Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   AAEU35_RS10240 Genome accession   NZ_CP152294
Coordinates   2009665..2010444 (+) Length   259 a.a.
NCBI ID   WP_050428201.1    Uniprot ID   -
Organism   Lactococcus lactis strain Q13     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2004665..2015444
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEU35_RS10210 - 2004702..2005049 (+) 348 WP_023189066.1 DUF722 domain-containing protein -
  AAEU35_RS10215 - 2005149..2007389 (+) 2241 WP_317141312.1 glycoside hydrolase domain-containing protein -
  AAEU35_RS10220 - 2007419..2007712 (-) 294 WP_025017162.1 hypothetical protein -
  AAEU35_RS10225 - 2007929..2008219 (+) 291 WP_124175219.1 hypothetical protein -
  AAEU35_RS10230 - 2008209..2008463 (+) 255 WP_023189062.1 phage holin -
  AAEU35_RS10235 - 2008569..2009471 (+) 903 WP_230593160.1 peptidoglycan amidohydrolase family protein -
  AAEU35_RS10240 endA 2009665..2010444 (+) 780 WP_050428201.1 DNA/RNA non-specific endonuclease Machinery gene
  AAEU35_RS10245 - 2010535..2010984 (-) 450 WP_058223250.1 GNAT family N-acetyltransferase -
  AAEU35_RS10250 - 2011133..2012248 (+) 1116 WP_010906150.1 AI-2E family transporter -
  AAEU35_RS10255 - 2012370..2013719 (+) 1350 WP_058223249.1 hemolysin family protein -
  AAEU35_RS10260 pflA 2013925..2014719 (+) 795 WP_003130318.1 pyruvate formate-lyase-activating protein -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29129.05 Da        Isoelectric Point: 9.0583

>NTDB_id=891849 AAEU35_RS10240 WP_050428201.1 2009665..2010444(+) (endA) [Lactococcus lactis strain Q13]
MKNKKIIPLLIVSIFILGGCANSQSDMDKSNHKWSQKDKTPSEELAKSVLTSSIKEQLKTDNIKFDHGSFIVNNNNNDLA
IINVVDPYAKNEIDIQGRPYLGEAVLPKSIQNNEKDNKDEKVIPQFGWGQKSNLKGFYKKAYTKGFLLNSKLIGGVKDFN
DSGSNMENIFTQTVWASEANDSSSKGQKYFENMIHKVQDKNMIIKYRVKALYDGSNLVPSGTEIEAKSDDFSLEIHVFIP
NVQPNLKVDYKTGQVTEVK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=891849 AAEU35_RS10240 WP_050428201.1 2009665..2010444(+) (endA) [Lactococcus lactis strain Q13]
ATGAAGAACAAAAAAATTATCCCATTACTGATTGTCAGCATTTTTATCTTAGGGGGCTGTGCTAACAGTCAATCTGATAT
GGATAAAAGTAATCATAAATGGAGTCAAAAAGATAAGACACCCTCAGAAGAACTCGCAAAAAGTGTTCTGACTTCTTCAA
TAAAAGAACAATTAAAAACAGATAACATAAAATTTGATCACGGTTCATTTATCGTTAATAATAATAACAATGATTTAGCA
ATTATTAATGTTGTAGACCCATACGCTAAGAATGAAATAGATATTCAAGGGCGTCCTTATTTGGGTGAAGCAGTTCTCCC
AAAATCTATTCAAAATAATGAAAAGGATAATAAAGATGAAAAAGTGATTCCTCAATTTGGTTGGGGACAAAAATCTAACT
TGAAAGGCTTCTATAAAAAAGCTTATACTAAAGGTTTTTTGCTAAATAGTAAACTAATAGGTGGCGTTAAAGATTTCAAT
GATAGCGGGTCAAATATGGAAAATATATTTACTCAAACGGTTTGGGCGAGTGAAGCTAATGATTCAAGTTCCAAGGGACA
AAAATATTTTGAAAATATGATTCATAAAGTTCAAGATAAGAATATGATCATCAAGTATAGAGTGAAAGCACTCTATGATG
GAAGTAACCTTGTCCCATCAGGAACTGAAATAGAAGCAAAATCTGATGATTTTTCACTCGAGATACATGTTTTTATTCCA
AATGTTCAACCAAATCTTAAAGTAGATTATAAAACAGGTCAGGTCACTGAAGTCAAATAA

Domains

Predicted by InterProScan.

DNA/RNA non-specific endonuclease (Endonuclea_NS_2)

(92-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

42.174

88.803

0.375
  endA Streptococcus pneumoniae D39

42.174

88.803

0.375
  endA Streptococcus pneumoniae R6

42.174

88.803

0.375
  endA Streptococcus pneumoniae TIGR4

42.174

88.803

0.375