Detailed information NTG0093758

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLID5_RS11025
• Genome accession: NZ_AP025701
• Coordinates: 2283685..2284110 (-)
• Length: 141 a.a.
• NCBI ID: WP_161935022.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain ID-5
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2278685..2289110

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLID5_RS10990 (LLID5_21630)	-	2278799..2279176 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
LLID5_RS10995 (LLID5_21640)	-	2279380..2280246 (+)	867	WP_251918763.1	RluA family pseudouridine synthase	-
LLID5_RS11000 (LLID5_21650)	-	2280289..2281098 (-)	810	WP_014570791.1	metal ABC transporter permease	-
LLID5_RS11005 (LLID5_21660)	-	2281091..2281828 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
LLID5_RS11010 (LLID5_21670)	-	2282005..2282847 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
LLID5_RS11015 (LLID5_21680)	-	2282844..2283281 (-)	438	WP_251918765.1	zinc-dependent MarR family transcriptional regulator	-
LLID5_RS11020 (LLID5_21690)	comGG	2283362..2283646 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
LLID5_RS11025 (LLID5_21700)	comGF	2283685..2284110 (-)	426	WP_161935022.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLID5_RS11030 (LLID5_21710)	comGE	2284094..2284390 (-)	297	WP_033900709.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLID5_RS11035 (LLID5_21720)	comGD	2284362..2284793 (-)	432	WP_012898621.1	competence type IV pilus minor pilin ComGD	Machinery gene
LLID5_RS11040 (LLID5_21730)	comGC	2284753..2285136 (-)	384	WP_012898622.1	competence type IV pilus major pilin ComGC	Machinery gene
LLID5_RS11045 (LLID5_21740)	comGB	2285150..2286169 (-)	1020	WP_251918767.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLID5_RS11050 (LLID5_21750)	comGA	2286117..2287055 (-)	939	WP_033900707.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16323.98 Da
• Isoelectric Point: 8.9981

>NTDB_id=93758 LLID5_RS11025 WP_161935022.1 2283685..2284110(-) (comGF) [Lactococcus lactis strain ID-5]
MNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFLYVTKDKK
LRFGFMRDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 426 bp

>NTDB_id=93758 LLID5_RS11025 WP_161935022.1 2283685..2284110(-) (comGF) [Lactococcus lactis strain ID-5]
TTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAATTTCTGGTTCAGTCCTTGTCAT
CTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAAAAGATTGGCAAATTTTCTGTC
AGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTGTATGTAACTAAAGACAAAAAA
CTAAGGTTTGGTTTTATGAGAGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTATCAGCCAATGCTTTATGATAT
AAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATCAAGGAGGTGAGAGAACATTTA
TCTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	98.582	0.752
comYF	Streptococcus mutans UA140	47.826	97.872	0.468
comYF	Streptococcus mutans UA159	47.101	97.872	0.461
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	97.163	0.404
comGF/cglF	Streptococcus mitis SK321	41.606	97.163	0.404
comGF/cglF	Streptococcus pneumoniae Rx1	39.007	100	0.39
comGF/cglF	Streptococcus pneumoniae D39	39.007	100	0.39
comGF/cglF	Streptococcus pneumoniae R6	39.007	100	0.39
comGF/cglF	Streptococcus pneumoniae TIGR4	39.007	100	0.39