Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   SGW58_RS02430 Genome accession   NZ_CP138666
Coordinates   457483..458553 (+) Length   356 a.a.
NCBI ID   WP_000776412.1    Uniprot ID   -
Organism   Staphylococcus aureus strain DA70300     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452483..463553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGW58_RS02400 - 452906..454369 (+) 1464 WP_338435212.1 rhomboid family intramembrane serine protease -
  SGW58_RS02405 - 454350..454553 (+) 204 WP_000087561.1 YqgQ family protein -
  SGW58_RS02410 - 454550..455536 (+) 987 WP_000161314.1 ROK family glucokinase -
  SGW58_RS02415 - 455536..455865 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SGW58_RS02420 - 455862..456485 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SGW58_RS02425 comGA 456537..457511 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  SGW58_RS02430 comGB 457483..458553 (+) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  SGW58_RS02435 comGC 458567..458878 (+) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  SGW58_RS02440 comGD 458856..459302 (+) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  SGW58_RS02445 comGE 459289..459588 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  SGW58_RS02450 comGF 459506..460003 (+) 498 WP_001794055.1 competence type IV pilus minor pilin ComGF Machinery gene
  SGW58_RS02455 - 460100..460246 (+) 147 WP_001789879.1 hypothetical protein -
  SGW58_RS02460 - 460236..460760 (+) 525 WP_001015125.1 shikimate kinase -
  SGW58_RS02465 gcvT 460919..462010 (+) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  SGW58_RS02470 gcvPA 462030..463376 (+) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41429.85 Da        Isoelectric Point: 10.1030

>NTDB_id=904333 SGW58_RS02430 WP_000776412.1 457483..458553(+) (comGB) [Staphylococcus aureus strain DA70300]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNKILSLIG
YSDTIVLQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=904333 SGW58_RS02430 WP_000776412.1 457483..458553(+) (comGB) [Staphylococcus aureus strain DA70300]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATAAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCCTGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
GATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994