Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MWM02_RS04810 Genome accession   NZ_AP025627
Coordinates   947064..949421 (+) Length   785 a.a.
NCBI ID   WP_064549718.1    Uniprot ID   A0A1B7KWA9
Organism   Parageobacillus sp. KH3-4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 942064..954421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM02_RS04790 (PspKH34_09020) - 942078..942452 (-) 375 WP_064549714.1 TIGR04086 family membrane protein -
  MWM02_RS04795 (PspKH34_09030) spoVB 942521..944083 (-) 1563 WP_244403058.1 stage V sporulation protein B -
  MWM02_RS04800 (PspKH34_09040) - 944201..944497 (+) 297 WP_244403059.1 post-transcriptional regulator -
  MWM02_RS04805 (PspKH34_09050) secDF 944681..946933 (+) 2253 WP_064549717.1 protein translocase subunit SecDF -
  MWM02_RS04810 (PspKH34_09060) recJ 947064..949421 (+) 2358 WP_064549718.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MWM02_RS04815 (PspKH34_09070) - 949421..949933 (+) 513 WP_003248934.1 adenine phosphoribosyltransferase -
  MWM02_RS04820 (PspKH34_09080) - 950130..952328 (+) 2199 WP_064549719.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MWM02_RS04825 (PspKH34_09090) dtd 952343..952801 (+) 459 WP_064549720.1 D-aminoacyl-tRNA deacylase -
  MWM02_RS04830 (PspKH34_09100) - 952912..953097 (+) 186 WP_064549721.1 hypothetical protein -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 88099.34 Da        Isoelectric Point: 4.9127

>NTDB_id=93284 MWM02_RS04810 WP_064549718.1 947064..949421(+) (recJ) [Parageobacillus sp. KH3-4]
MLIAKTRWDVKRPDESIVKHLVEQLRIDPLIATLLVHRGVRTAEEANAFLYPLKQPFHDPFLLDGMERAIERIQQAIQDG
ERILVYGDYDADGVSSTTVMVSALKEAGAIVDFYIPNRFTEGYGPNEKAFRWAKEQGFSLIITVDNGIAAVKEIALANEL
GMDVIITDHHETGPALPEAYEIIHPKKPGSTYPFRELAGVGVAFKVAHALLGDVPRHLLDVVALGTIADLVPLTGENRIL
VAQGLEQLRNVKRIGLRTLCKQCGIDAEKIDEQTVGFVIAPRMNAVGRLGEADPAVRLLMTEDEEEAKRLAKEMDDLNRE
RQQLVAEIAEEAAQMIREQFPPSENKVLVVAKEGWNPGVIGIVASRLVEQFYRPTIVLSVDAEKGIAKGSARSIHGFDIF
SSLSACRDILPHFGGHPMAAGMTLSLEDVDELRQRLNALADDMLTEDDFTPITSIDAVCSVSDLTMEVAEQLEKFAPFGV
GNAKPLFLMENVSVETIRRVGADQSHLKAVFAQNGAAIDSVGFGFGYLRDEIAPDAKVSVVGELVINEWNHLRKPQLMIY
DMAVHECQLFDIRGMRDVRPLIAALPPDKRMLIMFREDTADALGIETYKEEICYAASVEEASQLCLDGRYVVLLDMPPSL
EIIKMLLRSSLPARIYALFYQRETHFFRTLPTREHFKWFYAFLRKQGMFNLAKYGGELARARGWTEETIWFMAKVFLELE
FITQRDGIVSLVPQPAKRDLSESPTYRLKQAQYELESLFLYSTYEQLKRCLFEMKGSQTYEEANA

