Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   ABLU27_RS13240 Genome accession   NZ_CP157500
Coordinates   2561582..2562361 (-) Length   259 a.a.
NCBI ID   WP_050428201.1    Uniprot ID   -
Organism   Lactococcus lactis strain 2B-9     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2556582..2567361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLU27_RS13225 (ABLU27_13200) - 2558082..2559026 (-) 945 WP_406834640.1 hypothetical protein -
  ABLU27_RS13230 (ABLU27_13205) - 2559349..2560443 (-) 1095 WP_406834641.1 hypothetical protein -
  ABLU27_RS13235 (ABLU27_13210) - 2560454..2561398 (-) 945 WP_406834642.1 hypothetical protein -
  ABLU27_RS13240 (ABLU27_13215) endA 2561582..2562361 (-) 780 WP_050428201.1 DNA/RNA non-specific endonuclease Machinery gene
  ABLU27_RS13245 (ABLU27_13220) - 2562532..2563428 (-) 897 WP_406834643.1 peptidoglycan amidohydrolase family protein -
  ABLU27_RS13250 (ABLU27_13225) - 2563540..2563794 (-) 255 WP_406834644.1 phage holin -
  ABLU27_RS13255 (ABLU27_13230) - 2563784..2564086 (-) 303 WP_282798750.1 hypothetical protein -
  ABLU27_RS13260 (ABLU27_13235) - 2564300..2564593 (+) 294 WP_406834645.1 hypothetical protein -
  ABLU27_RS13265 (ABLU27_13240) - 2564623..2566242 (-) 1620 WP_406834646.1 glycoside hydrolase domain-containing protein -
  ABLU27_RS13270 (ABLU27_13245) - 2566342..2566689 (-) 348 WP_406834647.1 DUF722 domain-containing protein -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29129.05 Da        Isoelectric Point: 9.0583

>NTDB_id=906099 ABLU27_RS13240 WP_050428201.1 2561582..2562361(-) (endA) [Lactococcus lactis strain 2B-9]
MKNKKIIPLLIVSIFILGGCANSQSDMDKSNHKWSQKDKTPSEELAKSVLTSSIKEQLKTDNIKFDHGSFIVNNNNNDLA
IINVVDPYAKNEIDIQGRPYLGEAVLPKSIQNNEKDNKDEKVIPQFGWGQKSNLKGFYKKAYTKGFLLNSKLIGGVKDFN
DSGSNMENIFTQTVWASEANDSSSKGQKYFENMIHKVQDKNMIIKYRVKALYDGSNLVPSGTEIEAKSDDFSLEIHVFIP
NVQPNLKVDYKTGQVTEVK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=906099 ABLU27_RS13240 WP_050428201.1 2561582..2562361(-) (endA) [Lactococcus lactis strain 2B-9]
ATGAAGAACAAAAAAATTATCCCATTACTGATTGTCAGCATTTTTATCTTAGGGGGCTGTGCTAACAGTCAATCTGATAT
GGATAAAAGTAATCATAAGTGGAGTCAAAAAGATAAGACACCCTCAGAAGAACTCGCAAAAAGTGTTCTGACTTCTTCAA
TAAAAGAACAATTAAAAACAGATAACATAAAATTTGATCACGGTTCATTTATCGTTAATAATAATAACAATGATTTAGCA
ATTATTAATGTTGTAGACCCATACGCTAAGAATGAAATAGATATTCAAGGGCGTCCTTATTTGGGTGAAGCAGTTCTCCC
AAAATCTATTCAAAATAATGAAAAGGATAATAAAGATGAAAAAGTGATTCCTCAATTTGGTTGGGGACAAAAATCTAACT
TGAAAGGCTTCTATAAAAAAGCTTATACTAAAGGTTTTTTGCTAAATAGCAAACTAATAGGTGGCGTTAAAGATTTCAAT
GATAGCGGGTCAAATATGGAAAATATATTTACTCAAACGGTTTGGGCGAGTGAAGCTAATGATTCAAGTTCCAAGGGACA
AAAATATTTTGAAAATATGATTCATAAAGTTCAAGATAAGAATATGATCATCAAGTATAGAGTGAAAGCACTCTATGATG
GAAGTAACCTTGTCCCATCAGGAACTGAAATAGAAGCAAAATCTGATGATTTTTCACTTGAGATACATGTTTTTATTCCA
AATGTTCAACCAAATCTTAAAGTAGATTATAAAACAGGTCAGGTCACTGAAGTTAAATAA

Domains

Predicted by InterProScan.

DNA/RNA non-specific endonuclease (Endonuclea_NS_2)

(92-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

42.174

88.803

0.375
  endA Streptococcus pneumoniae D39

42.174

88.803

0.375
  endA Streptococcus pneumoniae R6

42.174

88.803

0.375
  endA Streptococcus pneumoniae TIGR4

42.174

88.803

0.375