Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   R5H20_RS06375 Genome accession   NZ_CP137345
Coordinates   1274097..1277597 (+) Length   1166 a.a.
NCBI ID   WP_006636759.1    Uniprot ID   M5PF09
Organism   Bacillus sp. KICET-3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1269097..1282597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5H20_RS06350 (R5H20_06350) - 1269752..1271119 (-) 1368 WP_006636754.1 sensor histidine kinase -
  R5H20_RS06355 (R5H20_06355) - 1271100..1271759 (-) 660 WP_051056661.1 response regulator transcription factor -
  R5H20_RS06360 (R5H20_06360) - 1271907..1272347 (-) 441 WP_006636756.1 ferritin-like domain-containing protein -
  R5H20_RS06365 (R5H20_06365) modA 1272531..1273322 (+) 792 WP_006636757.1 molybdate ABC transporter substrate-binding protein -
  R5H20_RS06370 (R5H20_06370) modB 1273331..1273987 (+) 657 WP_006636758.1 molybdate ABC transporter permease subunit -
  R5H20_RS06375 (R5H20_06375) addB 1274097..1277597 (+) 3501 WP_006636759.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  R5H20_RS06380 (R5H20_06380) addA 1277584..1281273 (+) 3690 WP_006636760.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  R5H20_RS06385 (R5H20_06385) sbcD 1281343..1282527 (+) 1185 WP_006636761.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134004.87 Da        Isoelectric Point: 5.5038

>NTDB_id=897569 R5H20_RS06375 WP_006636759.1 1274097..1277597(+) (addB) [Bacillus sp. KICET-3]
MGAVFLSGRSGSGKTTFILDDIREKLRHDPLGKPIIFLVPDQMTFLMEYELSKTPDLGGTIRAQVYSFSRLAWRILQRTG
GMNRPFLTGTGIQMLLRKLIEEHKDEFKVYQNASDKSGFTEQVERMLTEFKRHCLTPDSIREIMQGSGTSEYEDERILSD
KLHDLYILYSKMEESLKDQYVQSEDYLTLLAEQIPHAEEIREASIYVDGFHQFTPQEMLVLEQLMAHAPEITFSLTADRP
FTGNIPHELHLFRMTGKAYFDLHQKARELGLDVSEIRLEEAKRHKHAPELQHVERYFDERPANAYTGKIESFRIMQAANR
RTEIEGIAREIRSLVRSGAFRLKDIAVIARNLEDYQDAIKEVFTDYGLAFFIDGKESMRNHPLIELIRSTLDIIKGNWRY
EAVFRCIKTELLFPGDKPKERVREQVDQLENYCIAYGIQGNRWTSSEPFVYRRYTSLDEDFAQTDREIETENMLNELKDW
IVPPVHRLQKRLKKAGSVREMAEAVYLYLEEADIPLKLEEERRIAEESGRIEESRQHEQVWDAVIQLLDEFVEMMGDERI
SFSLFQQMIETGLESLTFSLIPPALDQVFIGNMDLSRMYGIKCTFLIGANDGILPARPADDGVLTDDDREWLKRNGAALA
ATGREQLLDENYLIYMALSSPSHQLYVSYPIADAEGKTLLPSTIVKRLNELFPDHAEKMLANEPEQLDDEAQLEFLVNKS
TALSYLAGQLGIWSRQYAISDVWWSTYNFLMDEPDRRFSQNVLSSLFFRNKVHTLNQRVSRELYGEHIQGSVSRMEMYKA
CPFSHFASHGLKLKERQFFKLEAPDIGQLFHSALKLISDRLHELKLDWRDLTKEQCDALSSDAVERLAPKLQKEILLSSN
RHHYVKQKLQKIIARVSGILSEHAKASGFAPVGLELGFGGKGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKASPAGVLYFHVHDPMIQASAPLGLDEIEKEIFKKFKMKGL
LLGDQEAIKLMDTTLEQGRSDIISAGLKKDGSLRSDSAVVREEDFHLLRHHIRRTFQKAGEEITDGKVAIEPYKLKDRTP
CTFCSFKSFCQFDESLEENEYRVLKPEKDNVILDWLKKEDEANGDG

