Detailed information NTG0891844

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: AAEU35_RS09785
• Genome accession: NZ_CP152294
• Coordinates: 1922936..1925275 (+)
• Length: 779 a.a.
• NCBI ID: WP_023189130.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain Q13
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1917936..1930275

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAEU35_RS09765	-	1918960..1919673 (+)	714	WP_015427049.1	glucosamine-6-phosphate deaminase	-
AAEU35_RS09770	rny	1919904..1921499 (+)	1596	WP_023189132.1	ribonuclease Y	-
AAEU35_RS09775	gmk	1921647..1922264 (+)	618	WP_003131468.1	guanylate kinase	-
AAEU35_RS09780	rpoZ	1922444..1922797 (+)	354	WP_010906189.1	DNA-directed RNA polymerase subunit omega	-
AAEU35_RS09785	priA	1922936..1925275 (+)	2340	WP_023189130.1	primosomal protein N'	Machinery gene
AAEU35_RS09790	-	1925334..1925708 (+)	375	WP_025017158.1	nuclear transport factor 2 family protein	-
AAEU35_RS09795	-	1925721..1926242 (+)	522	Protein_1874	NUDIX hydrolase	-
AAEU35_RS09800	-	1926208..1926672 (+)	465	WP_012898447.1	VOC family protein	-
AAEU35_RS09805	fmt	1926669..1927628 (+)	960	WP_058223547.1	methionyl-tRNA formyltransferase	-
AAEU35_RS09810	-	1927803..1928045 (+)	243	WP_003131476.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
AAEU35_RS09815	amaP	1928073..1928633 (+)	561	WP_010906186.1	alkaline shock response membrane anchor protein AmaP	-
AAEU35_RS09820	-	1928644..1928832 (+)	189	WP_012898445.1	DUF2273 domain-containing protein	-
AAEU35_RS09825	-	1928853..1929359 (+)	507	WP_012898444.1	Asp23/Gls24 family envelope stress response protein	-

Sequence

Protein

• Length: 779 a.a.
• Molecular weight: 88945.26 Da
• Isoelectric Point: 6.5580

>NTDB_id=891844 AAEU35_RS09785 WP_023189130.1 1922936..1925275(+) (priA) [Lactococcus lactis strain Q13]
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENR
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVKSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNASYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Nucleotide

