Detailed information NTG0931792

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V2199_RS14805
• Genome accession: NZ_CP143899
• Coordinates: 3174868..3176016 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA26_WM3_03.23_ST27
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3169868..3181016

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V2199_RS14785 (V2199_14785)	-	3170606..3171790 (-)	1185	WP_023114752.1	cystathionine gamma-synthase	-
V2199_RS14790 (V2199_14790)	-	3171787..3173160 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
V2199_RS14795 (V2199_14795)	-	3173403..3173807 (-)	405	WP_003084560.1	NINE protein	-
V2199_RS14800 (V2199_14800)	czcD	3173961..3174860 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
V2199_RS14805 (V2199_14805)	pilU	3174868..3176016 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
V2199_RS14810 (V2199_14810)	pilT	3176194..3177228 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
V2199_RS14815 (V2199_14815)	-	3177443..3178135 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
V2199_RS14820 (V2199_14820)	proC	3178147..3178968 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
V2199_RS14825 (V2199_14825)	-	3178979..3179572 (+)	594	WP_003084544.1	YggT family protein	-
V2199_RS14830 (V2199_14830)	-	3179569..3179865 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=931792 V2199_RS14805 WP_003100959.1 3174868..3176016(-) (pilU) [Pseudomonas aeruginosa strain PA26_WM3_03.23_ST27]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=931792 V2199_RS14805 WP_003100959.1 3174868..3176016(-) (pilU) [Pseudomonas aeruginosa strain PA26_WM3_03.23_ST27]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTTGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382