Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   VML59_RS20185 Genome accession   NZ_CP142003
Coordinates   3887397..3889736 (-) Length   779 a.a.
NCBI ID   WP_074567759.1    Uniprot ID   A0A1Y5ZEC9
Organism   Bacillus pacificus strain AT31     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3882397..3894736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VML59_RS20155 - 3882716..3882892 (-) 177 WP_001242605.1 hypothetical protein -
  VML59_RS20160 - 3883010..3883159 (-) 150 WP_000271075.1 hypothetical protein -
  VML59_RS20165 - 3883159..3883854 (-) 696 WP_000447394.1 alpha/beta fold hydrolase -
  VML59_RS20170 - 3883985..3884425 (-) 441 WP_001266956.1 D-tyrosyl-tRNA(Tyr) deacylase -
  VML59_RS20175 relA 3884443..3886626 (-) 2184 WP_001262814.1 GTP diphosphokinase -
  VML59_RS20180 - 3886837..3887349 (-) 513 WP_000346214.1 adenine phosphoribosyltransferase -
  VML59_RS20185 recJ 3887397..3889736 (-) 2340 WP_074567759.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  VML59_RS20190 - 3889838..3890731 (-) 894 WP_074567762.1 cation diffusion facilitator family transporter -
  VML59_RS20195 secDF 3891038..3893302 (-) 2265 WP_016718528.1 protein translocase subunit SecDF -
  VML59_RS20200 - 3893573..3893857 (-) 285 WP_000886980.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88063.57 Da        Isoelectric Point: 4.8686

>NTDB_id=920824 VML59_RS20185 WP_074567759.1 3887397..3889736(-) (recJ) [Bacillus pacificus strain AT31]
MLQPKTRWKEKEYNSERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYIALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIAFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVETNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADTILTEEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMSLRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKGKITMVYFSEEVLNKFSLEDYKEHMMHASEVTELDDQYIVLLDLPKGTDELRE
LFKVGFPSRIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESSTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=920824 VML59_RS20185 WP_074567759.1 3887397..3889736(-) (recJ) [Bacillus pacificus strain AT31]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATAGCGAGCGAGTGAGTGAATTAGCAAGTAAATTACAATT
ATCACCACTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCGTTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACAACTGTTTTATATATTGCCTTACAAGAATT
AGGTGCAGACGTAGAATTTTATATCCCAAACCGTTTTACAGAAGGTTATGGACCGAATGAAGAAGCATTTCGTTGGGCGC
ATAGCGCAGGATTTTCACTCATTATTACTGTCGATACTGGTATCGCAGCTGTACATGAAGCGAAGGTAGCGAAGGAACTG
GGGATAGATCTAATTATTACAGATCACCATGAGCCACCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTGTATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCACATGCACTACTGGGCCGTG
TGCCAGAACATTTATTAGAAATTGCAGTAATTGGTACGGTAGCCGATTTAGTATCACTTCACGGTGAGAATAGATTGCTA
GTGAAGCGCGGTTTAAAACATATGCGTATGACGAAAAATATCGGACTAAAGGCGCTATTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGCCTTCTCAATCGCACCTCGTATTAATGCAGTTGGACGTTTAGAAGATGCAACGC
CGGCTGTACATCTTTTATTATCAGAGGATCCCGAAGAAGCGAAAGAGCTGGCTGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTGGAAACGAATTTCCCTCCTGAAGACAATAAAGT
ATTAGTGCTTGCAAAAGAAGGATGGAATCCAGGTGTAATTGGAATTGTCGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTATGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCGCGTAGTATTGCAGGATTTGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGTTATTGCCACACTTCGGAGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGTTTAAATGAACAGGCAGATACAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CTGTCGATGCTTTTTGCAAAGTGGAGGACGTAACACTTGCAGCAATTGAAGATATGCAAAAGTTAGCGCCATTTGGTGTT
GGAAATCCAAAGCCACGTATTGCAGTGAAAGATGCGGAGTTAGAAAGTATTCGTGCAATCGGATCAGATGGTTCTCATTT
AAAGATGTCTCTTCGTGATGGACAAGCAACATTGGATACAATTGGATTCGGTTTTGGTGCGTACGCGAAAGAAATTTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCGTCTATCAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTACAA
GATATTGCTGTAGAGGCATGGCAATTATTCGATTGGCGTAGTATGCGTAACGTAGAAGCAAATTTAGCAGAACTTCCGAA
AGGGAAAATCACGATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTGACCGAGTTAGATGATCAATACATCGTATTACTTGATTTACCAAAAGGGACAGATGAATTAAGAGAG
CTATTTAAAGTAGGATTCCCGTCGCGAATTTATACACTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGAGAACATTTTAAATGGTACTATTCGTTCTTGAGCCAAAAATCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCAACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGATAAAAAACAAAAGCGTGATTTAATTGAATCGAGCACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y5ZEC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.842

99.358

0.525