Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   WBM82_RS13230 Genome accession   NZ_CP147494
Coordinates   2496696..2497733 (-) Length   345 a.a.
NCBI ID   WP_029726718.1    Uniprot ID   -
Organism   Bacillus subtilis strain YT1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2491696..2502733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WBM82_RS13180 (WBM82_13225) tasA 2491793..2492578 (-) 786 WP_014664586.1 biofilm matrix protein TasA -
  WBM82_RS13185 (WBM82_13230) sipW 2492642..2493214 (-) 573 WP_072692741.1 signal peptidase I -
  WBM82_RS13190 (WBM82_13235) tapA 2493198..2493959 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  WBM82_RS13195 (WBM82_13240) yqzG 2494231..2494557 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  WBM82_RS13200 (WBM82_13245) spoIIT 2494599..2494778 (-) 180 WP_029726723.1 YqzE family protein -
  WBM82_RS13205 (WBM82_13250) comGG 2494850..2495224 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  WBM82_RS13210 (WBM82_13255) comGF 2495225..2495608 (-) 384 WP_029726721.1 ComG operon protein ComGF Machinery gene
  WBM82_RS13215 (WBM82_13260) comGE 2495634..2495981 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  WBM82_RS13220 (WBM82_13265) comGD 2495965..2496396 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  WBM82_RS13225 (WBM82_13270) comGC 2496386..2496682 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  WBM82_RS13230 (WBM82_13275) comGB 2496696..2497733 (-) 1038 WP_029726718.1 comG operon protein ComGB Machinery gene
  WBM82_RS13235 (WBM82_13280) comGA 2497720..2498790 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  WBM82_RS13240 (WBM82_13285) - 2499003..2499200 (-) 198 WP_029726717.1 hypothetical protein -
  WBM82_RS13245 (WBM82_13290) corA 2499202..2500155 (-) 954 WP_004399136.1 magnesium transporter CorA -
  WBM82_RS13250 (WBM82_13295) yqhB 2500298..2501626 (+) 1329 WP_029726716.1 hemolysin family protein -
  WBM82_RS13255 (WBM82_13300) rsbRD 2501679..2502515 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39702.10 Da        Isoelectric Point: 9.6257

>NTDB_id=946430 WBM82_RS13230 WP_029726718.1 2496696..2497733(-) (comGB) [Bacillus subtilis strain YT1]
MKKIRKVWSLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDILFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=946430 WBM82_RS13230 WP_029726718.1 2496696..2497733(-) (comGB) [Bacillus subtilis strain YT1]
ATGAAAAAGATTAGAAAAGTCTGGTCGTTAAAGGATCAAGCGAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACGCTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATACTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTCAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.381

93.623

0.93