Detailed information NTG0931918

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V2192_RS07705
• Genome accession: NZ_CP143906
• Coordinates: 1643567..1644715 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain PA15_L5_37.22_ST773_VIM2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1638567..1649715

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V2192_RS07680 (V2192_07680)	-	1639718..1640014 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
V2192_RS07685 (V2192_07685)	-	1640011..1640604 (-)	594	WP_003084544.1	YggT family protein	-
V2192_RS07690 (V2192_07690)	proC	1640615..1641436 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
V2192_RS07695 (V2192_07695)	-	1641448..1642140 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
V2192_RS07700 (V2192_07700)	pilT	1642355..1643389 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
V2192_RS07705 (V2192_07705)	pilU	1643567..1644715 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
V2192_RS07710 (V2192_07710)	czcD	1644723..1645622 (-)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
V2192_RS07715 (V2192_07715)	-	1645777..1646181 (+)	405	WP_003084560.1	NINE protein	-
V2192_RS07720 (V2192_07720)	-	1646424..1647797 (+)	1374	WP_023098360.1	pyridoxal-phosphate dependent enzyme	-
V2192_RS07725 (V2192_07725)	-	1647794..1648978 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=931918 V2192_RS07705 WP_003110016.1 1643567..1644715(+) (pilU) [Pseudomonas aeruginosa strain PA15_L5_37.22_ST773_VIM2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=931918 V2192_RS07705 WP_003110016.1 1643567..1644715(+) (pilU) [Pseudomonas aeruginosa strain PA15_L5_37.22_ST773_VIM2]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382