Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VKI21_RS05455 Genome accession   NZ_CP141255
Coordinates   1234288..1235403 (-) Length   371 a.a.
NCBI ID   WP_099435874.1    Uniprot ID   -
Organism   Cyanobacterium aponinum UTEX 3222     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1229288..1240403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VKI21_RS05440 (VKI21_05440) - 1230348..1232576 (-) 2229 WP_324283118.1 type II and III secretion system protein -
  VKI21_RS05445 (VKI21_05445) - 1232670..1233425 (-) 756 WP_324281452.1 type II and III secretion system protein -
  VKI21_RS05450 (VKI21_05450) - 1233422..1234285 (-) 864 WP_099435875.1 PilN domain-containing protein -
  VKI21_RS05455 (VKI21_05455) pilM 1234288..1235403 (-) 1116 WP_099435874.1 type IV pilus assembly protein PilM Machinery gene
  VKI21_RS05460 (VKI21_05460) - 1235692..1237023 (+) 1332 WP_342597587.1 sugar ABC transporter substrate-binding protein -
  VKI21_RS05465 (VKI21_05465) - 1237175..1237630 (-) 456 WP_206602558.1 hypothetical protein -
  VKI21_RS05470 (VKI21_05470) nrtS 1237629..1237859 (+) 231 WP_342597588.1 nitrate/nitrite transporter NrtS -
  VKI21_RS05475 (VKI21_05475) - 1238203..1238928 (+) 726 WP_324281455.1 class I SAM-dependent methyltransferase -
  VKI21_RS05480 (VKI21_05480) - 1239191..1239340 (+) 150 WP_071880491.1 DNA-binding protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41194.46 Da        Isoelectric Point: 4.2942

>NTDB_id=916424 VKI21_RS05455 WP_099435874.1 1234288..1235403(-) (pilM) [Cyanobacterium aponinum UTEX 3222]
MLSFLDNFMGGKTNGVGIEISPDKVTLAQISKKGQQYKLLKYYSLEVPEEIFEEGQIVDSPALAELIDQLVKEAKVKPKQ
IATAVPMKDAVMRIIPIPAELNDQELRDMVLNHEAALYLPYPREEVDIDYQKLGYFVDEDGIEKVQVLLVATRREVTDLY
LETFQQAGLKVSVLEINSFALIRTIKEQLKQFSSSEAVVLVDIEFDTTEIAIICEGVPQFTRTIPIGTYQMQTALAQAMN
LPTSKNTELLQEITIPNNPEQTTSASTTSSQTWINPGMDSLLRVLGELVDELRRSINFYLNQSGDVEVVQMLLSGPGAGL
AQIDEFFTKKLSLPTMQIDPISALSLSLKEDLSPMQRAGLGTVLGLGMRMS

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=916424 VKI21_RS05455 WP_099435874.1 1234288..1235403(-) (pilM) [Cyanobacterium aponinum UTEX 3222]
ATGTTAAGTTTTTTAGACAACTTCATGGGGGGGAAAACCAACGGTGTCGGTATTGAAATCAGTCCCGATAAAGTCACCCT
TGCTCAAATTAGTAAAAAAGGTCAACAATACAAATTACTCAAGTACTATTCTTTAGAAGTGCCAGAAGAAATATTTGAGG
AAGGACAAATAGTTGATTCTCCGGCATTGGCTGAGTTGATTGACCAACTTGTAAAGGAGGCAAAGGTTAAACCAAAGCAA
ATTGCTACTGCCGTACCCATGAAAGATGCGGTTATGCGTATTATACCAATTCCTGCAGAATTAAATGATCAGGAATTAAG
AGACATGGTGCTTAACCATGAAGCCGCCTTATATCTACCTTATCCTAGAGAAGAAGTGGACATAGACTATCAAAAATTAG
GCTACTTTGTGGATGAGGATGGCATCGAAAAAGTACAAGTTTTGTTAGTTGCTACCAGAAGAGAAGTAACTGACTTATAC
CTAGAAACATTCCAACAGGCAGGATTGAAAGTAAGTGTTCTGGAAATCAATAGTTTTGCTTTAATTAGAACTATAAAAGA
ACAACTCAAACAATTTTCTTCCAGTGAGGCGGTGGTTTTAGTGGATATTGAGTTTGATACTACCGAAATTGCCATTATCT
GTGAAGGTGTACCCCAATTTACCCGCACAATCCCCATTGGCACATATCAAATGCAAACAGCCTTGGCTCAAGCAATGAAT
TTGCCCACTTCCAAAAACACAGAACTATTGCAGGAAATCACTATTCCCAATAATCCAGAACAAACCACCAGCGCTAGTAC
CACTAGCAGTCAAACTTGGATAAACCCCGGCATGGATTCTTTATTAAGGGTTTTAGGTGAATTAGTGGATGAGTTGCGTC
GTTCTATCAATTTCTATCTCAATCAGAGTGGAGATGTGGAAGTAGTGCAGATGCTTTTATCTGGCCCGGGGGCGGGTTTG
GCACAAATTGATGAGTTTTTTACTAAAAAATTAAGTTTACCAACGATGCAAATTGACCCTATTTCGGCTTTATCTCTGAG
CTTGAAAGAGGATTTATCACCGATGCAAAGGGCTGGATTGGGTACTGTACTTGGTTTAGGAATGAGGATGAGCTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

65.405

99.73

0.652