Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   SNM_RS03315 Genome accession   NZ_AP025613
Coordinates   578564..579697 (-) Length   377 a.a.
NCBI ID   WP_011173432.1    Uniprot ID   -
Organism   Thermus thermophilus strain SNM6-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 573564..584697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SNM_RS03295 (TthSNM66_06420) pilQ 574232..576505 (-) 2274 WP_244347033.1 secretin N-terminal domain-containing protein Machinery gene
  SNM_RS03300 (TthSNM66_06430) pilW 576502..577380 (-) 879 WP_244344967.1 competence protein Machinery gene
  SNM_RS03305 (TthSNM66_06440) pilO 577377..577958 (-) 582 WP_011173434.1 type 4a pilus biogenesis protein PilO Machinery gene
  SNM_RS03310 (TthSNM66_06450) pilN 577948..578571 (-) 624 WP_011173433.1 competence protein Machinery gene
  SNM_RS03315 (TthSNM66_06460) pilM 578564..579697 (-) 1134 WP_011173432.1 type IV pilus assembly protein PilM Machinery gene
  SNM_RS03320 (TthSNM66_06470) - 579832..580836 (-) 1005 WP_011173431.1 homoisocitrate dehydrogenase -
  SNM_RS03325 (TthSNM66_06480) - 580891..581382 (-) 492 WP_011173430.1 DUF5317 domain-containing protein -
  SNM_RS03330 (TthSNM66_06500) - 581433..582869 (-) 1437 WP_223965606.1 HD domain-containing phosphohydrolase -
  SNM_RS03345 (TthSNM66_06510) - 583644..584009 (+) 366 WP_244347034.1 YDG/SRA domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41399.02 Da        Isoelectric Point: 4.3805

>NTDB_id=93037 SNM_RS03315 WP_011173432.1 578564..579697(-) (pilM) [Thermus thermophilus strain SNM6-6]
MFKSLSQLFRPRVEALGLEIGASALKLVEVSGNPPALKALASRPTPPGLLMEGMVAEPAALAQEIKELLLEARTRKRYVV
TALSNLAVILRPIQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLTPLSEVQEGEQVQVMVAAARQEAVAGVLEAL
RGAGLVPVVLDVKPFAGLYPLEARLAEEPDRVFLVLDIGAESTSLVLLRGDKPLAVRVLTLSGKDFTEAIARSFNLDLLA
AEEVKRTYGMATLPTEDEELLLDFDAERERYSPGRIYDAIRPVLVELTQELRRSLEFFRIQLEEASPEMGYLLGGGSKLR
GLASLLTDTLGVNFEPVNPWEAVAVDPKRFESEQLQEIGPEFAVALGLALRGVEPLD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=93037 SNM_RS03315 WP_011173432.1 578564..579697(-) (pilM) [Thermus thermophilus strain SNM6-6]
GTGTTCAAAAGCCTTAGCCAGCTCTTCCGACCCAGGGTTGAGGCCCTGGGGTTGGAGATCGGGGCCTCCGCCCTGAAGCT
CGTGGAGGTGTCCGGGAACCCCCCTGCCCTCAAGGCCCTGGCCTCCCGCCCCACCCCGCCTGGCCTCCTGATGGAAGGGA
TGGTGGCCGAGCCCGCCGCGCTGGCCCAGGAGATCAAGGAGCTTCTCCTCGAGGCCCGCACCCGCAAGCGCTACGTGGTC
ACCGCCCTCTCCAACCTGGCGGTCATCCTCCGCCCCATCCAGGTCCCCAAGATGCCCCTCAAGGAGATGGAGGAGGCGGT
GCGCTGGGAGGCGGAGCGCTACATTCCCTTCCCCATTGACGAGGTGGTCCTGGACTTCGCCCCCCTGACCCCCCTCTCCG
AGGTGCAGGAGGGGGAGCAGGTCCAGGTAATGGTGGCGGCGGCGCGGCAGGAGGCGGTGGCGGGGGTCCTCGAAGCCCTG
AGGGGGGCGGGGCTCGTCCCCGTGGTGCTGGACGTGAAGCCCTTCGCCGGGCTTTACCCTCTGGAGGCCAGGCTTGCGGA
GGAGCCCGACCGGGTCTTCCTCGTCCTGGACATCGGGGCCGAGAGCACGAGCCTCGTCCTGCTCCGGGGGGACAAGCCCC
TGGCGGTGCGCGTCCTCACCCTCTCGGGCAAGGACTTCACCGAGGCCATCGCCCGAAGCTTCAACCTGGACCTCCTCGCC
GCCGAGGAGGTGAAGCGGACCTACGGGATGGCCACCCTCCCCACCGAGGACGAGGAGCTCCTCCTGGACTTTGACGCCGA
GCGGGAGCGCTACAGCCCGGGGCGCATCTATGACGCCATCCGCCCGGTGCTGGTGGAGCTCACCCAGGAGCTCCGCCGTA
GCCTTGAGTTCTTCCGCATCCAGCTGGAGGAGGCCTCGCCCGAGATGGGCTACCTCCTGGGCGGGGGGAGCAAGCTCAGG
GGGCTCGCCTCCCTCCTCACCGACACCTTGGGGGTGAACTTTGAGCCCGTCAACCCTTGGGAGGCGGTGGCGGTGGACCC
CAAGCGCTTTGAGTCGGAGCAACTCCAGGAGATCGGGCCGGAGTTCGCCGTGGCCCTGGGCTTGGCGTTGAGGGGGGTGG
AGCCCCTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

100

100

1

Multiple sequence alignment