Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   V9W63_RS05495 Genome accession   NZ_CP146760
Coordinates   1061780..1065277 (+) Length   1165 a.a.
NCBI ID   WP_338683987.1    Uniprot ID   -
Organism   Bacillus velezensis strain JJ334     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1056780..1070277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9W63_RS05470 (V9W63_05470) - 1056940..1058097 (-) 1158 WP_167340590.1 AbrB family transcriptional regulator -
  V9W63_RS05475 (V9W63_05475) - 1058265..1059461 (-) 1197 WP_007409175.1 MFS transporter -
  V9W63_RS05480 (V9W63_05480) - 1059477..1060097 (-) 621 WP_007409174.1 TetR/AcrR family transcriptional regulator -
  V9W63_RS05485 (V9W63_05485) - 1060348..1060857 (-) 510 WP_025284528.1 ferritin-like domain-containing protein -
  V9W63_RS05490 (V9W63_05490) - 1060926..1061639 (-) 714 WP_020955605.1 YetF domain-containing protein -
  V9W63_RS05495 (V9W63_05495) addB 1061780..1065277 (+) 3498 WP_338683987.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  V9W63_RS05500 (V9W63_05500) addA 1065264..1068968 (+) 3705 WP_338684078.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  V9W63_RS05505 (V9W63_05505) sbcD 1069033..1070205 (+) 1173 WP_113766359.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1165 a.a.        Molecular weight: 133347.83 Da        Isoelectric Point: 5.3571

>NTDB_id=944290 V9W63_RS05495 WP_338683987.1 1061780..1065277(+) (addB) [Bacillus velezensis strain JJ334]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHEFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHQYAPVACIESQYDVRPAAAYTGGQEAFTVMQAQNRR
AELEGIAREIQSLVRDGGYRYKDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRYE
AVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETREWI
AAPLVRLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQRAEEAGNMIEAQQHGQAWDAVIQLLEEFTGMMGEDEIS
LALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELSS
AGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQV
AQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHRRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNAC
QFSHFASHGLQLKERQFFKLDAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPKLQKEILLSSNR
HFYVKEKLQKIVTRVSGILSEHARASGFVPVGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIVD
YKSSDRGLDLAEVYYGLALQMLTYLDLSITHSEDWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGLL
LGDREAISLMDTTLEEGRSNIVNAGLKKDGSLRSDSAAVSERDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTPC
TYCAFQSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3498 bp        

>NTDB_id=944290 V9W63_RS05495 WP_338683987.1 1061780..1065277(+) (addB) [Bacillus velezensis strain JJ334]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGG
GGCATGAACCGCCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAATTGATTGAAGAACATAAGCACGAGTT
TAAAGTGTATCAAAAAGCGAGTGACAAAACCGGGTTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGATTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGACACTATTTGCATTCAGAGGATTATTT
GACGCTGCTCGCTCAGCAGATTCCTTTGGCGGACGTTGTAAAAGGCGCCCGTGTTTACGTCGACGGATTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGGCGGAACAGGCACCGGATGAGCTCGATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAATTGAATGCCGATATTTCCTGCAAGGAACTGCATGGCACGAAGCGGCATCAGTATGCGCCGGTTGCCTGTATAGAGT
CTCAGTACGATGTGCGTCCCGCCGCGGCGTATACCGGCGGACAGGAAGCTTTTACCGTCATGCAGGCGCAAAACAGAAGA
GCTGAGCTTGAAGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGATGGAGGATACCGGTACAAAGATATGGCGATTCT
CATCCGTCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCCGATTACGGGCTTCCTTATTTTATTGACGGAA
AGGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGTTCAAGCCTGGATGTCGTGAAGGGGAACTGGAGATATGAA
GCAGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTTGATCA
GCTTGAAAATTACTGTATCGCCTACGGCATTAAAGGGGAGCGCTGGACGAACGGCGAGCGGTTTGTATACAGGCGCTTCG
TATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAACACATGCTGAATGAGACGAGAGAGTGGATA
GCTGCACCCCTTGTCCGGCTTCAGAACCGGATGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAGGCTCTTTATCTCTT
TTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGGAAAAGGCAGCGGGCCGAAGAGGCCGGAAACATGATCGAAGCGC
AGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAGGAGTTTACCGGCATGATGGGTGAAGATGAAATATCT
TTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACGGAATCGCTTCATTTTTCTTTAATTCCTCCCGCGCTTGACCAAGT
ATTTGTAGGGAATATGGATCTCTCAAGAATGTACGGCACGTCCTGCACATTCGTCATCGGTGCCAATGACGGCGTTCTTC
CGGCGCGCCCGGATGAAAACGGCGTGCTGTCTGATGATGACAGGGAGTGGCTGAAAGCAGTCGGAGTGGAGCTGTCTTCC
GCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCGAGTCCGTCTGACCGTCTTTATGTGTC
ATACCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACAGGCTTGGCGAGCTGTTTCCCGATC
ATCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAGGTG
GCTCAGACATATACGGCAAGTCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTATGGTGGAGCGCATACAA
TGTGCTGATGAAAGAGCCTGATCACCGGCGTGCGAAAAAGCTGTTTTCAAGCTTGTTTTTCCGCAATGAGGCGAAGCGTC
TAGAACGGCCTGTTTCGCGCCAGCTTTACGGCGAGCATATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCCTGC
CAGTTCTCTCATTTTGCATCTCACGGGCTTCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGACGCGCCGGATATCGGCCA
GCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAGATC
AGTGCCGGAATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAAGCTGCAAAAAGAGATTCTGCTCAGCTCAAACAGA
CACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTTTCAGGCATATTAAGCGAGCATGCGAGGGCGAGCGG
TTTCGTCCCGGTCGGTCTTGAACTCGGTTTCGGCGGAAGCGGGCCGCTGCCTCCGCTCACTTTCACGCTGAAAAACGGCT
GTACGATGGAACTCGTCGGACGGATCGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGCCTGCTGCTGAGAATCGTTGAT
TATAAATCAAGCGACAGGGGGCTTGATCTGGCTGAGGTGTATTACGGGCTCGCTCTGCAAATGCTCACATATCTTGACTT
ATCGATTACACATTCCGAAGATTGGCTCGGTATGAAAGCGACCCCGGCAGGCGTTTTGTATTTCCATATTCATGATCCGA
TGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTTAAGAAATTTAAAATGAAAGGCCTTCTC
CTCGGTGACCGGGAGGCGATCAGCCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATCGTCAATGCCGGTTTGAA
AAAGGACGGTTCTTTAAGATCCGACTCAGCCGCGGTCAGCGAACGGGATTTTCATCTGCTGACAGACCATGTGCGGCGGA
CATTTGAACAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCTTGT
ACGTACTGTGCGTTTCAATCGGTGTGCCAGTTTGATGAGTCGCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGAAAA
AGACGGCACCATACTGGATTGGCTGAAGAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(302-668)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.19

100

0.833