Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   RUL31_RS05900 Genome accession   NZ_CP135964
Coordinates   1212258..1215758 (+) Length   1166 a.a.
NCBI ID   WP_315916700.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain CD303     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1207258..1220758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RUL31_RS05880 (RUL31_05880) - 1208111..1209313 (-) 1203 WP_151291002.1 MFS transporter -
  RUL31_RS05885 (RUL31_05885) - 1209427..1211154 (+) 1728 WP_315916699.1 ABC transporter substrate-binding protein -
  RUL31_RS05890 (RUL31_05890) - 1211190..1211519 (-) 330 WP_106359801.1 four-helix bundle copper-binding protein -
  RUL31_RS05895 (RUL31_05895) - 1211615..1212052 (-) 438 WP_003327683.1 ferritin family protein -
  RUL31_RS05900 (RUL31_05900) addB 1212258..1215758 (+) 3501 WP_315916700.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  RUL31_RS05905 (RUL31_05905) addA 1215745..1219449 (+) 3705 WP_315916701.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  RUL31_RS05910 (RUL31_05910) sbcD 1219520..1220698 (+) 1179 WP_106359798.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134438.31 Da        Isoelectric Point: 5.1681

>NTDB_id=887801 RUL31_RS05900 WP_315916700.1 1212258..1215758(+) (addB) [Bacillus atrophaeus strain CD303]
MGAEFLVGRSGSGKTKLIIDSIQDELRRNPFGKPIIFLVPDQMTFLMEYELAKTPGIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTSTGIQMLLRKLIEEHKQEFKVYQKASDKSGFTEQVERMLTEFKRYCLEPEDIRRMIESGTATDYRGERVLTD
KLHDLYILYQQMEKSLENQYLHSEDYLTLLAEQIPYAEDLKGAHVYVDGFYQFTPQEFRVLTQLMAQAEHVTFSLTADKP
SHVQAPDELDLFRMTGKTYYQLHQAASELHLDITNRELTETKRYQQTPELAHLEAMYDARPAVPFLEKQEAFTVMQAANR
RTELEGIAREIHALVRDKGLRYKDMAILARQLEDYKDMVKEVFADYEIPYFIDGKASMLNHPLVEFIRSSLDVIKGNWRY
EAVFRCVKTELLFPLDRPKNIIREQVDQLENYCIAYGIKGDRWTRGDRFVYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFRLQKRMKQAKTVQDMAEALYLYLEETDIPLKLDQERQRAEEDGRIIEAQQHNQAWDAVIQLLEEFVEMMGEDEI
SLSLFQQMMETGAESLKFALIPPALDQVFVGNMDLSRMYGTRCTFVIGANDGVLPARPEDDGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSTLVKRLTELFPEHQERLLTNEPEQVSDCEQLLYLVNKS
VAQSYTASQFRLWLREYDISDVWWSTYNVLMNEPDRLNSKKLFSSLFFRNEVKPLERAVSRQLYGEHIHGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKDQCNKFSYEAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVARVSGILSEHAKASGFAPVGLELGFGGKGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLSEVYYGLALQMLTYLDLSITYSADWLGMKATPAGVLYFHIHDPMIQASVPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEEEFHLLTQHVRRTFQEAGEQITDGCVSIEPYKLKDKTP
CTYCSFKSVCQFDESLEENEYRTLKAEKDHTILDWLKKEGDGDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=887801 RUL31_RS05900 WP_315916700.1 1212258..1215758(+) (addB) [Bacillus atrophaeus strain CD303]
TTGGGAGCAGAGTTTTTAGTAGGCAGATCCGGGAGTGGAAAAACGAAGCTGATCATAGACAGCATTCAAGATGAGCTGCG
CCGGAATCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCTGGTATAGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGTTTGGCCTGGCGTGTCCTGCAGCATACGGGC
GGAATGAACCGGCCGTTTGTCACCAGCACGGGAATCCAAATGCTTTTGAGAAAGCTCATTGAAGAGCACAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGAACAGGTAGAACGGATGCTGACGGAATTTAAACGGTACT
GCCTTGAGCCTGAAGATATCCGCCGCATGATTGAAAGCGGAACTGCCACAGACTATCGCGGCGAGCGGGTGTTAACCGAT