• Length: 2340 bp

>NTDB_id=891844 AAEU35_RS09785 WP_023189130.1 1922936..1925275(+) (priA) [Lactococcus lactis strain Q13]
ATGAAAATTGCTAAAGTCATCGTTGATGTGCCTCTGATGCAGACGGATAAACCCTTTTCTTACAGCCTTTCAGCTGAATT
TGAAAATTTGCTTGAAGTGGGCATGCGGGTTCATGTTCCTTTTGGAAGTGGAAACCGGCTTGTTCAAGGAATTGTCATTG
GATTTGAAGATGAAGAACTTGAGCCGTCAGTAAATTTGAAAGGAATTTCTGAACTACTCGACGTTGAGCCAGTCCTAACG
GATGAACAGCTTTGGTTGGCAGATGAAATGCGTCATTCAGTATTTTCTTATAAAATTTCATGTCTAAAAGCCATGTTACC
TAATCTTTTGAATTCTAATTATGACAAGCTCCTAAAATCGACTGACGGAACTTCTGTCAAATGGTCACAACTGACAGAAG
CAGAAAAAATCTCGTCAGCAAAGGCTGTGCGTTTGGGTCAGCTGTCAGTAGAATATTTAGCAAAATCTAAAGAAAATAGA
AAAACAGAAAAATTCTTATCTATTCCAGATATCGAAAAACTAGCTGAATTTGAAATTGAAAAGAGAGCCAAAAAACGCTT
AGAGTTGAAATATTTTCTATTAAGACTTCAAGAAGAAAAAATTGCAGTCAAATCATTAAATTTTTCTTCACAAATCATTA
AATTTTTCGTTGAGCATGACCTAGTCAAAATTGAAGAGGTTGAAATTTCGAGGACGAAAGAAAAATTTGAAGATGTGGAA
AATGACAGTGCTAAAATTCTCAACTCCGAACAAAATGCATCTTACGAAAAAATAATTGCATCTTCTGACAAACCATTTTT
GTTGGAAGGAATTACTGGTTCAGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTCAAACTCGGTAAAACAG
CAATTATGCTTGTGCCAGAAATTTCTTTGACGCCACAGATTACTAATCGATTTATTGCTCGTTTTGGAGATAAAGTGGCG
ATTATGCATTCTGGTTTATCTGATGGTGAAAAATATGATGAATGGCGAAAAATTAAATCTGGCAAAGCTCAGGTAGTTGT
CGGTGCACGTTCAGCTATTTTTGCACCTGTTGAAAATTTGGGAATTATCATCATTGATGAAGAACATGAAACTTCATATA
AGCAAGATTCAAGTCCCCGTTATCATGCAAGAGATATTGCGCTTTTGCGTGCAAAATGGCATGGTGCAAGACTTGTTTTA
GGTTCTGCAACGCCAAGTTTAGAAACGCGAGCACGTGCCATGCGCGGAGTTTATGAACTTTTATTACTGACAAAAAGGGC
AAATGAAGCAGCAATCCTGCCAGAAATAAAAATCATTGATATGCGCAGTGAAATTTCTGACCAATCTGCTAATTTTTCAC
AATCTTTACTGACAAAAATAAAAGAAAAAATTGACAAAGATGAACAAGTCATTTTGATGTTGAATCGAAGGGGTTATTCT
TCATTTGTCATGTGTCGTGAATGTGGTTTTGTTGTTGATTGTCCCAATTGCGATATTTCCTTGACCCTTCACATGGATAC
CCGAACTTTGAATTGTCATTATTGTGGTCATGTCGAAGGAATTCCGCAAACTTGTCCTAATTGTCATTCACGAAAAATTC
GTTATTACGGTTCAGGAACGCAAAAAGTCGAAGAAGAACTTCGAGAAATCATGCCTGAAGCACGAGTTTTGCGAATGGAT
GTAGATACGACTCGGACAAAAAATGCTCACGAAAAAATTCTTGAAAAGTTTGGTAATCATGAAGCTGATATCCTCTTAGG
AACTCAGATGATTGCCAAAGGTTTAGATTTTCCAAACGTAACCTTAGTGGGTGTCATTAACGCAGATACTGGCCTAAATC
TACCTGATTTTAGAGCGAGTGAGCGTTCCTTTGAATTGTTGATGCAGGTGGCTGGACGAGCTGGACGAGCAGATAAAAAA
GGTGAAGTATTAATTCAAACTTTCAATCCACATCATTATGCTATTGAAATGACCAAGGCTCAAGATTACGAAAAATTTTA
TGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACCGTTCAAGTGCTTGTTTCTCATCAAG
ATGAAGAAGTTGCCATTCAAAAATCTTATGAGATCGCTCAATTACTCAAGCAAAATTTGACAGAAAAAGCTAAAATATTA
GGGCCAACCGCCAGACCAATTGCCAGAACACATAACCTTTATCATTATCAAATCTTAGTTAAATATCGTTTTGAAGAAGA
ATTAGAATCAGCTTTAAATCAAGTGCTTGAATTAACCCAACTTCGGGATAACAAAAATTTACGAGTATTGATTGATAGTG
AGCCACAAAATTTTGTATAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-107)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (494-518)

• Helicase conserved C-terminal domain (Helicase_C) (561-637)

• Type III restriction enzyme, res subunit (ResIII) (245-405)

• Primosomal protein N C-terminal domain (PriA_C) (681-775)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.388	100	0.489