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=93284 MWM02_RS04810 WP_064549718.1 947064..949421(+) (recJ) [Parageobacillus sp. KH3-4]
ATGTTAATCGCGAAAACACGTTGGGATGTGAAGCGCCCCGATGAAAGTATTGTAAAGCATTTAGTGGAACAATTGCGGAT
CGATCCGCTTATTGCAACGCTTCTTGTCCATCGCGGAGTTCGTACGGCAGAAGAAGCAAATGCGTTTTTATATCCGCTGA
AACAGCCGTTTCATGATCCGTTTTTGCTAGATGGAATGGAGCGGGCGATCGAGCGGATTCAACAGGCGATACAGGATGGG
GAGCGTATTTTAGTATATGGTGATTATGATGCAGACGGTGTAAGCAGCACGACGGTAATGGTCAGCGCTTTGAAGGAAGC
TGGCGCTATTGTTGATTTTTATATCCCCAACCGCTTTACCGAAGGATACGGTCCGAATGAAAAAGCGTTTCGTTGGGCAA
AGGAGCAAGGGTTTTCTTTGATTATTACGGTCGATAACGGCATTGCCGCGGTGAAGGAAATAGCGCTTGCCAATGAGCTT
GGAATGGATGTCATTATTACAGACCATCATGAAACGGGGCCGGCGTTGCCGGAAGCGTATGAAATTATTCATCCGAAAAA
GCCTGGAAGTACATATCCGTTTCGGGAATTAGCCGGGGTAGGAGTGGCGTTTAAAGTTGCGCATGCACTGCTCGGGGATG
TGCCGCGCCATTTATTGGATGTCGTCGCACTTGGAACAATCGCCGATCTCGTTCCCCTTACTGGCGAAAATCGAATATTG
GTGGCGCAAGGGCTTGAGCAGTTGCGAAATGTAAAACGTATTGGATTGCGTACGTTGTGTAAACAATGTGGAATAGACGC
AGAAAAAATCGACGAGCAGACGGTGGGGTTTGTCATTGCGCCGCGGATGAACGCGGTCGGGCGATTGGGGGAAGCCGATC
CTGCCGTAAGATTGCTAATGACGGAGGACGAAGAGGAAGCAAAAAGGCTTGCGAAAGAAATGGATGATTTAAATAGGGAG
CGGCAGCAGCTTGTCGCGGAAATAGCGGAAGAAGCGGCGCAAATGATACGGGAACAATTTCCGCCATCCGAGAATAAAGT
GCTTGTGGTGGCAAAAGAAGGATGGAACCCTGGAGTCATCGGCATTGTCGCATCCCGGCTGGTCGAACAGTTTTACCGCC
CGACGATCGTGTTAAGCGTTGATGCAGAAAAAGGCATCGCGAAAGGCTCGGCGCGAAGCATTCACGGGTTTGACATATTT
TCTAGCTTATCGGCATGCCGCGATATTTTGCCGCATTTTGGCGGCCATCCGATGGCGGCGGGAATGACGCTGTCTCTTGA
GGACGTTGACGAATTGCGCCAGCGTCTTAACGCGCTCGCCGACGATATGTTGACAGAAGATGATTTTACGCCAATTACGT
CGATTGACGCGGTTTGTTCCGTATCTGATTTAACGATGGAGGTAGCCGAACAGCTTGAAAAATTTGCTCCGTTTGGCGTC
GGTAATGCAAAGCCTCTTTTTTTAATGGAAAATGTGTCAGTGGAAACGATACGCCGCGTCGGCGCTGATCAATCGCATTT
AAAGGCCGTTTTTGCGCAAAATGGCGCTGCGATTGATAGCGTTGGTTTCGGCTTCGGGTATTTGCGCGATGAAATCGCTC
CAGATGCGAAAGTATCGGTTGTTGGCGAGTTAGTTATTAATGAGTGGAATCATCTTCGCAAGCCGCAGTTGATGATTTAT
GATATGGCGGTGCATGAATGCCAGCTGTTTGATATTCGCGGTATGCGCGATGTAAGACCGCTGATTGCGGCGCTTCCGCC
AGATAAGCGGATGCTCATTATGTTTCGCGAAGATACGGCGGATGCGTTAGGGATCGAAACATATAAAGAAGAAATTTGTT
ACGCCGCTTCAGTGGAAGAAGCATCGCAGCTTTGTTTAGATGGCCGCTACGTTGTCCTGCTGGATATGCCTCCGTCTTTG
GAAATCATAAAAATGTTGTTACGTTCTAGTTTACCTGCGCGCATTTATGCGTTATTCTATCAAAGGGAGACTCATTTTTT
CCGCACGTTGCCGACGCGTGAACATTTTAAATGGTTTTACGCGTTTTTGCGGAAACAGGGCATGTTTAATTTGGCGAAGT
ACGGCGGAGAGTTGGCGCGTGCGCGTGGATGGACGGAAGAAACCATCTGGTTTATGGCGAAAGTGTTTCTTGAACTGGAA
TTTATTACGCAACGGGATGGCATCGTGTCGCTTGTTCCTCAGCCAGCTAAACGAGATTTAAGCGAATCACCGACTTATCG
CTTGAAACAAGCTCAGTATGAGCTGGAAAGTCTTTTTTTGTATTCAACATATGAACAATTAAAGCGTTGTCTTTTTGAAA
TGAAAGGTTCGCAAACATATGAGGAGGCAAATGCATAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(305-439)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-771)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B7KWA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

54.534

99.745

0.544

Multiple sequence alignment