AAGCTGCATGATTTGTATATTTTATATCAGCAGATGGAAAAGAGCCTTGAAAACCAGTATCTTCATTCAGAGGATTATTT
AACGCTGCTGGCGGAACAGATCCCGTATGCGGAAGATTTGAAAGGGGCGCATGTCTATGTAGACGGCTTTTATCAGTTTA
CTCCGCAGGAATTCAGAGTGCTTACGCAGCTTATGGCACAGGCTGAGCATGTGACGTTTTCACTGACGGCGGACAAGCCG
TCACATGTGCAGGCGCCTGATGAGCTGGATCTGTTCAGAATGACAGGCAAAACATATTATCAGCTCCATCAAGCAGCTTC
GGAGCTTCATTTGGACATCACAAACAGAGAACTGACCGAGACGAAACGATATCAGCAAACACCGGAGCTTGCCCATTTAG
AAGCAATGTATGACGCGCGCCCGGCTGTTCCGTTTTTGGAAAAGCAAGAAGCCTTTACCGTGATGCAGGCTGCGAACAGG
CGGACAGAGCTGGAAGGAATTGCCCGTGAAATTCATGCTCTTGTAAGAGATAAGGGATTACGCTACAAGGATATGGCAAT
TCTCGCCCGCCAGCTGGAAGATTATAAGGATATGGTTAAAGAAGTATTTGCAGATTATGAAATTCCTTACTTTATAGATG
GAAAGGCATCCATGCTGAACCATCCGCTTGTTGAGTTTATCCGTTCAAGCCTTGATGTCATAAAGGGAAATTGGAGATAT
GAAGCGGTGTTTCGCTGTGTGAAAACCGAACTGTTATTCCCGCTTGACCGTCCGAAGAACATCATAAGGGAGCAGGTCGA
TCAACTGGAAAACTATTGTATCGCTTACGGAATTAAAGGCGACCGATGGACGAGAGGCGACCGTTTTGTTTACAGGCGGT
TTGTTTCACTGGATGATGATTTTGCGCAGACAGATCAAGAGATTGAAATGGAAAACATGCTGAATGACACAAGAGACTGG
ATTGTTCCGCCGCTTTTCCGTCTTCAAAAACGGATGAAACAAGCAAAAACGGTCCAAGACATGGCGGAGGCTCTTTATCT
TTATTTAGAAGAAACGGATATTCCGTTAAAGCTGGACCAAGAAAGACAGCGCGCGGAAGAGGACGGGAGAATTATCGAAG
CACAGCAGCATAATCAGGCTTGGGACGCTGTCATACAGCTTCTCGAGGAATTTGTGGAAATGATGGGCGAAGACGAAATT
TCTCTGTCTCTTTTTCAGCAAATGATGGAGACGGGTGCGGAATCGTTAAAATTCGCTCTGATTCCGCCTGCACTCGATCA
AGTCTTTGTCGGCAATATGGATTTATCGAGAATGTACGGTACACGCTGCACCTTTGTTATCGGTGCGAACGACGGCGTAC
TTCCTGCGCGGCCTGAGGATGACGGGGTGCTGTCTGATGACGACCGGGAATGGCTGAAAACCATTGGAGTCGAGCTGTCA
TCCGGCGGGCGCGAACGTCTGCTTGATGAGCACTTTCTCATATATATGGCGCTTTCCAGTCCATCTGACCGCCTTTATGT
ATCCTACCCGATCGCAGACGCCGAAGGTAAAACGCTTTTGCCGTCAACTTTGGTCAAACGGCTTACAGAACTGTTTCCGG
AGCATCAGGAAAGGCTTTTGACCAATGAACCGGAACAGGTCAGCGACTGCGAACAGCTCTTGTATCTCGTCAATAAAAGC
GTGGCGCAATCGTATACGGCAAGCCAATTCAGGTTGTGGTTAAGGGAGTATGACATCAGTGATGTATGGTGGAGCACGTA
TAATGTATTGATGAATGAACCGGACAGGCTGAACTCGAAAAAACTTTTCTCCAGCCTGTTTTTCAGAAATGAAGTCAAAC
CGCTTGAACGCGCTGTATCGAGACAGCTGTACGGCGAGCATATTCATGGAAGTGTGTCCAGAATGGAGACCTTTAACGCT
TGTCCGTTCTCCCATTTTGCGTCCCACGGCTTACATCTGAAAGAACGGCAATTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTCCATTCCAGTCTGAAGCTGATTTCAGACCGGCTTCGTGAACAAAAGCTGGATTGGCGCGATTTAACAAAAG
ACCAATGTAACAAGTTCTCTTACGAGGCGGTGGAGCGGCTCGCGCCGAAGCTCCAAAAAGAAATTCTGCTTAGCTCAAAC
AGACATTATTATGTCAAGGAAAAGCTGCAAAAAATTGTGGCGCGCGTGTCCGGCATTTTAAGCGAGCATGCGAAGGCGAG
CGGGTTTGCACCGGTTGGGCTTGAGCTTGGTTTTGGCGGGAAAGGCCCGCTGCCGCCTCTTACATTCACATTAAAAAACG
GCTGCACGATGGAGCTTGTCGGACGCATTGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGGCTGCTGCTGAGAATAGTG
GATTATAAATCCAGCGACAAGGGTCTTGATTTGTCGGAGGTATATTACGGCCTTGCCCTGCAAATGCTCACGTACCTTGA
TTTATCCATTACGTATTCGGCAGATTGGCTCGGCATGAAGGCTACACCGGCCGGCGTTCTTTATTTCCATATTCACGACC
CGATGATTCAAGCCAGCGTTCCGCTCGGGCTTGATGAAATAGAGCAGGAAATTTTTAAGAAATTTAAGATGAAAGGCCTG
CTGCTCGGCGATCAGGAAGCGATTCGATTAATGGATACGACGCTCCAAGAGGGAAGGTCAAATATCATTAATGCCGGGCT
TAAAAAAGACGGTTCATTAAGGTCTGATTCAGCGGCGGTCGGAGAAGAGGAATTTCATCTGCTGACACAGCACGTGCGGC
GGACCTTTCAGGAGGCCGGCGAACAAATCACCGACGGGTGTGTGTCGATTGAGCCGTATAAGCTGAAAGATAAAACACCC
TGCACATACTGCTCATTCAAATCAGTATGCCAGTTCGATGAATCATTGGAAGAAAACGAATATCGCACGCTGAAAGCGGA
AAAAGATCATACCATATTAGATTGGCTGAAAAAGGAGGGAGATGGCGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

88.765

100

0.888