Detailed information NTG0943634

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACFIOT_RS08340
• Genome accession: NZ_CP170414
• Coordinates: 1658091..1660499 (-)
• Length: 802 a.a.
• NCBI ID: WP_054190230.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain t5047
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1653091..1665499

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFIOT_RS08315	rsmB	1653258..1654565 (-)	1308	WP_054190225.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
ACFIOT_RS08320	fmt	1654562..1655497 (-)	936	WP_070016672.1	methionyl-tRNA formyltransferase	-
ACFIOT_RS08325	-	1655490..1655978 (-)	489	WP_054190227.1	peptide deformylase	-
ACFIOT_RS08330	-	1656277..1656480 (+)	204	WP_054190228.1	TM2 domain-containing protein	-
ACFIOT_RS08335	-	1656618..1657589 (-)	972	WP_054190229.1	hypothetical protein	-
ACFIOT_RS08340	priA	1658091..1660499 (-)	2409	WP_054190230.1	primosomal protein N'	Machinery gene
ACFIOT_RS08345	coaBC	1660499..1661698 (-)	1200	WP_054190231.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ACFIOT_RS08350	rpoZ	1661914..1662132 (-)	219	WP_000933956.1	DNA-directed RNA polymerase subunit omega	-
ACFIOT_RS08355	gmk	1662132..1662755 (-)	624	WP_000368225.1	guanylate kinase	-
ACFIOT_RS08360	-	1663030..1664727 (+)	1698	WP_054190232.1	NFACT family protein	-
ACFIOT_RS08365	-	1664984..1665384 (-)	401	Protein_1617	VOC family protein	-

Sequence

Protein

• Length: 802 a.a.
• Molecular weight: 92545.46 Da
• Isoelectric Point: 6.7334

>NTDB_id=943634 ACFIOT_RS08340 WP_054190230.1 1658091..1660499(-) (priA) [Staphylococcus aureus strain t5047]
MIAKVIVDIASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIVEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKIKDDIEVPSILLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKNRVFEQESKQQLTEDQFKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Nucleotide

• Length: 2409 bp

>NTDB_id=943634 ACFIOT_RS08340 WP_054190230.1 1658091..1660499(-) (priA) [Staphylococcus aureus strain t5047]
ATGATAGCGAAAGTCATAGTCGATATCGCATCGAAGAGCGTTGACTATAAATTTGATTATATAATTCCCGAACAACTTGA
ATCTGTCATCCAACCTGGTGTGCGTGTGATTGTACCTTTTGGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAG
CAGAACCTGATGCACAGCTTGACGTTTCGAAGTTAAAAAAAATCGTAGAAGTGAAAGATATACAACCAGAATTAACATCA
GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATGTTATTAAACGTATTTCTATGCTAGAAGTGATGCTTCCGAG
TGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATAAAAGATGACATAGAGGTACCTTCAATTTTATTACAAAAATTTG
ATAAGCATGGTTACTATTATTATAAAGATGCGCAAAAAAATAATGATATTCAATTGCTTATGAAGTTGTTAAAAGATGAT
ATCGTTGAAGAAAAAACGATTCTCACACAAAATATAACTAAAAAAACCAAGCGTGCTGTTCGTGTCATTGAAGGGTATCA
TCCTGATGAAGTATTAGCTAAGTTGGAGAAAGTTATTAAACAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATA
AAACAATATTTTTAACTGATATTGAGGATATGGGCTTTTCAAAATCCAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
GAAAAATATGATGCGGTTGTTGAAAGAGACCCATTTAAAAATCGTGTTTTCGAACAAGAATCAAAACAGCAATTAACAGA
AGACCAATTTAAAGCATATGAAGCGATTAAAGCTAAAATTGTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGA
CGGGATCAGGTAAAACAGAAGTATATTTACAAACGATAGAAGATGTTTTAAGTCAAGGAAAACAGGCGATGATGTTAGTT
CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGTTTCAAACGTCGATTTGGTGATGACGTTGCTGTATTACATTCTGG
CTTATCTAATGGGGAACGTTATGATGAGTGGCAAAAAATAAGGGATGGTCGTGCGAGAGTAAGTGTTGGTGCAAGATCAA
GTGTGTTCGCACCTTTCAAAAATTTAGGGTTAATCATCATTGATGAAGAACATGAATCTACATATAAGCAAGAAGATTAT
CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGCCCAGTCATTTTAGGAAGTGCAACACC
ATGTCTTGAAAGTTATGCACGAGCTGAAAAAGGTGTTTATCATTTGCTATCTTTACCAAACAGAGTAAACCAACAAGCTT
TACCTGAAATTGATATAGTAGACATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
GCCATACAATTAAGATTAGATCGACAGGAACAAGTTGTTTTATTTTTAAATCGACGTGGTTATGCATCATTTATGTTATG
TCGAGATTGTGGATATGTACCGCAATGTCCAAACTGTGATATTTCATTAACGTATCATAAAACGACAGACTTATTAAAAT
GTCACTATTGTGGTTACCAAGAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACT
GGTACTCAGAAAGTTGAAGAACTATTGCAACAAGAATTTGAAGATGCGCGCATAATTAGGATGGATGTAGATACAACCTC
AAAGAAAGGTGCACATGAAAAGTTATTGACTGAATTCGAAAAAGGTAACGGTGACATTTTACTAGGTACTCAGATGATTG
CGAAAGGATTAGATTATCCAAATATTACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
GCGAGCGAACGTACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGCCATGAAAAGGCAGGTCAAGTCATCAT
TCAAACGTATAATCCAGATCATTATTCAATATTGGATGTTCAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGG
AATATCGTAAATTAGGAAAGTATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
ATGGAAGCATCGCAGCATGTTCATAAAATTTTATTACAGCATTTAACAGAAAAGGCGCTTGTACTAGGTCCATCTCCGGC
AGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATTTTAGTGAAATATAAAAGTGAACCTGGATTATTACAAGCCA
TTCAGTTTTTAGATGACTATTACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCACAGATG
ATGATGTAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (264-427)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (4-106)

• Helicase conserved C-terminal domain (Helicase_C) (564-659)

• Primosomal protein N C-terminal domain (PriA_C) (703-798)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (516-541)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	50.309	100	0.507