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=897569 R5H20_RS06375 WP_006636759.1 1274097..1277597(+) (addB) [Bacillus sp. KICET-3]
GTGGGAGCAGTTTTTTTATCAGGCAGGTCCGGGAGTGGGAAAACGACCTTTATCTTGGATGACATCAGAGAAAAGCTTCG
CCATGACCCGCTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAGATGACGTTTTTAATGGAATATGAGCTTAGTAAAA
CTCCTGATTTGGGCGGGACGATCCGCGCCCAGGTTTACAGTTTTTCAAGGCTTGCCTGGCGAATCCTCCAGCGCACGGGA
GGCATGAACCGTCCGTTTCTGACGGGAACCGGCATTCAAATGCTGCTTCGCAAATTGATCGAAGAGCATAAGGATGAGTT
TAAAGTGTATCAAAACGCGAGTGACAAAAGCGGTTTTACGGAGCAGGTTGAACGAATGCTGACCGAGTTTAAACGGCACT
GCCTGACGCCAGATAGCATTCGCGAGATTATGCAGGGAAGCGGGACGTCGGAATATGAGGATGAACGGATTCTTTCCGAC
AAGCTCCACGACCTCTATATTTTGTACAGCAAGATGGAAGAAAGCCTCAAAGATCAATATGTTCAGTCAGAAGATTATTT
GACACTCTTGGCGGAACAGATACCGCATGCCGAAGAGATCAGGGAAGCTTCCATTTATGTGGACGGTTTTCACCAATTCA
CCCCGCAGGAGATGCTCGTCCTTGAACAGCTGATGGCACATGCGCCGGAGATTACATTTTCTTTGACGGCGGATCGGCCC
TTTACAGGAAACATACCGCATGAATTGCATCTTTTCCGGATGACGGGAAAGGCATATTTTGATCTTCACCAAAAAGCACG
GGAGCTCGGCCTTGATGTGTCAGAAATCCGCCTGGAAGAAGCGAAACGGCATAAGCACGCGCCGGAGCTGCAACATGTGG
AGCGTTATTTTGATGAAAGGCCGGCAAACGCGTACACAGGAAAAATAGAGTCTTTCCGCATCATGCAGGCGGCTAACAGA
AGAACCGAGATTGAAGGGATCGCCAGGGAAATCCGCTCCCTGGTCCGCAGCGGCGCGTTCAGGCTGAAAGATATCGCGGT
GATCGCCCGCAATCTTGAAGATTATCAAGACGCGATCAAAGAAGTGTTTACAGATTACGGACTTGCGTTTTTCATAGACG
GCAAAGAGTCCATGCGGAACCATCCGCTGATCGAACTGATTCGTTCAACACTTGATATCATTAAAGGAAACTGGAGATAT
GAAGCGGTATTCCGCTGCATCAAAACAGAGCTTTTATTCCCCGGGGACAAGCCGAAGGAACGGGTGCGGGAACAGGTAGA
CCAGCTAGAAAACTATTGCATTGCTTATGGTATTCAAGGGAACAGGTGGACAAGCAGTGAACCGTTTGTTTACAGAAGAT
ATACTTCATTGGATGAGGACTTTGCACAGACCGACCGGGAAATTGAGACTGAGAACATGCTCAATGAATTAAAGGACTGG
ATTGTGCCGCCTGTTCACCGCCTGCAAAAACGCCTGAAAAAAGCGGGATCGGTGAGGGAAATGGCTGAAGCCGTTTATCT
CTATCTTGAAGAGGCCGATATTCCGCTGAAGCTTGAAGAGGAAAGGCGGATTGCCGAAGAAAGCGGCCGGATCGAGGAAT
CAAGACAGCATGAACAGGTCTGGGACGCCGTCATTCAATTATTGGACGAGTTTGTGGAAATGATGGGGGACGAGCGCATA
TCGTTTTCCTTGTTTCAGCAAATGATCGAAACCGGTTTGGAGTCGTTAACATTCTCGCTGATTCCGCCTGCGCTTGATCA
