Detailed information NTG0931026

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACB332_RS06285
• Genome accession: NZ_CP167016
• Coordinates: 1257172..1259556 (-)
• Length: 794 a.a.
• NCBI ID: WP_044559857.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain Isolate 10
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1252172..1264556

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACB332_RS06265 (ACB332_06265)	pknB	1252190..1254088 (-)	1899	WP_063812253.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
ACB332_RS06270 (ACB332_06270)	-	1254085..1254825 (-)	741	WP_063812254.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
ACB332_RS06275 (ACB332_06275)	rsmB	1254863..1256185 (-)	1323	WP_032465161.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
ACB332_RS06280 (ACB332_06280)	fmt	1256175..1257110 (-)	936	WP_115243149.1	methionyl-tRNA formyltransferase	-
ACB332_RS06285 (ACB332_06285)	priA	1257172..1259556 (-)	2385	WP_044559857.1	primosomal protein N'	Machinery gene
ACB332_RS06290 (ACB332_06290)	rpoZ	1259621..1259938 (-)	318	WP_002983650.1	DNA-directed RNA polymerase subunit omega	-
ACB332_RS06295 (ACB332_06295)	gmk	1259954..1260589 (-)	636	WP_002983649.1	guanylate kinase	-
ACB332_RS06300 (ACB332_06300)	-	1260699..1262306 (-)	1608	WP_011018058.1	ribonuclease Y	-
ACB332_RS06305 (ACB332_06305)	-	1262436..1263331 (-)	896	Protein_1186	LysR family transcriptional regulator	-

Sequence

Protein

• Length: 794 a.a.
• Molecular weight: 90445.43 Da
• Isoelectric Point: 9.4590

>NTDB_id=931026 ACB332_RS06285 WP_044559857.1 1257172..1259556(-) (priA) [Streptococcus pyogenes strain Isolate 10]
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKAIQAGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVTYFVTNHLIRIDERQI
DRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKTIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSDNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL

