Detailed information NTG0887101

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: RS401_RS05795
• Genome accession: NZ_CP135601
• Coordinates: 1108106..1111831 (+)
• Length: 1241 a.a.
• NCBI ID: WP_047956369.1
• Uniprot ID: A0A0J1I2M7
• Organism: Bacillus sp. SI2
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1103106..1116831

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RS401_RS05780 (RS401_05780)	-	1103264..1103857 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
RS401_RS05785 (RS401_05785)	lepB	1103914..1104477 (+)	564	WP_000751894.1	signal peptidase I	-
RS401_RS05790 (RS401_05790)	addB	1104594..1108109 (+)	3516	WP_153576858.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
RS401_RS05795 (RS401_05795)	addA	1108106..1111831 (+)	3726	WP_047956369.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
RS401_RS05800 (RS401_05800)	-	1111844..1112131 (+)	288	WP_000718623.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
RS401_RS05805 (RS401_05805)	gerPF	1112169..1112384 (-)	216	WP_001141566.1	spore germination protein GerPF	-
RS401_RS05810 (RS401_05810)	-	1112427..1112813 (-)	387	WP_000902341.1	spore germination protein GerPE	-
RS401_RS05815 (RS401_05815)	gerPD	1112829..1113023 (-)	195	WP_001052802.1	spore germination protein GerPD	-
RS401_RS05820 (RS401_05820)	gerPC	1113030..1113644 (-)	615	WP_001070766.1	spore germination protein GerPC	-
RS401_RS05825 (RS401_05825)	gerPB	1113712..1113918 (-)	207	WP_001012512.1	spore germination protein GerPB	-
RS401_RS05830 (RS401_05830)	gerPA	1113933..1114154 (-)	222	WP_001111188.1	spore germination protein GerPA	-
RS401_RS05835 (RS401_05835)	-	1114251..1114430 (-)	180	WP_000462851.1	aspartyl-phosphate phosphatase Spo0E family protein	-
RS401_RS05840 (RS401_05840)	-	1114678..1115577 (+)	900	WP_038356517.1	fumarylacetoacetate hydrolase family protein	-
RS401_RS05845 (RS401_05845)	-	1115618..1115902 (-)	285	WP_001988577.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142622.88 Da
• Isoelectric Point: 4.8322

>NTDB_id=887101 RS401_RS05795 WP_047956369.1 1108106..1111831(+) (addA) [Bacillus sp. SI2]
MMENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSTFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHVRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIQTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAVEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGESGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
ETRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=887101 RS401_RS05795 WP_047956369.1 1108106..1111831(+) (addA) [Bacillus sp. SI2]
ATGATGGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGATCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCAGCAGCAGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAAAACAGAATT
GGAGAGGCTTTAGAAAAAGTATTAATTGATGAACCAGGATCTCAGCACGTAAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTCATTAGAGGATATTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAATGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCCCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACGGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACGCTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGCGATTATAACGAGGATATTGTCAAACAAGTAGACTCTCTTCGTAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAGCAAAGTGAAGATGGTGAAATGAAGCCGTCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGTTTCACACAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAGCAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCACAGCTTGAAGCACGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTACTTCGCTCGATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGACCTTGCCACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGCTCGTTTTATGAAGTAATGAGCACATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCACGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTCGCTGGACTTGGTCGTCGTTTTAATACGCAAGACTTAATGAA
ACGTTTCTTACTTCATAAAGACTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CACAACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGG
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCACGTACGTGCCGGAGCATCTTGTTATTTAGACTGGATTGCACCTTCCTTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAGTATTCCAGGTGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGGAACACGTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCTCTGCAAAGTGAACGAAAAGATGAAGTGTACGACAGGTTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAGGAAGGTAGCGATAACGCT
TTTATTAAAAAATTACGTGCACCAATTCAAACACGTCCTCGTTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGAGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTTGAACAAGCAGAAGAAATAGCAGTTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGGAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATTACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCGGGAGGATTCGAACAAGCGAAACCAATTTTA
GAAACTCGTTACAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

3D structure

• AlphaFold DB: A0A0J1I2M7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.666	100	0.537