Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   TM2_RS12330 Genome accession   NZ_AP025262
Coordinates   2391807..2394140 (-) Length   777 a.a.
NCBI ID   WP_236809981.1    Uniprot ID   -
Organism   Bacillus altitudinis strain TM2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2386807..2399140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TM2_RS12315 (TM2_23960) dtd 2388456..2388899 (-) 444 WP_048003401.1 D-aminoacyl-tRNA deacylase -
  TM2_RS12320 (TM2_23970) - 2388918..2391128 (-) 2211 WP_008342726.1 RelA/SpoT family protein -
  TM2_RS12325 (TM2_23980) - 2391271..2391783 (-) 513 WP_008342729.1 adenine phosphoribosyltransferase -
  TM2_RS12330 (TM2_23990) recJ 2391807..2394140 (-) 2334 WP_236809981.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  TM2_RS12335 (TM2_24000) - 2394222..2394578 (-) 357 WP_017367707.1 LapA family protein -
  TM2_RS12340 (TM2_24010) - 2394678..2395166 (-) 489 WP_008342734.1 cation:proton antiporter regulatory subunit -
  TM2_RS12345 (TM2_24020) secDF 2395367..2397595 (-) 2229 WP_017358118.1 protein translocase subunit SecDF -
  TM2_RS12350 (TM2_24030) - 2397637..2397933 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 777 a.a.        Molecular weight: 87600.37 Da        Isoelectric Point: 4.8290

>NTDB_id=90773 TM2_RS12330 WP_236809981.1 2391807..2394140(-) (recJ) [Bacillus altitudinis strain TM2]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVQRGLEEVEEARSFLFDQKAEFHDPFLLKGMKVAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVELLDLAAIGTIADLVPLHDENRWI
AKKGLLQLRQSRRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDMDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGADQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLQADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDEMTVQAIEEVGLLSPFGMQN
PKPFIMIEDAQLEDIRTIGANQNHIKMSLKDEDKLLDCVGFHQGKLKEELVPGSQISVVGEVSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDTAKTMVFCFDEETKRQSELVDIPIHLIDAENVSTFDVQEKYVIFADVPADEHLLKL
LLENQQPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIE
NGVLSIVRDAPKKDLTASSSYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2334 bp        

>NTDB_id=90773 TM2_RS12330 WP_236809981.1 2391807..2394140(-) (recJ) [Bacillus altitudinis strain TM2]
ATGTTATTATCCAAAATGCGTTGGGAATATGAACATCCTAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCACTTCTTGTACAAAGAGGGCTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCTTATTAAAAGGAATGAAAGTAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGATTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACACTTAAAGAACG
ATCTGCGAAGGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGATATGGGCCAAACGAACAGGCTTTCCGATATATCA
AAGAGCAAGGAGCATCGCTTGTCATCACCGTGGATACAGGGATCGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGATTAGACCTGATCATCACAGATCACCATGAACCAAGCGAGGAACTGCCTGAGGCACTTGCGATTGTACATCCTAAGCA
GCCTGGGTGTGAGTATCCGTTTAAAGAGCTGGCGGGTGTCGGTGTTGCGTTTAAAGTGGCACATGCACTCCTCGGTAAAC
TCCCAGTAGAATTATTAGATCTTGCTGCAATCGGTACAATTGCGGATCTTGTCCCGCTGCATGATGAAAATAGATGGATT
GCCAAAAAAGGTCTTCTGCAGCTTCGACAGTCTAGACGGCCTGGGTTAAAAGCGTTATTAAAAGAAGCAGGCGCCACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCCCCAAGGCTGAATGCTGTTGGACGAATTGAGCAGGCAGATC
CGGCTGTTCATCTTCTCATGACTGAGGATATGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAATTGAACAAAGAA
CGGCAGAAAATTGTGAGTACGATCACGGAAGAAGCCATTCAAATGGTCGAAGAGACAGGTGCTGACCAGTCTGCTATCGT
CGTAGCAAAAGCTGGCTGGAATCCAGGCGTTGTCGGCATTGTTGCCTCAAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGACGAAGAAACGCAGATGGCGAAAGGGTCAGCTCGAAGTATTCCTGGCTTTGATTTATTTTTCCAT
TTGAGCAAGTGCCGTGACATCCTGCCGCATTTCGGTGGACACCCGATGGCAGCAGGAATGACACTTCAAGCGGATGATGT
TCCTTTACTTAGGGAAAGATTGAATCAATATGCAAATGAAACATTGACAGAAGAAGATTTCATTCCAATTCAACGGGTCG
ATGCAGTATGTAAAGTGGACGAAATGACGGTGCAAGCCATTGAAGAAGTGGGACTGCTGTCCCCGTTTGGCATGCAGAAT
CCTAAGCCGTTCATTATGATTGAAGACGCCCAGCTTGAAGACATCCGAACGATCGGGGCCAATCAAAATCACATTAAAAT
GTCACTAAAAGACGAAGACAAGCTGCTCGACTGTGTCGGTTTTCACCAAGGCAAGCTAAAAGAAGAACTTGTCCCAGGGA
GTCAAATTTCTGTCGTTGGTGAGGTCTCGATTAATGAATGGAATAACCGAAAGAAACCTCAGCTCATGCTAAAAGACGCC
AGAGTCGATGAATGGCAGCTCTTTGATTTACGCGGGAAAAAAGATTGGGAAAAGAAAGTATCAACACTTGATACAGCAAA
AACGATGGTCTTCTGTTTTGACGAAGAAACAAAAAGGCAATCGGAGCTGGTGGATATTCCGATTCACCTTATTGATGCAG
AAAATGTATCCACGTTTGATGTGCAGGAAAAGTATGTGATCTTTGCCGATGTTCCTGCAGATGAACACTTGCTCAAGCTG
CTTTTAGAAAATCAACAACCAGCAAGAATTTATACGGTGTTTCAGCGGAAAGAAGATCACTTTATGTCCTCATTCCCGAG
CCGTGATCAATTCAAATGGTTCTATGGGTTCTTGTTCAAACGGGGAAGTTTTCCTATTAAAGAACAAGGCATGGAGCTTG
CCAAACATAGAGGTTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAG
AATGGTGTGCTGTCAATAGTGCGTGATGCCCCTAAAAAAGATTTAACCGCATCATCGTCTTATACAGCAAAACAACGATT
GATGGAACTCGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCT
CACCTGTGCTTTGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-766)

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(314-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.181

100

0.672

Multiple sequence alignment