Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   WAA16_RS05130 Genome accession   NZ_CP146984
Coordinates   1046874..1050383 (+) Length   1169 a.a.
NCBI ID   WP_060595950.1    Uniprot ID   -
Organism   Bacillus pumilus strain S9     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1041874..1055383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAA16_RS05100 (WAA16_05100) lepB 1042382..1042894 (+) 513 WP_034663490.1 signal peptidase I -
  WAA16_RS05105 (WAA16_05105) - 1042929..1044074 (-) 1146 WP_060595948.1 AbrB family transcriptional regulator -
  WAA16_RS05110 (WAA16_05110) - 1044341..1044763 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  WAA16_RS05115 (WAA16_05115) - 1044756..1045595 (+) 840 WP_060595949.1 M56 family metallopeptidase -
  WAA16_RS05120 (WAA16_05120) - 1045653..1046063 (+) 411 WP_034663477.1 DoxX family protein -
  WAA16_RS05125 (WAA16_05125) - 1046260..1046706 (-) 447 WP_034663474.1 ferritin-like domain-containing protein -
  WAA16_RS05130 (WAA16_05130) addB 1046874..1050383 (+) 3510 WP_060595950.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  WAA16_RS05135 (WAA16_05135) addA 1050361..1054065 (+) 3705 WP_106031476.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  WAA16_RS05140 (WAA16_05140) sbcD 1054092..1055267 (+) 1176 WP_060595952.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134714.74 Da        Isoelectric Point: 5.3106

>NTDB_id=945284 WAA16_RS05130 WP_060595950.1 1046874..1050383(+) (addB) [Bacillus pumilus strain S9]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISMSSMYSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPSSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYQLARECGADISENVLERNKRHIYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDCDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLYQLQKRLKKAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIVGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSRHLYLSYSAADAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLDDEEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPHRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSKHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDDIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=945284 WAA16_RS05130 WP_060595950.1 1046874..1050383(+) (addB) [Bacillus pumilus strain S9]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGAATGATCAGGGCCAAAGTATTTAGTTTCACACGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGAAAAGTT
TAAAGTGTTCAAAAAAGCGAGTGATAAGCCGGGATTTGTGGAGCAAATTGAAAAGACGATGGCTGAATTCAAAAGGTATT
GTATGTTGCCGGAGGATATTGAAAAAATTTCAATGTCGAGCATGTATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACACTTCTCACGCAGCAAATTCCTTCATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCCTTCTGCATGCAGCAAAAATAACTGCTGCTTTCACAGTGGATCGCTCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGGAAAACGTATTTCCAGCTCTATCAGCTTGCTAG
AGAGTGCGGAGCTGACATTTCTGAGAACGTTTTGGAAAGAAATAAACGGCACATTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGAGACCAGTGCAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGTGTAGCCAGAGATATACTTGATTTAGTGAGAGAAAAAGGACTTAGATTGAGAGATATCTCGAT
TGTTGCACGACATGTAGACGACTATAAAGATACATTAAAAGAGGTTTTTCGAGATTGCGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAGGGAAACTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTTGAACTCGCTAAGAACAAAGCGAGAGAGCAGGCAGA
TCAGCTCGAAAACTATTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGCT
TCCAATCATTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTCAAAGAATGG
ATCGTGCCCCCGCTTTACCAGTTGCAGAAAAGGCTCAAAAAAGCGCAAAAGGTAAGAGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTAGATTAGCTGAAG
CAATGCAGCACGGACAAGTATGGGATGCGGTTATTCAATTAATGGATGAATTTGTTGAAATGCTAGGTGAAGAAGAGCTC
TCATTTCCTTTATTCCAACAAATGATGGATACAGGATTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGACCA
AGTATTTATTGGAAGTATGGATTTATCGAGAATGTATCAAGTGAAGTGTACGTTTATCGTTGGTGTAAATGATGGCGTTA
TTCCCGCACGCCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGTGAATGGTTAAAACGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCGAGTCCATCACGTCATTTGTATCT
ATCCTATTCGGCTGCTGATGCAGAAGGACGTTCTCTTTTGCCTTCGACACTTATCAAGTATTGCCAAGAGCTTATGCCAA
ATCACCAGCAGGCTCTTTATGTGTTAGATCCAGAACAACTGGATGACGAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTATCGTATACCGTTTCACAGTTGCAGCAATGGCTCAATCAATATCCCATTAGTGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTCATCGGGATGTATCAAAAAACATCATGTCAAGTTTGTTTTTTACAAATCGAGCGAAGC
CATTAAAGCCTAATGTCACAAAAGAGCTTTATGGTGATCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCATGGGTTAAAACTAAAGGATCGACAATTTTACAAATTGGAAGCACCTGATATCGG
CCAGTTGTTCCACTCGGCTTTAAAACATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTCACTTATTCAAAACATGCGATTGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAAGTGAG
TGGATTTTCTCCAGTAGGGCTAGAGTTAGGTTTTGGGGGGCAAGGGCCATTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGCTCTTGAGAATAGTC
GACTACAAATCCAGTGAGAAAGGTCTTGACTTAGCAGAAGTATACTATGGGCTGGCCTTGCAAATGCTCACTTACTTAGA
CCTGACCATTACGTATTCGAAAGAGTGGCTCGGGGTTGAAGCGACGCCTGCTGGCATTTTATATTTCCATATCCATGATC
CGCTCATTCAAGCACCTATTCCGCTTGCGGAGGATGACATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAGGGTTTA
TTGCTTGAAGATGTAGAAGCAGTAAAACTCATGGATCAAACGCTTGATTCAGGTAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGACTCTGCAGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTGAAGGATCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAATTCGATGAATCAATAGAGGATAATGATTTTAGGGTGCTCACCTCTGA
GAAAGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.236

99.316

0.658