GGTGTTTATCGGCAATATGGATTTATCCAGAATGTACGGTATCAAATGCACGTTTCTCATCGGGGCAAACGACGGCATCC
TGCCGGCAAGGCCGGCCGATGACGGGGTTTTGACAGATGACGACAGGGAGTGGCTGAAAAGAAACGGGGCGGCGCTTGCG
GCAACGGGAAGAGAACAGCTGCTTGATGAAAATTATTTGATCTACATGGCGCTCTCAAGCCCTTCTCATCAGCTCTATGT
CTCATATCCGATCGCAGATGCTGAAGGGAAAACGCTGCTTCCCTCAACTATTGTGAAGCGGTTAAACGAACTGTTTCCGG
ATCATGCCGAGAAGATGCTCGCCAACGAACCGGAACAGCTGGATGATGAGGCACAGCTTGAATTTCTCGTCAATAAAAGC
ACTGCTTTAAGCTATTTGGCGGGGCAGCTCGGGATCTGGTCGAGACAGTACGCGATCAGCGATGTATGGTGGAGTACATA
CAACTTTTTGATGGATGAGCCTGATCGGCGATTTTCACAAAATGTCCTGTCGAGCTTGTTTTTCCGAAACAAAGTTCACA
CTCTCAATCAGCGGGTAAGCCGGGAACTGTACGGGGAGCACATCCAGGGGAGCGTGTCGCGGATGGAAATGTACAAAGCC
TGTCCGTTTTCCCACTTTGCTTCCCATGGCTTAAAGCTGAAGGAACGCCAATTTTTCAAACTTGAAGCTCCGGATATCGG
CCAGCTGTTTCATTCAGCGCTGAAGCTGATCTCAGACCGTCTTCATGAACTGAAGCTCGATTGGCGCGACTTGACGAAAG
AACAATGTGATGCACTATCAAGCGATGCGGTAGAACGGCTCGCGCCGAAACTGCAAAAAGAGATTTTGCTCAGCTCAAAC
CGTCACCACTATGTGAAGCAAAAGCTGCAAAAAATTATCGCGCGGGTATCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGGTTTGCTCCCGTCGGTCTCGAGCTTGGCTTCGGCGGAAAAGGTCCGCTTCCTCCGATGCGGTTTACTCTTAAAAACG
GCTGCACAATGGAGCTTGTCGGCCGGATTGACCGGGTGGATAAAGCCGAGAGCTCAAAAGGGCTGCTGCTGCGAATCGTG
GATTACAAATCAAGCGATAAAGGCCTTGATCTGGCTGAAGTGTATTACGGCCTTGCGCTGCAAATGCTGACATATCTTGA
TTTGTCCATCACCCATTCGGCGGATTGGCTCGGGATGAAGGCCTCGCCGGCCGGAGTTCTTTATTTTCATGTGCACGATC
CGATGATTCAGGCCTCCGCACCGCTCGGCCTGGATGAGATTGAAAAAGAAATATTTAAGAAATTTAAGATGAAAGGGCTT
CTGCTGGGAGACCAGGAAGCCATCAAGCTGATGGACACCACCCTTGAACAGGGCAGGTCCGACATCATCAGCGCAGGCTT
GAAAAAAGACGGTTCGCTCCGTTCGGATTCTGCGGTCGTCAGAGAAGAAGATTTTCACTTATTAAGACACCACATCCGCC
GGACGTTTCAAAAAGCCGGCGAGGAGATTACAGACGGTAAAGTGGCGATCGAGCCTTATAAATTAAAAGACAGGACCCCG
TGCACATTTTGTTCTTTCAAATCATTCTGCCAGTTTGACGAATCGCTTGAGGAGAATGAGTACCGGGTATTGAAGCCGGA
AAAAGACAATGTGATATTGGATTGGCTGAAAAAGGAGGATGAGGCAAATGGAGATGGCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M5PF09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

73.947

99.743

0.738