Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   ACFW99_RS13700 Genome accession   NZ_CP170651
Coordinates   2804537..2806894 (-) Length   785 a.a.
NCBI ID   WP_060674766.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2799537..2811894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS13675 (ACFW99_13675) - 2800043..2800822 (-) 780 WP_015417952.1 enoyl-CoA hydratase -
  ACFW99_RS13680 (ACFW99_13680) - 2800836..2801420 (-) 585 WP_007408119.1 TetR/AcrR family transcriptional regulator -
  ACFW99_RS13685 (ACFW99_13685) - 2801509..2803197 (-) 1689 WP_156240446.1 AMP-binding protein -
  ACFW99_RS13690 (ACFW99_13690) - 2803303..2803977 (+) 675 WP_156240447.1 DUF2711 family protein -
  ACFW99_RS13695 (ACFW99_13695) - 2804116..2804520 (-) 405 WP_003152541.1 DUF350 domain-containing protein -
  ACFW99_RS13700 (ACFW99_13700) mutS/mutS2 2804537..2806894 (-) 2358 WP_060674766.1 endonuclease MutS2 Machinery gene
  ACFW99_RS13705 (ACFW99_13705) polX 2806915..2808627 (-) 1713 WP_007408115.1 DNA polymerase/3'-5' exonuclease PolX -
  ACFW99_RS13710 (ACFW99_13710) - 2808733..2809266 (-) 534 WP_003152534.1 CvpA family protein -
  ACFW99_RS13715 (ACFW99_13715) zapA 2809274..2809531 (-) 258 WP_039063375.1 cell division protein ZapA -
  ACFW99_RS13720 (ACFW99_13720) rnhC 2809665..2810606 (+) 942 WP_060674770.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87260.62 Da        Isoelectric Point: 6.4547

