Detailed information NTG0944641

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACFMSI_RS09750
• Genome accession: NZ_CP170579
• Coordinates: 2000269..2002677 (-)
• Length: 802 a.a.
• NCBI ID: WP_000560853.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain JB2E56
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1995269..2007677

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFMSI_RS09725 (ACFMSI_09725)	rsmB	1995435..1996742 (-)	1308	WP_000572239.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
ACFMSI_RS09730 (ACFMSI_09730)	fmt	1996739..1997674 (-)	936	WP_000161301.1	methionyl-tRNA formyltransferase	-
ACFMSI_RS09735 (ACFMSI_09735)	-	1997667..1998155 (-)	489	WP_000985287.1	peptide deformylase	-
ACFMSI_RS09740 (ACFMSI_09740)	-	1998454..1998657 (+)	204	WP_001198022.1	TM2 domain-containing protein	-
ACFMSI_RS09745 (ACFMSI_09745)	-	1998795..1999766 (-)	972	WP_000751710.1	hypothetical protein	-
ACFMSI_RS09750 (ACFMSI_09750)	priA	2000269..2002677 (-)	2409	WP_000560853.1	primosomal protein N'	Machinery gene
ACFMSI_RS09755 (ACFMSI_09755)	coaBC	2002677..2003876 (-)	1200	WP_000722168.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ACFMSI_RS09760 (ACFMSI_09760)	rpoZ	2004092..2004310 (-)	219	WP_000933956.1	DNA-directed RNA polymerase subunit omega	-
ACFMSI_RS09765 (ACFMSI_09765)	gmk	2004310..2004933 (-)	624	WP_000368225.1	guanylate kinase	-
ACFMSI_RS09770 (ACFMSI_09770)	-	2005208..2006905 (+)	1698	WP_000312759.1	NFACT family protein	-
ACFMSI_RS09775 (ACFMSI_09775)	-	2007168..2007569 (-)	402	WP_000354182.1	VOC family protein	-

Sequence

Protein

• Length: 802 a.a.
• Molecular weight: 92535.44 Da
• Isoelectric Point: 6.7334

>NTDB_id=944641 ACFMSI_RS09750 WP_000560853.1 2000269..2002677(-) (priA) [Staphylococcus aureus strain JB2E56]
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKED
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKDRVFEQESKQQLTEDQFKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSNGERYDEWQKIRNGRARVSVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Nucleotide

• Length: 2409 bp

>NTDB_id=944641 ACFMSI_RS09750 WP_000560853.1 2000269..2002677(-) (priA) [Staphylococcus aureus strain JB2E56]
ATGATAGCGAAAGTCATAGTCGATGTCGCGTCGAAGAGCGTTGACTATAAATTTGATTATATAATTCCCGAACAACTCGA
ATCTGTCATCCAACCTGGTGTGCGTGTAATTGTACCTTTTGGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAG
CAGAACCTGATGCACAACTTGACGTTTCGAAGTTAAAAAAAATCATAGAAGTGAAAGATATACAACCAGAATTAACATCA
GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATGTCATTAAACGTATTTCTATGTTAGAAGTGATGCTTCCGAG
TGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATGAAAGATGACATAGAGGTACCTTCAGCTTTATTACAAAAATTTG
ATAAGCATGGTTACTATTATTATAAAGATGCGCAAAAAAATAATGATATTCAATTACTTATGAAGTTGTTAAAAGAGGAT
ATCGTTGAAGAAAAAACGATTCTCACACAAAATATAACTAAAAAAACTAAGCGTGCTGTTCGTGTCATTGAAGGGTATCA
TCCTGATGAAGTATTAGCTAAATTGGAGAAAGTTATTAAACAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATA
AAACAATATTTTTAACTGATATTGAGGATATGGGCTTTTCAAAATCCAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
GAAAAATATGACGCGGTTGTTGAAAGAGACCCATTTAAAGATCGTGTTTTCGAACAAGAATCAAAACAGCAATTAACAGA
AGACCAATTTAAAGCATATGAAGCGATTAAAGCTAAAATTGTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGA
CGGGATCAGGTAAAACAGAAGTATATTTACAAACGATAGAAGATGTTTTAAGCCAAGGAAAACAGGCGATGATGTTAGTT
CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGCTTCAAACGTCGATTTGGTGATGACGTTGCTGTATTACATTCTGG
CTTATCTAATGGGGAACGTTATGATGAGTGGCAAAAAATAAGGAATGGTCGTGCGAGAGTAAGTGTTGGTGCAAGGTCAA
GTGTGTTCGCACCTTTCAAAAATTTAGGATTAATCATTATTGATGAAGAACATGAATCTACATATAAACAAGAAGATTAT
CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGCCCAGTCATTTTAGGAAGTGCAACACC
ATGTCTTGAAAGTTATGCACGAGCTGAAAAAGGTGTTTATCATTTGCTATCATTACCAAACAGAGTGAACCAACAAGCTT
TACCTGAAATTGATATCGTAGACATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
GCCATACAATTAAGATTAGATCGACAGGAACAAGTTGTTTTATTTTTAAATCGACGTGGTTATGCATCATTTATGTTATG
TCGGGATTGTGGATATGTACCGCAATGCCCAAACTGTGATATTTCATTAACGTATCATAAAACGACAGACTTATTAAAAT
GTCACTATTGTGGTTACCAAGAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACT
GGTACTCAGAAAGTTGAAGAACTATTGCAACAAGAATTTGAAGATGCGCGCATAATTAGGATGGATGTAGATACAACCTC
AAAGAAAGGTGCACATGAAAAGTTATTGACTGAATTCGAAAAAGGTAACGGTGACATTTTACTAGGTACTCAGATGATTG
CGAAAGGATTAGATTATCCAAATATTACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
GCGAGCGAACGTACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGTCATGAAAAGGCAGGTCAAGTCATCAT
TCAAACGTATAATCCAGATCATTATTCAATATTGGATGTTCAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGG
AATATCGTAAATTAGGAAAGTATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
ATGGAAGCATCGCAACATGTTCATAAAATTTTATTACAGCATTTAACAGAAAAAGCGCTTGTACTAGGTCCATCTCCGGC
AGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATTTTAGTGAAATATAAAAGTGAACCTGGATTATTACAAGCCA
TTCAATTTTTAGATGACTATTACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCACAGATG
ATGATGTAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (264-427)

• Primosomal protein N C-terminal domain (PriA_C) (703-798)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (4-106)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (516-541)

• Helicase conserved C-terminal domain (Helicase_C) (564-659)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	50.433	100	0.509