Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   WAA16_RS12735 Genome accession   NZ_CP146984
Coordinates   2479055..2481379 (-) Length   774 a.a.
NCBI ID   WP_260336214.1    Uniprot ID   -
Organism   Bacillus pumilus strain S9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2474055..2486379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAA16_RS12720 (WAA16_12720) dtd 2475704..2476147 (-) 444 WP_260336213.1 D-aminoacyl-tRNA deacylase -
  WAA16_RS12725 (WAA16_12725) - 2476166..2478376 (-) 2211 WP_060596867.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  WAA16_RS12730 (WAA16_12730) - 2478519..2479031 (-) 513 WP_034661543.1 adenine phosphoribosyltransferase -
  WAA16_RS12735 (WAA16_12735) recJ 2479055..2481379 (-) 2325 WP_260336214.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  WAA16_RS12740 (WAA16_12740) - 2481461..2481814 (-) 354 WP_060596869.1 lipopolysaccharide assembly LapA domain-containing protein -
  WAA16_RS12745 (WAA16_12745) - 2481916..2482404 (-) 489 WP_066030599.1 cation:proton antiporter regulatory subunit -
  WAA16_RS12750 (WAA16_12750) secDF 2482592..2484820 (-) 2229 WP_060596871.1 protein translocase subunit SecDF -
  WAA16_RS12755 (WAA16_12755) - 2484862..2485158 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87498.15 Da        Isoelectric Point: 4.9619

>NTDB_id=945312 WAA16_RS12735 WP_260336214.1 2479055..2481379(-) (recJ) [Bacillus pumilus strain S9]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPAQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDRFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDFLPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEVGMLSPFGMQN
PKPFIMIEDARLEEIRTIGANQNHIKMSLKDEDKLLDCVGFHQGQLKEEIVTGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKTISTLDPEKYVVVCFDEETKQEVNIPIYHVHAENVSTFDVEGKYIVFADVPADEHLLRRLLE
NQKPARVYTVFQRKEDHFMSSFPSRDQFKWFYGFLLKRGSFPLKEQGMELAKHRGWTKETIIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=945312 WAA16_RS12735 WP_260336214.1 2479055..2481379(-) (recJ) [Bacillus pumilus strain S9]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGGATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGCG
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCTTTTCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACGGTCGATACAGGCATTGCAGCTGTAAATGAAGCTCGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCGATTGTCCATCCTAAGCA
GCCGGGCTGTGAGTATCCTTTTAAAGAACTTGCCGGTGTAGGCGTAGCGTTTAAAGTGGCACATGCACTTCTTGGGAAAC
TGCCAGCTCAGCTGTTAGATCTTGCGGCAATTGGGACAATAGCGGATCTTGTGCCGCTTCATGATGAGAATAGATGGCTC
GCCAAAAAAGGGCTTGTGCAGCTTCGACAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCTACTCT
TGAAGAAGCAAATGAAGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGGCGAATTGAGCAGGCAGATC
CAGCTGTTCATCTTCTCATGACAGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAAGAACTCAATAAAGAA
CGTCAAAAAATTGTGAGTACAATCACAGAAGAAGCCATTCAAATGGTTGAAGAGACAGGTGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATAGATTTTCACGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCGCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
TTAAGCAAATGCCGTGACTTTCTGCCGCATTTCGGCGGGCACCCGATGGCTGCGGGTATGACACTTCGAGCGGATGATGT
TCCTTTACTAAGAGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAAGAAGATTTTATTCCGATTCAGCGGGTCG
ATGCCGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGTGGGGATGCTGTCACCATTTGGCATGCAGAAT
CCAAAGCCGTTCATCATGATTGAAGATGCGAGGCTTGAAGAGATCCGAACGATTGGGGCAAATCAGAATCATATTAAAAT
GTCTCTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGTTTTCATCAAGGTCAATTAAAAGAAGAAATTGTCACAGGTA
GCCGCATTTCCGTCATTGGTGAAATGTCCATCAATGAATGGAATAACCGGAAGAAACCTCAGCTTATGCTAAAAGATGCC
CGAGTAGATGAATGGCAGCTGTTTGATTTGCGCGGCAAAAAGGATTGGGAAAAGACAATCTCCACGCTTGATCCAGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGAAACAAAACAGGAAGTCAATATCCCAATTTATCATGTCCATGCAGAAAATGTTT
CAACGTTCGATGTAGAGGGCAAGTATATCGTCTTTGCAGATGTTCCTGCAGATGAGCATTTGCTAAGGCGACTTTTAGAA
AACCAAAAGCCAGCTAGAGTTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCTTCTTTCCCTAGCCGTGATCA
ATTCAAATGGTTTTATGGATTCTTGTTAAAACGAGGAAGTTTCCCCCTCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGAAACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(309-437)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.568

100

0.678