Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   RGB74_RS06405 Genome accession   NZ_CP134049
Coordinates   1340042..1342393 (+) Length   783 a.a.
NCBI ID   WP_310762157.1    Uniprot ID   -
Organism   Bacillus sp. NEB1478     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1335042..1347393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGB74_RS06390 (RGB74_06390) spoVB 1335694..1337244 (-) 1551 WP_310762155.1 stage V sporulation protein B -
  RGB74_RS06395 (RGB74_06395) - 1337349..1337600 (+) 252 WP_310762156.1 post-transcriptional regulator -
  RGB74_RS06400 (RGB74_06400) secDF 1337753..1339970 (+) 2218 Protein_1274 protein translocase subunit SecDF -
  RGB74_RS06405 (RGB74_06405) recJ 1340042..1342393 (+) 2352 WP_310762157.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  RGB74_RS06410 (RGB74_06410) - 1342413..1342925 (+) 513 WP_310762158.1 adenine phosphoribosyltransferase -
  RGB74_RS06415 (RGB74_06415) - 1343081..1345264 (+) 2184 WP_310762159.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  RGB74_RS06420 (RGB74_06420) dtd 1345286..1345729 (+) 444 WP_310762160.1 D-aminoacyl-tRNA deacylase -
  RGB74_RS06425 (RGB74_06425) - 1345799..1345972 (+) 174 WP_310762161.1 hypothetical protein -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 87639.95 Da        Isoelectric Point: 4.8383

>NTDB_id=878367 RGB74_RS06405 WP_310762157.1 1340042..1342393(+) (recJ) [Bacillus sp. NEB1478]
MLKSRTRWKVQDASEEKAKHLAQELDLPLLIANLLLVRGIDSVEDAELFLNIDKNDFHDPFLLDGMKQSVDRIHKAIDSN
EKILIFGDYDADGVSSTTVMYYALKELGAQFDYYIPNRFTEGYGPNEPALRKAKEDGYSLVVTVDTGISAVHEANVAKEI
GLDFIITDHHEAPPILPEAYSIINPKKPGCTYPFKGLAGVGVAFKVAHALHGKPLVHLLDYAAIGTIADLVPLVDENRLI
AKLGIEALSKTDKIGLQELLKVAGLTDKDLSAEDVGFAVGPRVNAAGRLGSAMPVVELFTSTDREQSAMLAEEVDSVNKE
RQAIVNEITKEAIAMVEDSFPPEENEVLVLAKEGWNPGVIGIVASRLVEKFYRPTICLCLDPEKGTAKGSARSIEGFDMF
ENLSESRDILPHFGGHPMAAGMTLALQDVDELRKRLISQAKRILKPEDYVPVTSIDLTAKLDEISLDTVDLLNTIAPFGV
GNPSPRVLIEEVPIREMKRIGSQSNHLKLQVGGGELSVLDCIGFQKGDLIDEMTPYSQLSIVGQLQLNEWNGYRKPQLLL
EDISVPSFQLFDYRGIKDAGKRLLNLPYSKTNFITFQTQTLKELELESLSEHTLTADDINKLPDQAFENKFLVFLDVPSS
LSEFSVWLETKGEPDRIYAVFHQNSDHFFTTLPTREHFKWFYAFLAKRKTFNLKKDGNELAKWKGWSKDTIDFMSQVFFE
LEFVTIDDGVITINSKPGKRDLESSETYQKKFEQVDIENQLVYSSYHALKSWFTQQTRQKASL

