Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JE76HU_RS20235 Genome accession   NZ_AP028094
Coordinates   3962822..3963289 (+) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain JNE132847     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3957822..3968289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JE76HU_RS20190 (O76HUS_38510) rplP 3957932..3958342 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  JE76HU_RS20195 (O76HUS_38520) rpsC 3958355..3959056 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  JE76HU_RS20200 (O76HUS_38530) rplV 3959074..3959406 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  JE76HU_RS20205 (O76HUS_38540) rpsS 3959421..3959699 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  JE76HU_RS20210 (O76HUS_38550) rplB 3959716..3960537 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  JE76HU_RS20215 (O76HUS_38560) rplW 3960555..3960857 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  JE76HU_RS20220 (O76HUS_38570) rplD 3960854..3961459 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  JE76HU_RS20225 (O76HUS_38580) rplC 3961470..3962099 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  JE76HU_RS20230 (O76HUS_38590) rpsJ 3962132..3962443 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  JE76HU_RS20235 (O76HUS_38600) pilD 3962822..3963289 (+) 468 WP_000339836.1 A24 family peptidase Machinery gene
  JE76HU_RS20240 (O76HUS_38610) bfr 3963286..3963762 (-) 477 WP_000675515.1 bacterioferritin -
  JE76HU_RS20245 (O76HUS_38620) bfd 3963835..3964029 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  JE76HU_RS20250 (O76HUS_38630) tuf 3964212..3965396 (-) 1185 WP_000031783.1 elongation factor Tu -
  JE76HU_RS20255 (O76HUS_38640) fusA 3965467..3967581 (-) 2115 WP_000124700.1 elongation factor G -
  JE76HU_RS20260 (O76HUS_38650) rpsG 3967678..3968148 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=92484 JE76HU_RS20235 WP_000339836.1 3962822..3963289(+) (pilD) [Escherichia coli strain JNE132847]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=92484 JE76HU_RS20235 WP_000339836.1 3962822..3963289(+) (pilD) [Escherichia coli strain JNE132847]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361

Multiple sequence alignment