Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   RGF10_RS06545 Genome accession   NZ_CP137761
Coordinates   1281168..1283534 (+) Length   788 a.a.
NCBI ID   WP_318508254.1    Uniprot ID   -
Organism   Bacillus sp. T3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1276168..1288534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGF10_RS06530 spoVB 1276622..1278184 (-) 1563 WP_318508250.1 stage V sporulation protein B -
  RGF10_RS06535 - 1278312..1278608 (+) 297 WP_318508252.1 post-transcriptional regulator -
  RGF10_RS06540 secDF 1278760..1281029 (+) 2270 Protein_1251 protein translocase subunit SecDF -
  RGF10_RS06545 recJ 1281168..1283534 (+) 2367 WP_318508254.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  RGF10_RS06550 - 1283525..1284038 (+) 514 Protein_1253 adenine phosphoribosyltransferase -
  RGF10_RS06555 - 1284360..1286556 (+) 2197 Protein_1254 RelA/SpoT family protein -
  RGF10_RS06560 dtd 1286572..1287012 (+) 441 WP_318508256.1 D-aminoacyl-tRNA deacylase -

Sequence

Protein


Download         Length: 788 a.a.        Molecular weight: 88797.46 Da        Isoelectric Point: 5.1242

>NTDB_id=900562 RGF10_RS06545 WP_318508254.1 1281168..1283534(+) (recJ) [Bacillus sp. T3]
MLRSKSRWIVRQSNEEKIQQLQQELAISPLVASLLINRGIETVEDAKSFLHTNDQSFHDPYLLKDMDKAVTRIKQAIEQG
EQIFIFGDYDADGVSSTTVMMKTLQDLGANVQYYIPNRFTEGYGPNEPAFRYASECGVSLIITVDTGISALHEAQVAKEL
GIDLIITDHHEPGPVLPEALAIIHPKLEDSIYPFRELAGVGVAFKLAHALYGSLPEHLLDIVIIGTIADLVSLTGENRLI
TKLGIRKMKSTTNVGLQALLKLAGVNSNSINEETVGFVLGPRINAAGRLESADIAVDLLLTEDPEEANSIAEEMDQLNKQ
RQALVNSITEEAIKEVEDNFPISENSVLVIGKEGWNAGVIGIVASKLVDRFYRPTIVLSFDREKGLAKGSARSIAGFDLF
QNLSTCREILPHFGGHPMAAGMTLKIDDVAELRGRLNDLAKEQLTEEDFIPIVKIDSAVALRDIDLSSIEEMQMLSPYGV
DNPKPKVLINEVALSTIRKIGSEQNHLKVTLEESTAKLDGVGFGLGHLYDHISPNAKLSVIGELAINEWNNIRKPQIFLS
DLAIRSWQLFDYRGQKRQNQFLDSLPIQQRKWVVFNEKNKAIIPDYQNQDVLWIESIDYAKNVNIDQQHIVLVDLPPTKE
MVQHLLQGKQPARIYVQFYKKDSDFFSTIPTREHFKWFYAFLAKKGPFNMKQHGHLLAKQRGWTNETIEFMSQVFFELDF
VKINNGLITLVPSAPKRDLTESTTYQMKLAQTSLENDLLYSSYQQLKNWFDQMIQGFVNHEEEVKEWI