>NTDB_id=944778 ACFW99_RS13700 WP_060674766.1 2804537..2806894(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain JP5025]
MQQKVLSSLEFHKVKEQITAHAASSLGREKLLQLKPLTDLSDIQKQLDEVEEASAVMRLRGHAPFGGLTDIRSALRRAEI
GSVLTPAEFTELSGLLYAVKQMKHFISQMTEDGVGIPLIQAHAEELITLGDLEREINSCIDDHGEVLDHASPALRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRMLTEHTAEHTQEIAQNVEVLQTLDFIFAKARYAKAMKATKPLMNGDGFIRLKKARHPLLPQDQVVAN
DIELGGDYSTIVITGPNTGGKTVTLKTLGLLTIMAQAGLHIPADEGSEAAVFDNVFADIGDEQSIEQSLSTFSSHMVNIV
NILKDVSENSLVLFDELGAGTDPQEGAALAMSILDEVHRTNARVLATTHYPELKAYGYNRQGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISRRLGLPEHIIGQAKSEMTAEHNEVDLMIASLEKSKKRADEELSETESIRKEAEKLHKELQQQIIE
LNAQKDKMMEEAEQKAAEKLEAAAKEAEQIIRELRSIKQEHRSFKEHELIDAKKRLGDAMPAFEKSKQPERKTEKKRELK
PGDEVKVLTFGQKGALLEKTGEKEWNVQIGILKMKVKEKDLEFLKSAPEPKKEKAITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGHPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTIVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=944778 ACFW99_RS13700 WP_060674766.1 2804537..2806894(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain JP5025]
GTGCAGCAAAAAGTGTTATCATCACTTGAATTTCATAAAGTAAAAGAACAGATTACCGCACATGCCGCCTCATCGCTCGG
CCGGGAGAAGCTTCTGCAGCTGAAACCTTTGACGGATCTCTCAGACATCCAAAAACAGCTGGATGAAGTCGAAGAAGCAT
CCGCGGTCATGCGCTTGAGAGGCCATGCTCCGTTCGGCGGCCTGACCGACATAAGATCCGCTTTACGGCGGGCGGAAATC
GGCAGTGTGCTGACGCCGGCGGAATTTACCGAATTGTCCGGGCTGCTGTATGCCGTTAAACAAATGAAGCATTTTATCAG
CCAAATGACAGAAGACGGCGTCGGCATTCCGCTGATACAGGCCCATGCAGAAGAACTGATTACATTGGGCGATTTGGAAA
GGGAGATTAACTCCTGCATTGATGACCATGGTGAAGTGCTTGATCATGCGTCTCCCGCTTTAAGGGGAATCCGCACGCAG
CTTAGGACACTTGAATCAAGAGTCAGAGACCGGCTTGAATCGATGCTGCGTTCATCTTCCGCTTCTAAAATGCTGTCTGA
CACCATCGTTACGATTCGGAATGACCGCTTTGTCATCCCGGTCAAACAGGAATACAGATCAAGCTACGGCGGAATTGTCC
ACGATACATCTTCATCCGGTGCGACACTGTTTATTGAACCGCAGGCGATCGTAGATATGAACAACTCGCTTCAGCAGGCG
AAAGTAAAAGAAAAACAGGAAATCGAACGGATTCTCCGGATGCTGACGGAGCATACGGCTGAACACACACAAGAAATCGC
TCAAAATGTGGAAGTGCTGCAGACACTGGATTTCATTTTCGCCAAAGCGAGATACGCAAAAGCGATGAAGGCGACAAAAC
CACTCATGAACGGAGACGGTTTTATCCGGCTGAAAAAAGCGCGCCATCCGCTGCTGCCCCAAGATCAAGTCGTGGCCAAC
GATATTGAACTGGGCGGAGATTATTCAACGATCGTCATCACGGGACCGAATACGGGCGGTAAAACCGTTACGTTAAAAAC
ACTCGGGCTGCTGACGATAATGGCGCAGGCAGGATTGCACATACCGGCTGATGAGGGATCGGAAGCAGCAGTATTTGACA
ATGTGTTTGCCGATATCGGCGATGAACAGTCGATCGAACAAAGTCTGAGTACGTTCTCATCCCACATGGTCAATATCGTC
AATATTTTAAAAGACGTGTCTGAAAACAGTCTTGTGCTGTTTGATGAACTCGGTGCCGGAACAGATCCGCAGGAAGGAGC
GGCGCTTGCCATGAGCATCCTTGACGAAGTTCACCGGACAAACGCCAGAGTGCTTGCGACAACCCATTATCCGGAACTGA
AAGCATACGGATATAACAGACAAGGCGTCATGAATGCCAGCGTTGAATTTGACATTGAAACGCTTTCGCCTACCTATAAG
CTTCTGATCGGAGTGCCGGGCCGAAGCAACGCATTTGAAATCTCAAGACGCCTTGGGCTTCCGGAGCATATCATCGGCCA
GGCGAAGTCAGAAATGACCGCCGAACATAACGAAGTCGATCTGATGATCGCATCCCTTGAAAAAAGCAAAAAAAGAGCGG
ATGAAGAGCTTTCTGAAACCGAATCAATCAGAAAAGAAGCAGAAAAACTGCACAAAGAGCTTCAGCAGCAAATCATTGAG
CTGAACGCCCAAAAAGATAAAATGATGGAAGAAGCCGAGCAGAAAGCTGCGGAAAAACTGGAAGCCGCCGCAAAAGAAGC
CGAACAGATCATCCGCGAACTCCGGTCCATCAAGCAAGAACACAGATCCTTTAAGGAACACGAGCTGATTGACGCCAAAA
AACGTCTCGGAGACGCGATGCCGGCTTTTGAAAAATCAAAGCAGCCGGAAAGAAAAACAGAGAAAAAACGCGAGCTCAAG
CCGGGCGACGAAGTAAAGGTGCTCACATTCGGACAAAAAGGGGCGCTGCTTGAGAAAACCGGCGAGAAAGAATGGAACGT
CCAAATCGGCATCCTTAAAATGAAGGTGAAGGAAAAAGATCTTGAGTTTCTCAAATCGGCTCCTGAACCGAAAAAAGAAA
AAGCAATCACCGCCGTAAAAGGGAAGGATTATCACGTATCCCTTGAACTTGACCTCAGGGGAGAGCGTTACGAAAACGCC
CTCAGCCGCGTCGAAAAATATTTAGATGACGCGGTGCTTGCCGGCCATCCGAGAGTGTCCATCATTCACGGAAAAGGCAC
GGGCGCCCTCCGAAAAGGGGTGCAGGATCTTCTGAAAAACCACCGCAGCGTGAAAAGCTCGCGGTTCGGGGAAGCGGGAG
AAGGAGGATCAGGCGTAACGATTGTCGAACTAAAATAA

Domains

Predicted by InterProScan.

Smr domain (Smr)

(710-785)

MutS domain V (MutS_V)

(331-512)

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

87.643

100

0.876