Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   ABFB49_RS23215 Genome accession   NZ_CP155726
Coordinates   5013368..5013793 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 2024CK-00357     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5008368..5018793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFB49_RS23200 (ABFB49_23200) pilX 5008932..5009519 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  ABFB49_RS23205 (ABFB49_23205) pilY1 5009531..5013022 (+) 3492 WP_003145982.1 type 4a pilus biogenesis protein PilY1 -
  ABFB49_RS23210 (ABFB49_23210) pilY2 5013024..5013371 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  ABFB49_RS23215 (ABFB49_23215) comF 5013368..5013793 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  ABFB49_RS23220 (ABFB49_23220) ispH 5013840..5014784 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ABFB49_RS23225 (ABFB49_23225) fkpB 5014870..5015310 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  ABFB49_RS23230 (ABFB49_23230) lspA 5015303..5015812 (-) 510 WP_003102615.1 signal peptidase II -
  ABFB49_RS23235 (ABFB49_23235) ileS 5015805..5018636 (-) 2832 WP_347175678.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=897953 ABFB49_RS23215 WP_003094721.1 5013368..5013793(+) (comF) [Pseudomonas aeruginosa strain 2024CK-00357]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=897953 ABFB49_RS23215 WP_003094721.1 5013368..5013793(+) (comF) [Pseudomonas aeruginosa strain 2024CK-00357]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383