Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   V5F86_RS13665 Genome accession   NZ_CP145137
Coordinates   2681372..2683732 (-) Length   786 a.a.
NCBI ID   WP_338442915.1    Uniprot ID   -
Organism   Bacillus spizizenii strain kcgeb_sc     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2676372..2688732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5F86_RS13645 (V5F86_13650) - 2676448..2678001 (+) 1554 WP_114151385.1 SH3 domain-containing protein -
  V5F86_RS13650 (V5F86_13655) dtd 2678028..2678468 (-) 441 WP_268454869.1 D-aminoacyl-tRNA deacylase -
  V5F86_RS13655 (V5F86_13660) relA 2678481..2680685 (-) 2205 WP_014114617.1 GTP diphosphokinase -
  V5F86_RS13660 (V5F86_13665) - 2680854..2681366 (-) 513 WP_014114618.1 adenine phosphoribosyltransferase -
  V5F86_RS13665 (V5F86_13670) recJ 2681372..2683732 (-) 2361 WP_338442915.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  V5F86_RS13670 (V5F86_13675) - 2683799..2684131 (-) 333 WP_079288037.1 lipopolysaccharide assembly LapA domain-containing protein -
  V5F86_RS13675 (V5F86_13680) - 2684209..2684706 (-) 498 WP_338442918.1 cation:proton antiporter regulatory subunit -
  V5F86_RS13680 (V5F86_13685) secDF 2684853..2687066 (-) 2214 WP_061186677.1 protein translocase subunit SecDF -
  V5F86_RS13685 (V5F86_13690) comN 2687105..2687401 (-) 297 WP_003222684.1 post-transcriptional regulator ComN -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88167.69 Da        Isoelectric Point: 4.9379

>NTDB_id=937431 V5F86_RS13665 WP_338442915.1 2681372..2683732(-) (recJ) [Bacillus spizizenii strain kcgeb_sc]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAADRIKQAISQQ
EQIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPDVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELVKLSGGDIGEATEETVGFQIAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLNAEDVPDLRNRLNEIADNTLTEEDFIPVQEVDLVCSVEDITVESITEMNLLSPFGMLN
PKPHVLVENAVLEDVRKIGANKNHVKMTVRNESSQLDCVGFNKGELEEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRMWEDTVSVLPSAKRAIVSFKEDSTTLLQTEDLRREVHVISSEDQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKTPERIYFIFLNQEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELARHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGVKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=937431 V5F86_RS13665 WP_338442915.1 2681372..2683732(-) (recJ) [Bacillus spizizenii strain kcgeb_sc]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTCGAAATGAAGGGCATGAAAGAAGCGGCAGATCGAATAAAACAGGCGATTTCACAACAA
GAACAGATTATGATATATGGCGATTATGACGCCGATGGAGTTACCAGTACGTCAGTGATGCTTCACACGCTGCAAAAGCT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGCTTTAAAGAAGGATATGGCCCTAATGAACAGGCGTTTCGTTCGATTA
AAGAACGGGGCTTCTCTCTGATTATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAAGGTGGCAAAGGAACTC
GGGCTGGATGTCATTATTACCGATCACCATGAGCCGGGACCGGAGCTTCCGGATGTCCATGCGATCGTCCACCCGAAGCA
GCCTGGCTGCACCTACCCGTTTAAAGAACTTGCCGGGGTCGGAGTTGCGTTTAAGCTTGCCCACGCTTTATTGGGCGAAC
TCCCGGATGAACTGCTTGACTTGGCGGCAATCGGCACCATTGCCGATCTTGTCCCGTTGCATGACGAAAACAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGTCGGACGAATCGGCTGGGCTTAAAAGAGCTGGTCAAGCTGTCAGGCGGAGACAT
CGGAGAAGCGACCGAAGAAACAGTCGGGTTCCAGATAGCACCAAGGCTGAATGCCGTCGGACGAATCGAACAGGCGGATC
CTGCTGTTCATTTGCTCATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCTGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGCAAAATGACGGATGAAGCGATTGAAATGGTTGAACAGCAAGGTCTCGATCAAACTGCAATCGT
CGTGGCCAAAGCCGGGTGGAATCCAGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGTTTCTATCGTCCGGCCA
TTGTGCTTGGCATTGATGAAGAAAAAGGGATCGCTAAAGGTTCGGCCCGAAGCATCAGAGGCTTTAATTTATTCGAGAGT
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCTGCGGGGATGACGTTGAATGCCGAAGATGT
GCCGGATTTGCGAAACAGGCTGAATGAAATAGCAGACAATACGCTCACGGAAGAGGATTTTATTCCTGTACAGGAAGTCG
ATCTCGTCTGCAGTGTCGAGGACATTACGGTTGAAAGCATCACAGAAATGAACTTGCTGTCCCCATTTGGCATGCTGAAC
CCGAAACCGCACGTTCTCGTAGAAAACGCTGTGCTGGAAGATGTCCGCAAGATCGGCGCTAATAAAAATCATGTGAAAAT
GACGGTCAGAAACGAGTCGTCTCAGCTTGATTGCGTCGGTTTTAATAAAGGTGAGCTCGAAGAAGGCATCGTCCCGGGTT
CAAGAATTTCAATTGTCGGTGAGATGTCTATCAACGAATGGAACAATAGAAAAAAACCGCAGCTAATGATTAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGATGTGGGAAGACACAGTTTCCGTCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACACTTCTTCAGACTGAAGATCTTCGCCGTGAAGTGCACGTCATCA
GCTCTGAAGATCAAGCGAAGGCGTTTGACCTTGATGGTGCATATATTGTATTGCTAGATCCGCCGCCTTCGTTGGACATG
CTCGCACGTTTGCTGGAAGGCAAGACGCCTGAGCGGATATATTTCATTTTTCTCAATCAAGAGGACCACTTTTTATCAAC
ATTTCCCGCAAGAGACCATTTTAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTAAAAAAACACGGAT
CAGAGCTTGCAAGACATAAAGGCTGGTCTGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTAGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTGGTTAGCGGAGTAAAGAAACGCGACCTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

97.455

100

0.975