Nucleotide

• Length: 2385 bp

>NTDB_id=931026 ACB332_RS06285 WP_044559857.1 1257172..1259556(-) (priA) [Streptococcus pyogenes strain Isolate 10]
ATGGAAAAAGTAGCTCATGTGATTGTAGATATTCCATTGATGCAAACGGACAAGCCATTTTCTTATGGTATTCCAAAAGA
GTTAGTTTCTCTCGTGCAGCTTGGTTCTCGTGTGCATGTTCCTTTTGGAAAAGGGAATCGTTTGTTGCAAGGCTTTATTA
TTGGTTTTGGCCAAGAAGATTCAAGCTCTCTTAAACTGATTCAGACCGTTTTAGATCCTGAGCCAGTCCTAAACCAAGAA
CAATTGACCCTTGCTGATCAGCTTCGAAAAACAGTTTTTTCTTATAAAATCACGCTCTTAAAAGCAATGATTCCTAATCT
TCTTAACTCTAACTATGATAAAGTGCTAAGGCCAGAGTCAGGTCTAAAAAAGCCTGATAGAGACTTTCTTTTTGAAGGAA
AACCATCTGTCTTATATTCTACCTTAGACCGCGAGAAGGAAAAAATAGCTCTTAAGGCCATACAAGCTGGGCATATTACT
GTTAGTTATTTAGCCAAAGACAAGAAAAACCTAAAGACAGAAAAATATTATCACGTCGACTTAGACGCCTTAGCGGTACA
TCCCATCTCTAGTCGTGCCAAAAAGCGTCAATTATTAAAAGACTATCTGCTAACCCATACGAAGGAAGCTAAACTTGCAA
CGCTTTATCAAGCCTTTTCTAGAGATGTGGTGACTTACTTTGTGACAAATCACTTAATTCGTATTGACGAACGTCAAATT
GATCGCAGTGAGCCCTATTTTGACCAGATCAAACCATCTTCTTTTTTAACCTTAAATGAACAGCAAGCGTCAGCTGTTAC
TGAAATTGTTGAGCAAATTGGGAAACCGAGTAAACCTTTTTTGATTGAAGGGATTACAGGTTCGGGTAAGACAGAAGTTT
ATCTTCATATCATCGAAGCAGTTTTAAAACAGGATAAGACAGCCATTGTTTTAGTACCAGAAATTTCGTTGACTCCTCAG
ATGACCTCTCGTTTTATTTCCCGTTTCGGAAAGCAAGTGGCTATTATGCATTCAGGCTTATCAGATGGTGAAAAGTTTGA
TGAGTGGCGCAAGATAAAAACAGGACAGGCTAAGGTTGTGGTTGGTGCCCGCTCTGCTATTTTCTCCCCACTAGAGCGGA
TTGGTGCAATTATTATCGATGAGGAGCATGAATCAACCTATAAACAAGAAAGCAACCCTAGATACCATGCCAGAGAGGTT
GCCCTGCTCAGAGCTAAACACCATCAAGCAGTTGTGGTTATGGGCTCTGCTACGCCAAGTATTGAAAGCCGAGCAAGGGC
TAGTAAGGGGGTCTATCATTTTATTCAATTAACGCAAAGAGCAAACCCTCTTGCTAAGATTCCAGAAGTAACGATTGTTG
ATTTTCGTGATTATATTGGACAGCAAGCAGTTAGTAATTTTACCCCATATTTGATTGATAAGATTAAAGAACGTTTGGTT
AAAAAAGAACAAGTTGTCTTAATGTTAAATAGACGTGGCTATTCTAGTTTTGTGATGTGTCGGGACTGTGGTTATGTGGA
CAAATGTCCCAACTGTGATATCTCTTTAACCCTTCACATGGACACTAAAACTATGAATTGCCATTACTGTGGGTTTCAAA
AAACTATCCCAATTACTTGTCCTGAGTGTCATAGCAATAGTATCCGCTATTATGGCACTGGAACTCAAAAAGCTTTTGAT
GAATTGCAAGGGGTTATCCCAGAAGCTAGGATCTTGAGAATGGATGTTGACACTACACGAAAAAAAAGAAGTCACAAAAC
CATACTAGATAGTTTTGGTCGCCAGGAAGCAGATATTTTGTTAGGGACGCAAATGATTGCCAAAGGTCTGGATTTTCCAA
ATGTGACTTTAGTTGGGGTACTAAATGCAGATACCTCCTTAAATTTGCCAGATTTTCGAGCGTCAGAAAAAACGTTTCAA
TTGTTAACACAGGTTGCTGGTCGGGCAGGCCGTGCCCATAAGCCAGGAGAAGTCTTGATTCAAACTTATAATCCCGATCA
CTATGCTATTCAATTAGCTAAGAAACAAGATTTTGAAGCCTTTTACCGTTATGAAATGGCGATTCGCCGTCAGATGGCTT
ATCCCCCTTACTACTTTACAGTGGGTATTACGCTTTCGCATCGTTTAGAAGCATCGGTTGTAAAAAAAGCTTATCAAGTT
ACAGAACTGCTAAAGAGTCACTTATCGGATAATATTAAAATATTAGGACCGACTCCTAAGCCAATTGCTAGAACACACAA
TTTATACCACTATCAAATTTTGTTAAAATACCGTTTTGAAGATAACCTAGAAGAGACGCTCAATCGTATTCTTGATTGGA
GTCAAGAAGCAGATAATCGTCATTTAAAACTAATTATTGATTGTGAACCGCAACAATTTTTATAA

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (509-534)

• Primosomal protein N C-terminal domain (PriA_C) (696-790)

• Helicase conserved C-terminal domain (Helicase_C) (568-652)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-105)

• Type III restriction enzyme, res subunit (ResIII) (257-420)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.146	100	0.479