Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   AB6W33_RS02185 Genome accession   NZ_CP163561
Coordinates   365522..367912 (+) Length   796 a.a.
NCBI ID   WP_000101528.1    Uniprot ID   Q8E1N9
Organism   Streptococcus agalactiae strain GBS-B4009     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 360522..372912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB6W33_RS02155 (AB6W33_02160) - 361147..361887 (+) 741 WP_001217938.1 ABC transporter permease -
  AB6W33_RS02160 (AB6W33_02165) - 361884..362969 (+) 1086 WP_000791349.1 sensor histidine kinase -
  AB6W33_RS02165 (AB6W33_02170) - 362971..363561 (+) 591 Protein_356 DNA-binding response regulator -
  AB6W33_RS02170 (AB6W33_02175) - 363611..364315 (+) 705 WP_001168922.1 TMEM175 family protein -
  AB6W33_RS02175 (AB6W33_02180) gmk 364482..365111 (+) 630 WP_050265446.1 guanylate kinase -
  AB6W33_RS02180 (AB6W33_02185) rpoZ 365134..365448 (+) 315 WP_000979239.1 DNA-directed RNA polymerase subunit omega -
  AB6W33_RS02185 (AB6W33_02190) priA 365522..367912 (+) 2391 WP_000101528.1 primosomal protein N' Machinery gene
  AB6W33_RS02190 (AB6W33_02195) fmt 367959..368894 (+) 936 WP_000163893.1 methionyl-tRNA formyltransferase -
  AB6W33_RS02195 (AB6W33_02200) rsmB 368884..370206 (+) 1323 WP_001262599.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  AB6W33_RS02200 (AB6W33_02205) - 370244..370981 (+) 738 WP_000406233.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -

Sequence

Protein


Download         Length: 796 a.a.        Molecular weight: 91170.95 Da        Isoelectric Point: 8.6523

>NTDB_id=926767 AB6W33_RS02185 WP_000101528.1 365522..367912(+) (priA) [Streptococcus agalactiae strain GBS-B4009]
MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGVRVHVPFGRGNRLLQGFVVGFRDDDELETKDIAEVLDFEPVLNQ
EQLDLADQMRHTVFSYKISILKSMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
EVQYLAKDKKTIKTEKIYKINRTLLEKSQIAARAKKRLELKEFLLENPQPGRLTALNKQFSSPVVNFFREEGIIEVIEKE
ASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDQIGKEQNKPFLLEGITGSGKTEVYLHIIDNVLKLGKTAIVLVPEISLT
PQMTNRFISRFGKQVAIMHSGLSEGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQESNPRYHAR
DVALLRAEYYKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIPQVEIIDFRNFIGQQEVSNFTSYLLDKIRDR
LDKKEQVVLMLNRRGYSSFIMCRDCGYVDQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKA
YEELLKVIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRSSERT
FQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEAFYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSY
EVLSLLKQGFSDKVKLLGPTPKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDMTQDKENRDLRLAIDHEPQNMM

