Detailed information NTG0960315

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: WKI54_RS21360
• Genome accession: NZ_CP149648
• Coordinates: 4134360..4136738 (-)
• Length: 792 a.a.
• NCBI ID: WP_017418224.1
• Uniprot ID: A0A411A8M2
• Organism: Bacillus velezensis strain MJ04
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 4129360..4141738

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKI54_RS21325 (WKI54_21325)	-	4130433..4131160 (+)	728	Protein_4147	amino acid ABC transporter ATP-binding protein	-
WKI54_RS21330 (WKI54_21330)	-	4131181..4132008 (+)	828	WP_260654637.1	transporter substrate-binding domain-containing protein	-
WKI54_RS21335 (WKI54_21335)	-	4132065..4132715 (+)	651	WP_003152744.1	amino acid ABC transporter permease	-
WKI54_RS21340 (WKI54_21340)	-	4132734..4133390 (+)	657	WP_017418222.1	amino acid ABC transporter permease	-
WKI54_RS21345 (WKI54_21345)	-	4133426..4133557 (-)	132	WP_003152741.1	YrzQ family protein	-
WKI54_RS21350 (WKI54_21350)	-	4133579..4133770 (-)	192	WP_003152738.1	hypothetical protein	-
WKI54_RS21355 (WKI54_21355)	-	4133783..4134241 (-)	459	WP_017418223.1	PRC-barrel domain-containing protein	-
WKI54_RS21360 (WKI54_21360)	recD2	4134360..4136738 (-)	2379	WP_017418224.1	ATP-dependent RecD-like DNA helicase	Machinery gene
WKI54_RS21365 (WKI54_21365)	-	4136757..4137407 (-)	651	WP_007408203.1	tetratricopeptide repeat protein	-
WKI54_RS21370 (WKI54_21370)	mnmA	4137465..4138580 (-)	1116	WP_025649675.1	tRNA 2-thiouridine(34) synthase MnmA	-
WKI54_RS21375 (WKI54_21375)	-	4138617..4139756 (-)	1140	WP_007612815.1	cysteine desulfurase family protein	-
WKI54_RS21380 (WKI54_21380)	cymR	4139775..4140191 (-)	417	WP_017418225.1	cysteine metabolism transcriptional regulator CymR	-
WKI54_RS21385 (WKI54_21385)	-	4140386..4141654 (+)	1269	WP_025649676.1	replication-associated recombination protein A	-

Sequence

Protein

• Length: 792 a.a.
• Molecular weight: 88394.06 Da
• Isoelectric Point: 4.9490

>NTDB_id=960315 WKI54_RS21360 WP_017418224.1 4134360..4136738(-) (recD2) [Bacillus velezensis strain MJ04]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILTDASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDETFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQIKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide

• Length: 2379 bp

>NTDB_id=960315 WKI54_RS21360 WP_017418224.1 4134360..4136738(-) (recD2) [Bacillus velezensis strain MJ04]
GTGGACCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCATAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAAGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTACACATTTTACGGAAAAACCGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGGATCATTCACTATTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTCGGTGACGGCGCGCTCAATAAAATTCTGACGGATGCGTCGGTGCTTT
ACGATGTGCCGAAAATGTCGAAAAAGAAAGCGGATAAGCTTGCCGGAGAGCTGCAGCGGCATCAGGGGCTTGAGCAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCAATGAAAATATATCAGGCCTACGAAACGGAAACCCTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGCTGGGCGGAA
AAATGGGTCTGTCAGGAAACCACCCGGAGCGGATCAAAGCGGCAATCTTATATACGATTGAATCGACCTGTCTTTCAGAA
GGCCATACGTACATAGAGACAAAGCAGCTGATTATCAATACGCAAACGCTCCTGAATCAGTCAGCTGAAGGCGGCCGCGT
TACGGAGATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATTGAAGGAGAGCGCTGCTATT
TTCCGTCCTTGTTTTACGCCGAACAAAATGTCGCAAAACGCGTGCTCGACATTGCGGCTCAAACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTCGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTACTGACCGGAGGACCTGGGACGGGGAAAACGACCGTTATTAAAG
GAATCGTGGAATTATACAGTGAACTGCACGGAGTATCGCTTGACCCCGGTGATTATAAAAAAGACGAGACGTTCCCGGTG
CTGCTTGCCGCGCCGACGGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGGCTGCCGGCTGTTACGATTCACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTATAATGAAGATCAGCCGATTGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTCGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTCGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGACCTTTTGGCTTCTGAGGTGCTGCCGACCGTGCGTCT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCGCATGATATGAAAAAAGGACTGCTTCCAAAGG
GGCTGACCGCGCCGACAAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATTAAAGAAGTCGTGGATCAGGTCGTC
TCGAACGCGTTAAAAAAGGGGTATACCTCTAGGGATATTCAAGTGCTCGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAAGACATCATGAATCCGCCGAAAGAAAAAAGGCGGGAGCTGAAATTCGGTGATGTCGTCT
ACAGGACGGGCGATAAAATATTACAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAACGGCGATATCGGCGAAATCGTG
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCGTTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCTTACTGCTGTTCCATTCATAAGTCGCAGGGAAGCGAATTCCCGATTGTCG
TCCTTCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATAACAAGGGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTCAAAAACAATGATGCCACTGTCAGACAGACATCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATACG
ATGCGAACATCGGAATGGAAGGCATCACGCCTTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

• AAA domain (AAA_30) (341-548)

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (589-659)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (676-724)

• Helix-hairpin-helix containing domain (HHH_RecD2) (154-244)

3D structure

• AlphaFold DB: A0A411A8M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	88.748	99.874	0.886