Detailed information NTG0896076

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: LLNCDO184_RS03565
• Genome accession: NZ_CP155124
• Coordinates: 640026..642365 (+)
• Length: 779 a.a.
• NCBI ID: WP_003131470.1
• Uniprot ID: Q9CEE5
• Organism: Lactococcus lactis subsp. lactis strain NCDO184
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 635026..647365

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLNCDO184_RS03545 (LLNCDO184_03535)	-	636050..636763 (+)	714	WP_003131466.1	glucosamine-6-phosphate deaminase	-
LLNCDO184_RS03550 (LLNCDO184_03540)	rny	636994..638589 (+)	1596	WP_003131467.1	ribonuclease Y	-
LLNCDO184_RS03555 (LLNCDO184_03545)	gmk	638737..639354 (+)	618	WP_003131468.1	guanylate kinase	-
LLNCDO184_RS03560 (LLNCDO184_03550)	rpoZ	639534..639887 (+)	354	WP_010906189.1	DNA-directed RNA polymerase subunit omega	-
LLNCDO184_RS03565 (LLNCDO184_03555)	priA	640026..642365 (+)	2340	WP_003131470.1	primosomal protein N'	Machinery gene
LLNCDO184_RS03570 (LLNCDO184_03560)	-	642424..642798 (+)	375	WP_014570768.1	nuclear transport factor 2 family protein	-
LLNCDO184_RS03575 (LLNCDO184_03565)	-	642811..643333 (+)	523	Protein_680	NUDIX hydrolase	-
LLNCDO184_RS03580 (LLNCDO184_03570)	-	643299..643763 (+)	465	WP_003131474.1	VOC family protein	-
LLNCDO184_RS03585 (LLNCDO184_03575)	fmt	643760..644719 (+)	960	WP_010906187.1	methionyl-tRNA formyltransferase	-
LLNCDO184_RS03590 (LLNCDO184_03580)	-	644894..645136 (+)	243	WP_003131476.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
LLNCDO184_RS03595 (LLNCDO184_03585)	amaP	645164..645724 (+)	561	WP_010906186.1	alkaline shock response membrane anchor protein AmaP	-
LLNCDO184_RS03600 (LLNCDO184_03590)	-	645735..645923 (+)	189	WP_012898445.1	DUF2273 domain-containing protein	-
LLNCDO184_RS03605 (LLNCDO184_03595)	-	645944..646450 (+)	507	WP_003131479.1	Asp23/Gls24 family envelope stress response protein	-

Sequence

Protein

• Length: 779 a.a.
• Molecular weight: 88929.26 Da
• Isoelectric Point: 6.5580

>NTDB_id=896076 LLNCDO184_RS03565 WP_003131470.1 640026..642365(+) (priA) [Lactococcus lactis subsp. lactis strain NCDO184]
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENK
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVRSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Nucleotide

