Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACFELG_RS26310 Genome accession   NZ_CP172848
Coordinates   5652555..5653151 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain 238628E_ID004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5647555..5658151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELG_RS26290 (ACFELG_26290) aroK 5648686..5649204 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACFELG_RS26295 (ACFELG_26295) pilQ 5649216..5651360 (-) 2145 WP_012614629.1 type 4a pilus secretin PilQ Machinery gene
  ACFELG_RS26300 (ACFELG_26300) comL/pilP 5651414..5651938 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ACFELG_RS26305 (ACFELG_26305) pilO 5651935..5652558 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACFELG_RS26310 (ACFELG_26310) pilN 5652555..5653151 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACFELG_RS26315 (ACFELG_26315) pilM 5653151..5654215 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACFELG_RS26320 (ACFELG_26320) - 5654400..5656871 (+) 2472 WP_023090127.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=954560 ACFELG_RS26310 WP_003095839.1 5652555..5653151(-) (pilN) [Pseudomonas aeruginosa strain 238628E_ID004]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=954560 ACFELG_RS26310 WP_003095839.1 5652555..5653151(-) (pilN) [Pseudomonas aeruginosa strain 238628E_ID004]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTACTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369