACGGAGATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATTGAAGGAGAGCGCTGCTATT
TTCCGTCCTTGTTTTACGCCGAACAAAATGTCGCAAAACGCGTGCTCGACATTGCGGCTCAAACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTCGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTACTGACCGGAGGACCTGGGACGGGGAAAACGACCGTTATTAAAG
GAATCGTGGAATTATACAGTGAACTGCACGGAGTATCGCTTGACCCCGGTGATTATAAAAAAGACGAGACGTTCCCGGTG
CTGCTTGCCGCGCCGACGGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGGCTGCCGGCTGTTACGATTCACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTATAATGAAGATCAGCCGATTGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTCGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTCGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGACCTTTTGGCTTCTGAGGTGCTGCCGACCGTGCGTCT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCGCATGATATGAAAAAAGGACTGCTTCCAAAGG
GGCTGACCGCGCCGACAAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATTAAAGAAGTCGTGGATCAGGTCGTC
TCGAACGCGTTAAAAAAGGGGTATACCTCTAGGGATATTCAAGTGCTCGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAAGACATCATGAATCCGCCGAAAGAAAAAAGGCGGGAGCTGAAATTCGGTGATGTCGTCT
ACAGGACGGGCGATAAAATATTACAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAACGGCGATATCGGCGAAATCGTG
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCGTTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCTTACTGCTGTTCCATTCATAAGTCGCAGGGAAGCGAATTCCCGATTGTCG
TCCTTCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATAACAAGGGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTCAAAAACAATGATGCCACCGTCAGACAGACGTCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATATG
ATGCGAACATCGGAATGGAAGGCATCACGCCGTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

• AAA domain (AAA_30) (341-548)

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (589-659)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (676-724)

• Helix-hairpin-helix containing domain (HHH_RecD2) (154-244)

3D structure

• AlphaFold DB: A0A411A8M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	88.748	99.874	0.886