Detailed information NTG0945130

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACFW98_RS01735
• Genome accession: NZ_CP170719
• Coordinates: 367753..370164 (+)
• Length: 803 a.a.
• NCBI ID: WP_015417447.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JP5008
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 362753..375164

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFW98_RS01710 (ACFW98_01710)	-	364389..365264 (+)	876	WP_061890949.1	YicC/YloC family endoribonuclease	-
ACFW98_RS01715 (ACFW98_01715)	remA	365341..365610 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
ACFW98_RS01720 (ACFW98_01720)	gmk	365618..366232 (+)	615	WP_003154347.1	guanylate kinase	-
ACFW98_RS01725 (ACFW98_01725)	rpoZ	366236..366439 (+)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
ACFW98_RS01730 (ACFW98_01730)	coaBC	366530..367756 (+)	1227	WP_324635857.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ACFW98_RS01735 (ACFW98_01735)	priA	367753..370164 (+)	2412	WP_015417447.1	primosomal protein N'	Machinery gene
ACFW98_RS01740 (ACFW98_01740)	def	370189..370671 (+)	483	WP_033574585.1	peptide deformylase	-
ACFW98_RS01745 (ACFW98_01745)	fmt	370676..371629 (+)	954	WP_076424120.1	methionyl-tRNA formyltransferase	-
ACFW98_RS01750 (ACFW98_01750)	rsmB	371616..372959 (+)	1344	WP_395096738.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
ACFW98_RS01755 (ACFW98_01755)	rlmN	372963..374054 (+)	1092	WP_014417746.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
ACFW98_RS01760 (ACFW98_01760)	-	374060..374821 (+)	762	WP_007409750.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90790.81 Da
• Isoelectric Point: 8.8944

>NTDB_id=945130 ACFW98_RS01735 WP_015417447.1 367753..370164(+) (priA) [Bacillus amyloliquefaciens strain JP5008]
MNVAEVIVDVSSKNIDRPFDYKIPDHLKGMIEVGMRVIVPFGPRKLQGFVTGLKDSSELNGGSMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIKQLFRNQKSLLYSDITDESILSSLQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQASKQLAVLTYFIGGGAKISAAELCKKTDSSSATLKTLLKKGL
LTESFEEVYRDPYQDRMFKKTEPLSLTEEQQAAFEPIKQAVANHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGQEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSRELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQIQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=945130 ACFW98_RS01735 WP_015417447.1 367753..370164(+) (priA) [Bacillus amyloliquefaciens strain JP5008]
ATGAATGTAGCAGAAGTCATTGTCGATGTCAGCTCCAAAAACATTGACAGACCCTTTGATTACAAAATTCCCGATCATTT
AAAAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTTGGCCCGCGCAAGCTTCAAGGATTTGTGACCGGTTTGA
AAGATTCGTCTGAATTAAACGGCGGCTCAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACGCCCGTTCTGACTGAGGAA
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATCACGGCGCTTCAGGCAATGCTGCCCGCCGC
ACTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATTAAACAGCTTTTCAGGA
ATCAAAAAAGTCTTCTGTACTCGGATATTACCGATGAAAGCATTCTTTCGTCACTTCAAAAATTCGTGCGCAAAGGAAGC
ATTGATGTCACATATAAAGTCGCCCAAAAAACGAATAAAAAAATGATCCGCATGATCGAAGCGGGGGCGGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAGCAGGCTTCTAAACAGCTCGCCGTGCTTACTTATTTTATCGGCGGGGGAG
CAAAGATTTCCGCGGCCGAGCTTTGCAAAAAGACCGATTCCAGTTCCGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGAAAGCTTTGAGGAGGTTTACCGCGATCCTTATCAGGACAGAATGTTCAAAAAAACAGAGCCGCTCTCTCTTAC
AGAAGAGCAGCAGGCCGCTTTTGAGCCGATTAAACAAGCTGTAGCCAATCATGTCCATCACGTCTTTTTGCTTCACGGCG
TCACGGGAAGCGGCAAAACGGAAATTTACCTGCAATCCATTGAAAAAGTGCTGGCAAAAGGACAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCGTTGACACCGCAAATGGTCAACCGTTTTAAAGGCCGCTTCGGCTCACAGGTTGCGGTCATGCACAG
CGGATTATCCACAGGTGAAAAATACGATGAATGGCGGAAGATTCAGCGCAAGGAAGTCCGGCTTGTCGTCGGAGCCAGAT
CGGCCATCTTTGCCCCGTTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCTTCCTATAAGCAGGAGGAA
ATGCCGCGGTATCATGCAAAGGACGTAGCCATCCGCCGGGCCGAGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAGTCATTTGCGCGGGCGAAAAAAGGCGTTTATGAGCTGCTTCCGTTAAAACATCGCGTAAATCGCCAGC
TCATGCCTGACGTTTCTCTCGTTGATATGAGAGAAGAACTGAGAAACGGCAACAGATCAATGTTTTCAAGAGAGCTGATG
GAACAGCTGGAAGAGAAGCTTGCAAAAAAAGAGCAGGCCGTCCTTTTTCTGAATAAACGGGGGTATTCCTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCGCAATGTCCGCATTGCGACATTTCAATGACTTATCACCGCTACGGACAAAGGCTGA
AATGCCATTACTGCGGCCATGAGGAACCTGTCCCGGGAACATGCCCGGAATGCGGCAGCGAACATATCCGCTTTTTCGGC
ACGGGCACGCAGCGTGTGGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACCAC
TTCAAGAAAAGGCGCCCATGAAAAACTGTTGTCAGCGTTCGGTGAAGGGAAGGCGGATATCCTGCTCGGTACGCAAATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCTGATTTC
AGGGCCGCGGAGAAGACATTCCAGCTGCTGACGCAGGTAAGCGGACGGGCCGGGCGGCATGAAAAGCCGGGAACGGTGGT
CATTCAAACGTATACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCACGACTATGAAACTTTTTTTCAGCAGGAAA
TGGCGCACAGAAGGGCCCAGTCATACCCGCCTTATTATTACGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCGGCAAAA
GCCGCGGTCACCGCAGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGGCCTGATACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATCGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGATTC
AGTTGAAAAAGATACTGGAGCACTATAAACGCGACATTGCGCAAAAGCATGTCATGATTTCAATCGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	84.348	100	0.846