Detailed information NTG0945328

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACFXAC_RS07745
• Genome accession: NZ_CP170720
• Coordinates: 1555313..1557724 (+)
• Length: 803 a.a.
• NCBI ID: WP_053573428.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JP5006
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1550313..1562724

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFXAC_RS07720 (ACFXAC_07720)	-	1551955..1552830 (+)	876	WP_053573462.1	YicC/YloC family endoribonuclease	-
ACFXAC_RS07725 (ACFXAC_07725)	remA	1552907..1553176 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
ACFXAC_RS07730 (ACFXAC_07730)	gmk	1553184..1553798 (+)	615	WP_003154347.1	guanylate kinase	-
ACFXAC_RS07735 (ACFXAC_07735)	rpoZ	1553802..1554005 (+)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
ACFXAC_RS07740 (ACFXAC_07740)	coaBC	1554096..1555316 (+)	1221	WP_053573427.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ACFXAC_RS07745 (ACFXAC_07745)	priA	1555313..1557724 (+)	2412	WP_053573428.1	primosomal protein N'	Machinery gene
ACFXAC_RS07750 (ACFXAC_07750)	def	1557749..1558231 (+)	483	WP_014417743.1	peptide deformylase	-
ACFXAC_RS07755 (ACFXAC_07755)	fmt	1558236..1559189 (+)	954	WP_014417744.1	methionyl-tRNA formyltransferase	-
ACFXAC_RS07760 (ACFXAC_07760)	rsmB	1559176..1560519 (+)	1344	WP_014417745.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
ACFXAC_RS07765 (ACFXAC_07765)	rlmN	1560523..1561614 (+)	1092	WP_053573429.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
ACFXAC_RS07770 (ACFXAC_07770)	-	1561620..1562381 (+)	762	WP_007409750.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90819.85 Da
• Isoelectric Point: 8.8944

>NTDB_id=945328 ACFXAC_RS07745 WP_053573428.1 1555313..1557724(+) (priA) [Bacillus amyloliquefaciens strain JP5006]
MNVAEVIVDVSSKNIDRPFDYKIPDHLKGMIEVGMRVIVPFGPRKLQGFVTGLKDSSELNGGSMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIKQLFRNQKSLLYSDITDESILSSLQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQASKQLAVLTYFIGGGAKISAAELCKKTDSSSATLKTLLKKGL
LTESFEEVYRDPYQDRMFKKTEPLSLTEEQRAAFEPIKQAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGQEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSRELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQIQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=945328 ACFXAC_RS07745 WP_053573428.1 1555313..1557724(+) (priA) [Bacillus amyloliquefaciens strain JP5006]
ATGAATGTAGCAGAAGTCATTGTCGATGTCAGCTCCAAAAACATTGACAGACCCTTTGATTACAAAATTCCCGATCATTT
AAAAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTTGGCCCGCGCAAGCTTCAAGGATTTGTGACCGGTTTGA
AAGATTCGTCTGAATTAAACGGCGGCTCAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACGCCCGTTCTGACTGAGGAA
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATTACGGCGCTTCAGGCAATGCTGCCCGCCGC
ACTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAAACAGCTTTTCAGGA
ATCAAAAAAGTCTTCTGTACTCGGATATTACCGATGAAAGCATTCTTTCGTCACTTCAAAAATTCGTGCGCAAAGGAAGC
ATTGATGTCACATATAAAGTCGCCCAAAAAACGAATAAAAAAATGATCCGCATGATCGAAGCGGGGGCAGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAGCAGGCTTCTAAACAGCTCGCCGTGCTTACTTATTTTATCGGCGGGGGAG
CAAAGATTTCCGCGGCCGAGCTTTGCAAAAAGACCGATTCCAGTTCCGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGAAAGCTTTGAGGAGGTTTACCGCGATCCCTATCAGGACAGAATGTTCAAAAAAACAGAGCCGCTCTCTCTTAC
AGAAGAGCAGCGGGCTGCTTTTGAGCCGATTAAACAAGCCGTAGCCGACCATGTCCATCACGTCTTTCTGCTTCACGGCG
TCACTGGAAGCGGCAAAACGGAAATTTACCTGCAATCCATTGAAAAAGTGCTGGCAAAAGGACAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCGTTGACACCGCAAATGGTCAACCGTTTTAAAGGCCGCTTCGGCTCACAGGTTGCGGTCATGCACAG
CGGATTATCCACAGGTGAAAAATACGATGAATGGCGGAAGATTCAGCGCAAGGAAGTCCGGCTTGTTGTCGGAGCCAGAT
CGGCCATCTTTGCCCCGTTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCTTCCTATAAGCAGGAGGAA
ATGCCGCGGTATCATGCAAAGGACGTAGCCATCCGCCGGGCCGAGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAATCATTTGCGCGGGCGAAAAAAGGCGTTTATGAGCTGCTACCGTTAAAACATCGCGTAAATCGCCAGC
TCATGCCTGACGTTTCTCTCGTTGATATGAGAGAAGAACTGAGAAACGGCAACAGATCAATGTTTTCAAGAGAGCTGATG
GAACAGCTGGAAGAGAAGCTTGCAAAAAAAGAGCAGGCTGTCCTTTTCCTGAATAAACGGGGGTATTCCTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCGCAATGTCCGCATTGCGACATTTCAATGACTTATCACCGCTACGGACAAAGGCTGA
AATGCCATTACTGCGGCCATGAGGAACCTGTCCCGGGAACATGCCCGGAATGCGGCAGCGAACATATCCGCTTTTTCGGC
ACAGGCACGCAGCGTGTGGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACCAC
TTCAAGAAAAGGCGCCCATGAAAAACTGTTGTCAGCGTTCGGTGAAGGGAAGGCGGATATCCTGCTCGGTACGCAAATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCTGATTTC
AGGGCTGCGGAGAAGACATTCCAGCTGCTGACGCAGGTAAGCGGACGGGCCGGGCGGCATGAAAAGCCGGGAACGGTGGT
CATTCAAACGTATACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCACGACTATGAAACTTTTTTTCAGCAGGAAA
TGGCGCACAGACGGGCCCAGTCATACCCGCCTTATTATTACGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCGGCAAAA
GCCGCGGTCACCGCAGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGGCCTGATACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATCGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGATTC
AGTTGAAAAAGATACTGGAGCACTATAAACGCGACATTGCGCAAAAGCATGTAATGATTTCAATCGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	84.472	100	0.847