Nucleotide


Download         Length: 2391 bp        

>NTDB_id=926767 AB6W33_RS02185 WP_000101528.1 365522..367912(+) (priA) [Streptococcus agalactiae strain GBS-B4009]
ATGGCAAGAAAATTAGCACAGGTTATTGTTGATATTCCTCTGATGCAGACAGATAAACCATTTTCGTATGCCATTCCTAA
AGATTTGGAAGATTTGGTACAAGTAGGAGTACGCGTTCATGTTCCTTTTGGAAGAGGAAATCGTCTTTTGCAAGGCTTTG
TTGTCGGTTTCAGAGATGATGATGAATTAGAGACTAAGGATATTGCGGAGGTCCTAGATTTTGAACCTGTTTTAAATCAG
GAACAACTAGACTTAGCTGATCAGATGCGCCATACTGTCTTTTCTTATAAAATATCTATTTTGAAATCAATGTTACCAAG
TCTTTTGAATTCGCAATATGATAAACTCTTGTTAGCAACAGACACATTACCATCAGAAGATAGAGAAGATCTATTTGGTC
ATAAAACGGAAATTGTCTTTTCAAGTTTGTCTTCTCAAGATGCCAAAAAAGCAGGCAGATTAATTCAAAAAGGTTTCATA
GAAGTTCAATATTTAGCCAAAGATAAGAAAACAATAAAAACAGAAAAGATTTACAAAATCAACAGAACTCTGTTAGAAAA
GAGCCAAATAGCAGCAAGAGCGAAAAAGAGACTGGAGTTGAAAGAATTCCTGTTAGAAAATCCTCAACCAGGGCGCCTCA
CAGCTTTAAACAAACAATTTTCATCTCCTGTGGTTAATTTTTTCAGAGAAGAAGGAATCATTGAAGTCATTGAAAAAGAA
GCTAGTAGATCTGATAATTATTTTAAAGGAATCCTAAAAACGGATTTTTTGGATTTAAATCAGGAACAAGCTAAAGTAGT
TAAAATAGTTGTTGACCAAATTGGTAAGGAACAGAATAAACCCTTCTTGTTGGAGGGGATTACAGGGTCTGGTAAGACAG
AGGTATATCTTCATATCATTGATAACGTTTTAAAGTTGGGAAAAACAGCGATTGTTCTCGTTCCAGAAATTTCTTTAACT
CCCCAGATGACGAATCGTTTTATTTCTCGTTTTGGAAAGCAAGTTGCTATTATGCACTCGGGCTTGTCAGAAGGTGAAAA
ATTTGATGAGTGGCGAAAGATAAAATCAGGTCAAGCTAAAGTTGTCGTTGGAGCTCGTTCAGCAATTTTTGCTCCTTTGG
AAAATATCGGAGCCATCATTATAGATGAGGAACATGAATCAACTTATAAGCAAGAATCTAACCCAAGGTATCATGCGCGC
GATGTGGCCTTGCTACGTGCAGAATATTATAAAGCTGTGTTACTAATGGGATCAGCAACTCCAAGTATCGAAAGCCGAGC
TAGAGCTAGTCGGGATGTTTATAAATTTTTAGAATTAAAACATCGTGCTAATCCTAAAGCACGTATTCCTCAGGTTGAAA
TTATTGATTTTAGAAATTTTATTGGTCAACAAGAAGTTAGTAATTTCACATCATATTTACTAGATAAAATCCGAGATAGA
TTAGATAAGAAAGAGCAAGTGGTTCTTATGCTCAATCGTAGAGGTTATTCTAGTTTTATTATGTGTCGAGACTGCGGCTA
CGTTGATCAATGTCCTAATTGTGACATTTCTCTGACTTTACATATGGCTACAAAGACCATGAATTGTCATTATTGTGGGT
TTGAAAAGCCTATTCCAAGAACATGTCCAAATTGCAATAGTAAAAGTATCTCTTACTATGGTACTGGAACCCAAAAAGCT
TATGAAGAACTTTTGAAAGTTATCCCAGACGCAAAGATTTTACGGATGGATGTTGATACGACAAGGCAAAAAGGCGGTCA
TGAGTCTATTTTGAAGCGTTTCGGAAATCATGAAGCTGATATTTTATTGGGAACACAGATGATTGCTAAGGGATTGGATT
TTCCAAATGTTACTTTAGTTGGAGTACTAAATGCAGATACTTCTCTGAATTTACCTGATTTTCGATCATCAGAGCGAACT
TTTCAGCTATTAACTCAAGTGGCTGGACGTGCTGGGCGTGCTGAAAAAGAAGGAGAGGTTGTCATTCAAACTTATAATCC
TAATCATTATGCTATTCAATTAGCCCAAAAGCAAGATTTTGAAGCTTTTTATCAATATGAAATGAATATCCGTCGACAAC
TTGGTTATCCTCCTTATTATTTTACAGTTGGTTTAACATTATCGCATAAAGATGAAGAGTGGTTAATACGAAAATCTTAT
GAAGTATTAAGTTTATTGAAGCAAGGCTTTAGTGATAAAGTAAAGTTGTTAGGTCCAACGCCAAAACCAATTGCAAGAAC
ACATAACCTTTATCACTACCAAATTATTATCAAGTATCGATTTGAGGACAACCTTGAACTTGTTTTGAATCGCTTATTAG
ACATGACTCAAGACAAGGAGAATAGAGATCTTCGCTTAGCAATAGACCACGAACCGCAAAATATGATGTAG

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(698-792)

Type III restriction enzyme, res subunit (ResIII)

(258-422)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-106)

Helicase conserved C-terminal domain (Helicase_C)

(571-654)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(511-537)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E1N9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Bacillus subtilis subsp. subtilis str. 168

46.906

100

0.476