Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   RFN66_RS06850 Genome accession   NZ_CP133705
Coordinates   1308095..1311595 (+) Length   1166 a.a.
NCBI ID   WP_128822816.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain CP47     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1303095..1316595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN66_RS06825 (RFN66_06825) - 1303764..1305131 (-) 1368 WP_025811704.1 cell wall metabolism sensor histidine kinase WalK -
  RFN66_RS06830 (RFN66_06830) - 1305128..1305769 (-) 642 WP_043054576.1 response regulator transcription factor -
  RFN66_RS06835 (RFN66_06835) - 1305883..1306323 (-) 441 WP_020450867.1 ferritin-like domain-containing protein -
  RFN66_RS06840 (RFN66_06840) modA 1306562..1307320 (+) 759 WP_043054577.1 molybdate ABC transporter substrate-binding protein -
  RFN66_RS06845 (RFN66_06845) modB 1307329..1307985 (+) 657 WP_003180426.1 molybdate ABC transporter permease subunit -
  RFN66_RS06850 (RFN66_06850) addB 1308095..1311595 (+) 3501 WP_128822816.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  RFN66_RS06855 (RFN66_06855) addA 1311582..1315274 (+) 3693 WP_205802214.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  RFN66_RS06860 (RFN66_06860) sbcD 1315340..1316521 (+) 1182 WP_039072651.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134594.62 Da        Isoelectric Point: 5.6325

>NTDB_id=875891 RFN66_RS06850 WP_128822816.1 1308095..1311595(+) (addB) [Bacillus paralicheniformis strain CP47]
MGAVFLSGRSGSGKTTFILNEIREKLRDEPLGKPIIFLVPDQMTFLMEYELSKTPDLGGTIRAQVYSFSRLAWRVLQHTG
GMNRPFLTGTGIQMLLRRLIEEHKGEFKVYQNASDKSGFTEQVERMLTEFKRHCLRPESIRDMIEGTGKSEYEDERILSD
KLHDLYILYSKLEENLENQYVQSEDYLTLLAEQIPYADEIRNAAVYVDGFHQFTPQEMNVLEQLMVHAEEITFSLTADQP
FTANSPNELHLFRMTGKAYYDLYQKAKELGLDPSEVRLEETKRHQQHSELRHLERYFDERPAKPYRGRTESLRIMQASNR
RTEIEGIAREIHSLIRQGRFRLRDIAVIARNVEDYKDTIKEVFKDCELPFFIDGKESMQNHPLIELIRSTLDIIKGNWRY
EAVFRCVKTELLFPEGQPKERLREQIDQLENYCIAYGIKGDRWTSKDPFVYRRYSSLDEDFAKTDKEIETENMLNELKGW
IVPPIHRLQKRLKKAETVREMAEAVYLYLEEADVPMKLEQERRLAEESGRIAESRQHEQVWDAVIQLLDEFVEMMGTERI
SFALFQQMIETGLESLKFALIPPALDQVFIGNMDLSRMYGTKCTFLIGVNDGILPARPADDGVLSDDDREWLKRNGAQLA
ATGREQLLDENFLIYMTLSSPSEKLYVSYPIADSEGKTLLPSTVVKRLNELFPDGEEKMLIHEPEQLDDEAQLEFLVNKG
AALSHLAGQLGIWTRQYAISDVWWSTYNFLMNEPDRIFSQNILSSLFFRNKVENLNRHVSRDLYGEHIQGSVSRMETFKA
CPFSHFASHGLKLKERQFFKLEAPDIGQLFHSALKLISDRLHELKLDWRDLTKAQCETLSSDAVERLAPKLQKEILLSSN