• Length: 2340 bp

>NTDB_id=896076 LLNCDO184_RS03565 WP_003131470.1 640026..642365(+) (priA) [Lactococcus lactis subsp. lactis strain NCDO184]
ATGAAAATTGCTAAAGTCATCGTTGATGTGCCTCTGATGCAGACGGATAAACCCTTTTCTTACAGCCTTTCAGCTGAATT
TGAAAATTTGCTTGAAGTGGGCATGCGGGTCCATGTTCCTTTTGGAAGTGGAAACCGGCTTGTTCAAGGAATTGTCATTG
GATTTGAAGATGAAGAACTTGAGCCGTCAGTAAATTTGAAAGGAATTTCTGAACTACTCGACGTTGAGCCAGTCCTAACG
GATGAACAGCTTTGGTTGGCAGATGAAATGCGTCATTCAGTATTTTCTTATAAAATTTCATGTCTAAAAGCCATGTTACC
TAATCTTTTGAATTCTAATTATGACAAGCTCCTAAAATCGACTGACGGAACTTCTGTCAAATGGTCACAACTGACAGAAG
CAGAAAAAATCTCGTCAGCAAAGGCTGTGCGTTTGGGTCAGCTGTCAGTAGAATATTTAGCAAAATCTAAAGAAAATAAA
AAAACAGAAAAATTCTTATCTATTCCAGATATCGAAAAACTAGCTGAATTTGAAATTGAAAAGAGAGCTAAAAAACGCTT
AGAGTTGAAATATTTTCTATTAAGACTTCAAGAAGAAAAAATTGCTGTCAGATCATTAAATTTTTCTTCACAAATCATTA
AATTTTTCGTTGAGCATGACCTAGTCAAAATTGAAGAAGTTGAAATTTCGAGGACGAAAGAAAAATTTGAAGATGTGGAA
AATGACAGTGCTAAAATTCTCAACTCTGAACAAAATGCAGCTTACGAAAAAATAATTGCATCTTCTGACAAACCATTTTT
GTTGGAAGGAATTACTGGTTCAGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTCAAACTCGGTAAAACAG
CAATTATGCTTGTGCCAGAAATTTCTTTGACGCCACAGATTACTAATCGATTTATTGCTCGTTTTGGAGATAAAGTGGCG
ATTATGCATTCTGGTTTATCTGATGGTGAAAAATATGATGAATGGCGAAAAATTAAATCTGGCAAAGCTCAGGTAGTTGT
CGGTGCACGTTCAGCTATTTTTGCACCTGTTGAAAATTTGGGAATTATCATCATTGATGAAGAACATGAAACTTCATATA
AGCAAGATTCAAGTCCCCGTTATCATGCAAGAGATATTGCGCTTTTGCGTGCAAAATGGCATGGTGCAAGACTTGTTTTA
GGTTCTGCAACGCCAAGTTTAGAAACGCGAGCACGTGCCATGCGCGGAGTTTATGAACTTTTATTACTGACAAAAAGGGC
AAATGAAGCAGCAATCCTGCCAGAAATAAAAATCATTGATATGCGCAGTGAAATTTCTGACCAATCTGCTAATTTTTCAC
AATCTTTACTGACAAAAATAAAAGAAAAAATTGACAAAGATGAACAAGTCATTTTGATGTTGAATCGAAGGGGTTATTCT
TCATTTGTCATGTGTCGTGAATGTGGTTTTGTTGTTGATTGTCCCAATTGCGATATTTCCTTGACCCTTCACATGGATAC
CCGAACTTTGAATTGTCATTATTGTGGTCATGTCGAAGGAATTCCGCAAACTTGTCCTAATTGTCATTCACGAAAAATTC
GTTATTACGGTTCAGGAACGCAAAAAGTCGAAGAAGAACTTCGAGAAATCATGCCTGAAGCACGAGTTTTGCGAATGGAT
GTAGATACGACTCGGACAAAAAATGCTCACGAAAAAATTCTTGAAAAGTTTGGTAATCATGAAGCTGATATCCTCTTAGG
AACTCAGATGATTGCCAAAGGTTTAGATTTTCCAAACGTAACCTTAGTGGGTGTCATTAACGCAGATACTGGCCTAAATC
TACCTGATTTTAGAGCGAGTGAGCGTTCCTTTGAATTGTTGATGCAGGTGGCTGGACGAGCTGGACGAGCAGATAAAAAA
GGTGAAGTATTAATTCAAACTTTCAATCCACATCATTATGCTATTGAAATGACCAAGGCTCAAGATTACGAAAAATTTTA
TGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACCGTTCAAGTGCTTGTTTCTCATCAAG
ATGAAGAAGTTGCCATTCAAAAATCTTATGAGATCGCTCAATTACTCAAGCAAAATTTGACAGAAAAAGCTAAAATATTA
GGGCCAACCGCCAGACCAATTGCCAGAACACATAACCTTTATCATTATCAAATCTTAGTTAAATATCGTTTTGAAGAAGA
ATTAGAATCAGCTTTAAATCAAGTGCTTGAATTAACCCAACTTCGGGATAACAAAAATTTACGAGTATTGATTGATAGTG
AGCCACAAAATTTTGTATAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (681-775)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-107)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (494-518)

• Helicase conserved C-terminal domain (Helicase_C) (561-637)

• Type III restriction enzyme, res subunit (ResIII) (247-405)

3D structure

• AlphaFold DB: Q9CEE5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.637	100	0.492