Detailed information NTG0894627

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: AAVC70_RS02685
• Genome accession: NZ_CP154898
• Coordinates: 360525..361025 (-)
• Length: 166 a.a.
• NCBI ID: WP_013352868.1
• Uniprot ID: A0A9P1JIL5
• Organism: Bacillus amyloliquefaciens strain Fad 97
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 355525..366025

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVC70_RS02640 (AAVC70_02640)	-	355644..356438 (+)	795	WP_013352859.1	YqhG family protein	-
AAVC70_RS02645 (AAVC70_02645)	sinI	356618..356791 (+)	174	WP_013352860.1	anti-repressor SinI family protein	Regulator
AAVC70_RS02650 (AAVC70_02650)	sinR	356825..357160 (+)	336	WP_014470659.1	transcriptional regulator SinR	Regulator
AAVC70_RS02655 (AAVC70_02655)	-	357208..357993 (-)	786	WP_013352862.1	TasA family protein	-
AAVC70_RS02660 (AAVC70_02660)	-	358058..358642 (-)	585	WP_013352863.1	signal peptidase I	-
AAVC70_RS02665 (AAVC70_02665)	tapA	358614..359285 (-)	672	WP_013352864.1	amyloid fiber anchoring/assembly protein TapA	-
AAVC70_RS02670 (AAVC70_02670)	-	359543..359872 (+)	330	WP_013352865.1	DUF3889 domain-containing protein	-
AAVC70_RS02675 (AAVC70_02675)	-	359913..360092 (-)	180	WP_013352866.1	YqzE family protein	-
AAVC70_RS02680 (AAVC70_02680)	comGG	360146..360523 (-)	378	WP_013352867.1	competence type IV pilus minor pilin ComGG	Machinery gene
AAVC70_RS02685 (AAVC70_02685)	comGF	360525..361025 (-)	501	WP_013352868.1	competence type IV pilus minor pilin ComGF	Machinery gene
AAVC70_RS02690 (AAVC70_02690)	comGE	360934..361248 (-)	315	WP_014470662.1	competence type IV pilus minor pilin ComGE	Machinery gene
AAVC70_RS02695 (AAVC70_02695)	comGD	361232..361669 (-)	438	WP_013352869.1	competence type IV pilus minor pilin ComGD	Machinery gene
AAVC70_RS02700 (AAVC70_02700)	comGC	361659..361967 (-)	309	WP_013352870.1	competence type IV pilus major pilin ComGC	Machinery gene
AAVC70_RS02705 (AAVC70_02705)	comGB	361972..363009 (-)	1038	WP_013352871.1	competence type IV pilus assembly protein ComGB	Machinery gene
AAVC70_RS02710 (AAVC70_02710)	comGA	362996..364066 (-)	1071	WP_013352872.1	competence type IV pilus ATPase ComGA	Machinery gene
AAVC70_RS02715 (AAVC70_02715)	-	364260..365210 (-)	951	WP_013352873.1	magnesium transporter CorA family protein	-

Sequence

Protein

• Length: 166 a.a.
• Molecular weight: 17813.77 Da
• Isoelectric Point: 10.2182

>NTDB_id=894627 AAVC70_RS02685 WP_013352868.1 360525..361025(-) (comGF) [Bacillus amyloliquefaciens strain Fad 97]
MVIITKSVQLSAAKKKCASAFSKQTGFTLLNVLVSLAVCLFLSGTLSPILHLFLSGRTDNGGIDSREHHIAVQQIMNECK
QAETVEPADGGRALTCRKTGGEEVRFEIYHTMIRKRVNGKGHVPILHNAASLTADVKNGLILVGITSVTGKKKQTAIPVY
SSLKGD

Nucleotide

• Length: 501 bp

>NTDB_id=894627 AAVC70_RS02685 WP_013352868.1 360525..361025(-) (comGF) [Bacillus amyloliquefaciens strain Fad 97]
ATGGTGATTATTACAAAGTCTGTGCAGCTGTCCGCGGCGAAAAAGAAATGTGCCTCAGCATTCTCAAAACAGACTGGCTT
TACGCTTCTTAATGTTTTGGTCTCCTTGGCCGTCTGTCTCTTCTTATCAGGCACTCTGAGCCCAATTTTACATTTGTTTT
TATCAGGCCGGACCGATAACGGAGGAATAGACAGCCGGGAGCATCATATTGCCGTTCAGCAGATCATGAATGAATGTAAA
CAGGCTGAGACTGTGGAGCCTGCAGATGGCGGACGGGCGTTAACCTGCAGGAAAACAGGAGGCGAAGAAGTGCGTTTTGA
AATCTATCACACGATGATAAGGAAAAGAGTGAACGGAAAAGGCCATGTGCCGATCCTGCATAACGCTGCTTCATTAACGG
CTGATGTGAAAAACGGCCTGATTTTAGTGGGAATCACAAGCGTTACAGGTAAAAAGAAGCAGACGGCCATTCCGGTTTAC
AGCTCTTTAAAAGGTGATTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (65-152)

3D structure

• AlphaFold DB: A0A9P1JIL5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Bacillus subtilis subsp. subtilis str. 168	47.619	75.904	0.361