Detailed information NTG0876447

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: RGQ10_RS10105
• Genome accession: NZ_CP133760
• Coordinates: 2023006..2025384 (-)
• Length: 792 a.a.
• NCBI ID: WP_014418522.1
• Uniprot ID: I2C8C6
• Organism: Bacillus velezensis strain F37
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2018006..2030384

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RGQ10_RS10070 (RGQ10_10070)	-	2019078..2019806 (+)	729	WP_014418519.1	amino acid ABC transporter ATP-binding protein	-
RGQ10_RS10075 (RGQ10_10075)	-	2019827..2020654 (+)	828	WP_007408208.1	transporter substrate-binding domain-containing protein	-
RGQ10_RS10080 (RGQ10_10080)	-	2020711..2021361 (+)	651	WP_014418520.1	amino acid ABC transporter permease	-
RGQ10_RS10085 (RGQ10_10085)	-	2021380..2022036 (+)	657	WP_014418521.1	amino acid ABC transporter permease	-
RGQ10_RS10090 (RGQ10_10090)	-	2022072..2022203 (-)	132	WP_003152741.1	YrzQ family protein	-
RGQ10_RS10095 (RGQ10_10095)	-	2022225..2022416 (-)	192	WP_003152738.1	hypothetical protein	-
RGQ10_RS10100 (RGQ10_10100)	-	2022429..2022887 (-)	459	WP_007408205.1	PRC-barrel domain-containing protein	-
RGQ10_RS10105 (RGQ10_10105)	recD2	2023006..2025384 (-)	2379	WP_014418522.1	ATP-dependent RecD-like DNA helicase	Machinery gene
RGQ10_RS10110 (RGQ10_10110)	-	2025403..2026023 (-)	621	WP_088461189.1	lipopolysaccharide assembly protein LapB	-
RGQ10_RS10115 (RGQ10_10115)	mnmA	2026111..2027226 (-)	1116	WP_012118077.1	tRNA 2-thiouridine(34) synthase MnmA	-
RGQ10_RS10120 (RGQ10_10120)	-	2027263..2028402 (-)	1140	WP_007612815.1	cysteine desulfurase family protein	-
RGQ10_RS10125 (RGQ10_10125)	cymR	2028421..2028837 (-)	417	WP_003152730.1	cysteine metabolism transcriptional regulator CymR	-
RGQ10_RS10130 (RGQ10_10130)	-	2029032..2030300 (+)	1269	WP_014418523.1	replication-associated recombination protein A	-

Sequence

Protein

• Length: 792 a.a.
• Molecular weight: 88412.10 Da
• Isoelectric Point: 4.9490

>NTDB_id=876447 RGQ10_RS10105 WP_014418522.1 2023006..2025384(-) (recD2) [Bacillus velezensis strain F37]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILTDASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDETFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQMKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide

• Length: 2379 bp

>NTDB_id=876447 RGQ10_RS10105 WP_014418522.1 2023006..2025384(-) (recD2) [Bacillus velezensis strain F37]
GTGGATCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCATAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAAGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTACACATTTTATGGAAAAACCGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGGATCATTCACTATTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTCGGAGACGGCGCGCTCAATAAAATTCTGACGGATGCGTCGGTGCTTT
ACGATGTGCCGAAAATGTCGAAAAAGAAAGCGGATAAGCTTGCCGGAGAGCTGCAGCGGCATCAGGGGCTTGAGCAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCAATGAAAATATATCAGGCCTACGAAACGGAAACCCTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGCTGGGCGGAA
AAATGGGTCTGTCAGGAAACCACCCGGAGCGGATCAAAGCGGCAATCTTATATACGATTGAATCGACCTGTCTTTCAGAA
GGCCATACGTACATAGAGACAAAGCAGCTGATTATCAATACGCAAACGCTCCTGAATCAGTCAGCTGAAGGCGGCCGCGT
CACGGAGATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATAGAAGGAGAGCGCTGCTATT
TTCCGTCCTTGTTTTACGCCGAACAAAATGTCGCAAAACGCGTGCTCGACATTGCGGCTCAGACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTCGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTATTGACCGGAGGACCCGGGACGGGGAAAACGACCGTTATTAAAG
GAATCGTGGAATTATACAGTGAACTGCACGGAGTATCGCTTGACCCCGGTGATTATAAAAAAGACGAGACGTTCCCGGTG
CTGCTTGCCGCGCCGACGGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGGCTGCCGGCTGTTACGATTCACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTACAATGAAGATCAGCCGATTGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTCGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTCGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGACCTTTTGGCTTCTGAGGTGCTGCCGACCGTGCGTTT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCGCATGATATGAAAAAAGGACTGCTTCCAAAAG
GGCTGACCGCGCCGACAAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATGAAAGAAGTCGTGGATCAGGTCGTC
TCGAACGCGTTAAAAAAGGGGTATACCTCCAGGGATATTCAAGTGCTTGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAAGACATCATGAATCCGCCGAAAGAAAAAAGGCGGGAGCTGAAATTCGGTGATGTCGTCT
ACAGGACGGGCGATAAAATATTACAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAATGGCGATATCGGCGAAATCGTG
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCGTTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCTTACTGCTGTTCCATTCATAAGTCGCAGGGAAGCGAATTCCCGATTGTCG
TCCTGCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATAACAAGGGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTCAAAAACAATGATGCCACCGTCAGACAGACGTCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATATG
ATGCGAACATCGGAATGGAAGGCATCACGCCGTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix containing domain (HHH_RecD2) (154-244)

• AAA domain (AAA_30) (341-548)

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (589-659)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (676-724)

3D structure

• AlphaFold DB: I2C8C6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	88.622	99.874	0.885