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=878367 RGB74_RS06405 WP_310762157.1 1340042..1342393(+) (recJ) [Bacillus sp. NEB1478]
ATGTTGAAATCCAGAACGAGGTGGAAGGTACAAGACGCTTCAGAAGAAAAAGCTAAGCATCTTGCGCAGGAACTCGATCT
TCCACTATTAATTGCGAATCTTTTACTTGTTAGAGGAATTGACTCAGTTGAAGATGCTGAATTGTTTTTAAACATAGATA
AAAATGATTTTCATGACCCATTTTTATTGGATGGTATGAAGCAAAGCGTAGATCGAATACATAAGGCGATTGATTCGAAT
GAAAAGATCCTGATATTTGGTGACTATGATGCAGATGGTGTCAGCAGTACGACCGTTATGTACTATGCATTGAAAGAGCT
AGGTGCACAATTTGACTACTATATTCCAAATCGCTTTACTGAAGGCTATGGACCGAATGAACCGGCATTAAGAAAAGCGA
AAGAAGATGGGTATTCACTTGTCGTAACAGTAGATACAGGAATATCTGCTGTACATGAAGCCAATGTCGCTAAAGAAATC
GGGCTTGATTTTATTATTACGGACCACCACGAAGCACCGCCAATTTTACCGGAAGCTTATAGCATCATCAACCCCAAAAA
ACCAGGATGTACTTACCCTTTTAAAGGTCTAGCTGGAGTAGGTGTTGCATTTAAAGTGGCTCATGCACTTCATGGAAAAC
CATTAGTCCATCTCCTCGATTATGCCGCTATAGGGACGATAGCTGACCTTGTGCCGCTGGTTGATGAAAATAGGCTGATA
GCTAAGCTCGGCATTGAAGCTTTATCAAAAACAGATAAAATCGGTCTTCAGGAATTATTAAAAGTAGCCGGACTTACCGA
TAAGGACCTTTCAGCAGAAGATGTTGGGTTCGCTGTCGGTCCTCGTGTGAATGCTGCAGGAAGGCTTGGTTCAGCAATGC
CGGTTGTTGAATTATTCACATCAACCGACCGTGAGCAATCAGCAATGTTGGCTGAAGAAGTAGATTCTGTGAATAAAGAA
CGGCAAGCAATTGTGAACGAAATAACAAAAGAGGCCATTGCCATGGTAGAAGATTCTTTTCCGCCTGAAGAAAATGAAGT
TCTTGTTCTCGCAAAAGAAGGCTGGAATCCAGGAGTTATTGGAATTGTTGCATCTCGCTTAGTTGAAAAATTTTACCGCC
CAACGATTTGCTTATGTCTGGACCCTGAAAAAGGCACCGCAAAAGGATCTGCCCGCAGTATCGAAGGATTTGATATGTTT
GAGAATTTATCTGAATCCAGAGACATTCTCCCGCATTTTGGGGGACATCCAATGGCAGCTGGAATGACACTTGCACTTCA
AGATGTGGATGAGCTAAGAAAACGGCTGATTTCACAAGCAAAAAGGATATTGAAACCCGAAGATTACGTTCCAGTTACTT
CTATTGATTTGACTGCCAAGCTGGATGAAATCTCGCTAGATACAGTTGATCTTTTAAATACTATAGCTCCGTTTGGCGTA
GGAAATCCATCACCACGGGTTTTGATCGAAGAAGTTCCAATCAGGGAAATGAAGAGGATTGGCAGCCAATCAAACCACCT
AAAGCTGCAGGTAGGCGGAGGTGAGTTATCTGTTCTTGATTGTATCGGCTTCCAAAAGGGAGATCTCATTGATGAAATGA
CTCCATATTCTCAATTATCTATTGTTGGACAGCTGCAATTAAATGAATGGAATGGATACAGAAAGCCTCAGCTTTTACTT
GAAGACATATCTGTTCCTTCATTCCAGCTGTTTGATTACAGGGGAATAAAGGATGCAGGGAAAAGGCTGTTAAATCTGCC
TTACTCAAAAACGAATTTTATCACGTTTCAAACCCAAACTTTAAAAGAGCTCGAATTAGAGTCTCTTTCAGAGCATACAT
TAACGGCAGATGATATAAATAAGCTGCCGGATCAAGCTTTTGAAAATAAATTTCTTGTCTTTTTAGATGTGCCATCTTCA
CTTTCTGAATTTAGCGTATGGCTAGAAACGAAAGGTGAACCAGACCGAATATATGCTGTTTTTCATCAGAATTCGGATCA
CTTTTTTACAACATTGCCTACAAGAGAACACTTTAAATGGTTTTATGCATTTTTAGCCAAAAGAAAGACATTTAATTTGA
AAAAAGATGGGAATGAACTGGCTAAATGGAAAGGGTGGTCAAAAGACACGATTGATTTTATGTCACAGGTGTTTTTTGAG
CTCGAATTTGTTACAATAGACGATGGCGTGATTACAATAAATAGTAAACCCGGCAAAAGGGATTTAGAAAGCTCAGAAAC
CTATCAGAAGAAATTTGAACAGGTTGATATCGAAAATCAGCTTGTCTATTCTTCCTATCATGCTTTAAAAAGCTGGTTTA
CACAGCAAACTCGGCAAAAAGCGAGTTTGTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(314-438)

RecJ OB domain (RecJ_OB)

(455-562)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(569-773)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

50.96

99.745

0.508