Detailed information NTG0934334

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACCK16_RS05740
• Genome accession: NZ_CP168089
• Coordinates: 1182981..1185389 (+)
• Length: 802 a.a.
• NCBI ID: WP_000560834.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain CUBIST-23
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1177981..1190389

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACCK16_RS05715 (ACCK16_05715)	-	1178036..1178437 (+)	402	WP_000354184.1	VOC family protein	-
ACCK16_RS05720 (ACCK16_05720)	-	1178753..1180450 (-)	1698	WP_000312773.1	NFACT family protein	-
ACCK16_RS05725 (ACCK16_05725)	gmk	1180725..1181348 (+)	624	WP_000368225.1	guanylate kinase	-
ACCK16_RS05730 (ACCK16_05730)	rpoZ	1181348..1181566 (+)	219	WP_000933956.1	DNA-directed RNA polymerase subunit omega	-
ACCK16_RS05735 (ACCK16_05735)	coaBC	1181782..1182981 (+)	1200	WP_000722167.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ACCK16_RS05740 (ACCK16_05740)	priA	1182981..1185389 (+)	2409	WP_000560834.1	primosomal protein N'	Machinery gene
ACCK16_RS05745 (ACCK16_05745)	-	1185894..1186865 (+)	972	WP_000751710.1	hypothetical protein	-
ACCK16_RS05750 (ACCK16_05750)	-	1187003..1187206 (-)	204	WP_001198024.1	TM2 domain-containing protein	-
ACCK16_RS05755 (ACCK16_05755)	-	1187505..1187993 (+)	489	WP_000985294.1	peptide deformylase	-
ACCK16_RS05760 (ACCK16_05760)	fmt	1187986..1188921 (+)	936	WP_000161292.1	methionyl-tRNA formyltransferase	-
ACCK16_RS05765 (ACCK16_05765)	rsmB	1188918..1190225 (+)	1308	WP_000572243.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-

Sequence

Protein

• Length: 802 a.a.
• Molecular weight: 92550.46 Da
• Isoelectric Point: 6.6582

>NTDB_id=934334 ACCK16_RS05740 WP_000560834.1 1182981..1185389(+) (priA) [Staphylococcus aureus strain CUBIST-23]
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHIIKRISMLEVMLPSAIKAKYKKAFKMKDEIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKDRVFEQESKQQLTEDQFKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Nucleotide

• Length: 2409 bp

>NTDB_id=934334 ACCK16_RS05740 WP_000560834.1 1182981..1185389(+) (priA) [Staphylococcus aureus strain CUBIST-23]
ATGATAGCGAAAGTCATAGTCGATGTCGCGTCGAAGAGCGTTGACTATAAATTTGATTATATAATTCCTGAACAACTCGA
ATCTGTCATCCAACCTGGTGTGCGTGTAATTGTACCTTTTGGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAG
CAGAACCTGATGCACAACTTGACGTTTCGAAGTTAAAAAAAATCATAGAAGTGAAAGATATACAACCAGAATTAACATCA
GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATATCATTAAACGTATTTCTATGTTAGAAGTGATGCTTCCGAG
TGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATGAAAGATGAGATAGAGGTACCTTCAGCTTTATTACAAAAATTTG
ATAAGCATGGTTACTATTATTATAAAGATGCCCAAAAAAATAATGATATTCAATTGCTTATGAAGTTGTTAAAAGATGAT
ATCGTTGAAGAAAAAACGATTCTCACACAAAATATAACTAAAAAAACTAAGCGTGCTGTTCGTGTCATTGAAGGGTATCA
TCCTGATGAAGTATTAGCTAAATTGGAGAAAGTTATTAAACAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATA
AAACAATATTTTTAACTGATATTGAGGATATGGGCTTTTCAAAATCCAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
GAAAAATATGACGCGGTTGTTGAAAGAGACCCATTTAAAGATCGTGTTTTCGAACAAGAATCAAAACAGCAATTAACAGA
AGACCAATTTAAAGCATATGAAGCGATTAAAGCTAAAATTGTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGA
CGGGTTCAGGTAAAACAGAAGTATATTTACAAACGATAGAAGATGTTTTAAGCCAAGGAAAACAGGCCATGATGTTAGTT
CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGCTTCAAACGTCGATTTGGTGATGACGTTGCTGTATTACATTCTGG
CTTATCTAATGGGGAACGTTATGATGAGTGGCAAAAAATTAGGGATGGTCGTGCGAGAGTAAGTGTTGGTGCAAGGTCAA
GTGTGTTCGCACCTTTCAAAAATTTAGGTTTAATCATTATTGATGAAGAACATGAATCTACATATAAGCAAGAAGATTAT
CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGCCCAGTCATTTTAGGAAGTGCAACACC
ATGTCTTGAAAGTTATGCTCGAGCTGAAAAAGGTGTTTATCATTTGCTATCATTACCAAACAGAGTGAACCAACAAGCTT
TACCTGAAATTGATATAGTAGATATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
GCCATACAATTAAGATTAGATCGACAGGAACAAGTTGTTTTATTTTTAAATCGACGTGGTTATGCATCATTTATGTTATG
TCGGGATTGTGGTTATGTACCGCAATGCCCAAACTGTGATATTTCATTAACGTATCATAAAACGACAGACTTATTAAAAT
GTCACTATTGTGGTTACCAAGAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACA
GGAACACAAAAAGTTGAAGAACTATTGCAGCAAGAATTTGAAGATGCGCGCATAATTAGGATGGATGTAGATACAACCTC
AAAGAAAGGTGCACATGAAAAGTTATTGACTGAATTCGAAAAAGGTAACGGTGACATTTTACTAGGTACTCAGATGATTG
CGAAAGGATTAGATTATCCAAATATTACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
GCGAGTGAACGTACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGTCATGAAAAGGCAGGTCAAGTCATCAT
TCAAACGTATAACCCAGATCATTATTCAATATTGGATGTTCAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGG
AATATCGTAAATTAGGAAAGTATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
ATGGAAGCATCACAGCATGTTCATAAAATTTTATTACAGCATTTAACGGAAAAAGCGCTTGTACTAGGTCCATCTCCGGC
AGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATTTTAGTGAAATATAAAAGTGAACCTGGATTATTACAAGCCA
TTCAATTTTTAGATGACTATTACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCACAGATG
ATGATGTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (564-659)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (4-106)

• Primosomal protein N C-terminal domain (PriA_C) (703-798)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (516-541)

• Type III restriction enzyme, res subunit (ResIII) (264-427)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	50.433	100	0.509