Nucleotide


Download         Length: 2367 bp        

>NTDB_id=900562 RGF10_RS06545 WP_318508254.1 1281168..1283534(+) (recJ) [Bacillus sp. T3]
ATGTTACGATCAAAATCAAGATGGATAGTTCGTCAGTCAAATGAAGAGAAAATTCAACAGCTTCAGCAAGAGCTTGCTAT
TTCTCCGCTTGTTGCATCATTACTCATCAATCGCGGCATAGAAACGGTCGAGGATGCAAAAAGCTTCTTACACACGAACG
ATCAATCCTTTCATGACCCCTATTTACTGAAAGATATGGATAAGGCTGTCACCAGGATTAAGCAGGCGATTGAGCAGGGT
GAACAGATTTTCATCTTTGGAGATTACGACGCAGATGGCGTAAGTAGTACTACCGTCATGATGAAAACGCTCCAGGACCT
AGGTGCCAACGTACAGTACTACATTCCAAACCGCTTTACAGAAGGTTATGGTCCAAATGAGCCGGCTTTTCGCTATGCAT
CAGAATGTGGTGTTTCATTAATTATTACGGTAGATACTGGTATTTCTGCCTTACACGAAGCCCAGGTAGCAAAAGAATTA
GGCATTGATTTAATTATTACCGATCATCATGAGCCAGGCCCAGTCTTGCCAGAGGCATTGGCGATCATTCATCCGAAATT
AGAGGATAGTATTTATCCTTTCCGTGAATTAGCGGGTGTTGGTGTAGCATTTAAATTAGCCCATGCACTCTATGGTTCTT
TACCAGAGCATTTACTAGATATTGTCATAATCGGCACGATTGCTGATCTTGTTTCCCTAACAGGGGAGAATCGGTTAATA
ACCAAACTCGGCATACGTAAAATGAAATCAACAACAAATGTTGGCTTGCAGGCGTTATTAAAGCTTGCCGGAGTTAATTC
TAATAGTATTAATGAAGAGACAGTAGGCTTTGTATTAGGTCCGCGTATTAATGCGGCCGGACGCTTAGAAAGTGCTGATA
TAGCTGTCGATTTGCTTTTAACTGAAGATCCAGAAGAAGCAAATAGTATTGCTGAAGAAATGGACCAGCTAAATAAACAA
AGGCAGGCACTTGTTAATTCCATTACGGAAGAAGCTATTAAAGAGGTCGAGGATAACTTTCCAATCTCTGAGAACTCAGT
ATTGGTCATTGGAAAAGAAGGATGGAACGCTGGCGTTATTGGGATTGTTGCTTCAAAGTTAGTCGATCGCTTTTATCGGC
CGACGATTGTCCTTAGTTTTGATAGAGAAAAAGGCTTAGCGAAGGGCTCTGCTCGAAGCATTGCCGGTTTTGACTTGTTT
CAAAATCTCTCAACCTGTCGCGAAATTCTTCCTCATTTCGGTGGCCATCCAATGGCTGCAGGGATGACCTTGAAGATCGA
TGATGTTGCAGAGTTAAGAGGACGGCTGAATGATTTAGCAAAAGAGCAATTGACAGAGGAAGATTTTATTCCAATTGTTA
AAATTGATAGTGCTGTCGCCTTAAGGGATATCGATTTGTCTTCGATCGAAGAAATGCAGATGCTCTCTCCTTATGGGGTG
GATAATCCAAAGCCAAAGGTGTTAATCAATGAGGTAGCTCTATCGACGATTCGAAAAATAGGCTCAGAGCAAAATCATTT
AAAGGTAACGCTCGAAGAATCAACAGCTAAGCTTGATGGTGTTGGATTTGGCTTAGGTCATCTATATGACCACATTTCTC
CGAATGCTAAGCTCTCAGTAATAGGAGAGTTGGCGATAAATGAATGGAATAATATTCGTAAGCCGCAAATCTTTCTTTCT
GATTTAGCAATCCGCTCCTGGCAGCTGTTTGATTACCGAGGACAAAAACGTCAGAATCAATTTTTAGATTCACTTCCAAT
TCAGCAACGAAAATGGGTAGTTTTTAATGAGAAAAATAAAGCGATCATACCGGATTATCAAAATCAGGATGTTTTGTGGA
TTGAATCGATTGATTATGCAAAAAATGTTAACATCGATCAGCAGCACATTGTATTAGTAGATTTGCCGCCAACAAAAGAA
ATGGTGCAGCATTTGCTCCAAGGTAAGCAGCCAGCTAGAATCTACGTGCAATTTTATAAAAAAGATAGTGATTTCTTTAG
TACCATTCCAACTCGAGAACACTTTAAATGGTTTTATGCTTTCCTTGCTAAAAAAGGACCATTCAATATGAAACAGCATG
GTCATCTATTAGCAAAACAACGTGGCTGGACAAATGAAACAATTGAATTTATGTCACAGGTGTTTTTTGAATTGGACTTT
GTTAAAATAAACAATGGACTCATTACACTAGTGCCTAGTGCACCGAAACGAGACTTAACAGAATCAACGACGTATCAGAT
GAAGTTAGCGCAAACCTCCCTTGAAAATGATTTGCTGTATTCATCTTATCAGCAGTTAAAAAATTGGTTTGATCAAATGA
TTCAAGGGTTTGTTAATCATGAGGAGGAAGTAAAAGAATGGATTTGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(314-438)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

54.768

98.477

0.539