RHHYVKQKLQKIIARVSGILSEHAKASGFAPVGIELGFGGKGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSTDWLGMKASPAGVLYFHVHDPMIQASVPLGLDEIEKEIFKKFKMKGL
LLGDQEAVKLMDTTLEQGRSNMISAGLKKDGSLRSDSDVIAEEDFHVLRRHIRRTFQKAGEEITDGKVSIEPYKLKDRTP
CTYCSYRSFCQFDESLEENEYRILKPEKDNVILERLKKEDETDGDF

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=875891 RFN66_RS06850 WP_128822816.1 1308095..1311595(+) (addB) [Bacillus paralicheniformis strain CP47]
GTGGGAGCAGTATTTTTATCAGGCAGATCCGGGAGTGGGAAAACGACCTTTATCCTGAATGAAATCAGAGAAAAACTTCG
CGATGAACCGCTCGGAAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTAGCAAAA
CACCCGATTTGGGCGGAACTATCCGCGCCCAAGTGTACAGTTTTTCAAGGCTTGCCTGGCGAGTGCTCCAGCATACCGGA
GGCATGAATCGGCCGTTTTTGACGGGGACGGGCATTCAGATGCTGCTGCGGAGACTGATTGAAGAGCATAAGGGCGAGTT
TAAAGTCTATCAAAACGCAAGCGATAAAAGCGGATTTACGGAGCAGGTGGAACGGATGCTGACCGAATTTAAACGGCATT
GTCTGCGGCCTGAAAGCATCCGGGACATGATAGAGGGGACGGGCAAATCGGAATACGAAGATGAACGGATCCTTTCCGAT
AAATTGCACGACTTATATATTTTATACAGCAAGCTTGAGGAAAATCTGGAGAATCAATATGTTCAATCTGAGGATTATTT
GACGCTTTTGGCCGAGCAGATTCCGTATGCGGATGAGATTAGAAACGCTGCCGTTTACGTGGACGGATTTCACCAATTTA
CGCCGCAGGAGATGAACGTGCTGGAACAGCTGATGGTACATGCGGAGGAGATCACTTTTTCGCTGACGGCGGATCAGCCG
TTTACGGCCAACAGCCCGAATGAGCTCCATCTGTTCAGAATGACCGGGAAGGCGTACTATGACCTTTACCAGAAGGCGAA
GGAACTTGGCCTTGATCCGTCAGAAGTGCGGCTGGAAGAAACGAAACGGCACCAGCAACATTCGGAGCTCCGGCACCTTG
AACGCTATTTTGACGAGCGTCCTGCAAAACCGTATCGCGGACGGACGGAATCGCTCCGCATCATGCAGGCGTCGAACAGA
AGAACCGAGATTGAAGGAATTGCGCGCGAGATTCATTCTTTAATTCGCCAGGGCCGCTTCAGGCTTAGGGATATCGCCGT
TATTGCCCGGAATGTTGAAGATTATAAAGATACGATAAAAGAAGTTTTTAAAGACTGTGAACTTCCTTTTTTCATAGACG
GCAAGGAATCGATGCAAAATCATCCGCTCATTGAGCTGATCCGTTCGACGCTTGATATCATTAAAGGAAACTGGCGGTAT
GAAGCGGTGTTCCGCTGTGTCAAAACGGAACTTTTATTCCCGGAAGGACAGCCGAAGGAACGGCTGCGCGAACAGATTGA
CCAGCTTGAAAACTACTGTATTGCGTATGGCATTAAAGGAGACAGATGGACAAGCAAAGATCCGTTTGTTTACAGAAGGT
ACTCTTCACTGGATGAGGATTTTGCCAAGACAGACAAAGAGATTGAAACGGAAAATATGCTCAATGAACTAAAGGGCTGG
ATCGTTCCACCGATCCACAGGCTGCAGAAGCGCTTGAAAAAAGCTGAAACCGTCAGGGAAATGGCCGAAGCCGTTTACCT
TTATCTTGAAGAAGCCGATGTTCCGATGAAGCTTGAACAGGAAAGGCGGCTGGCCGAAGAAAGCGGTAGGATTGCAGAGT
CGCGCCAGCATGAGCAAGTATGGGATGCCGTTATTCAACTGTTGGACGAGTTTGTTGAAATGATGGGCACAGAGCGGATA
TCGTTTGCTTTATTTCAGCAAATGATTGAAACCGGCTTGGAGTCTTTGAAATTCGCGCTGATTCCGCCCGCTCTTGATCA
AGTGTTTATCGGCAATATGGACCTTTCAAGGATGTACGGCACGAAATGCACATTTCTCATCGGTGTAAACGACGGTATAC
TTCCGGCGAGGCCTGCGGACGACGGCGTGCTTTCAGACGATGACCGGGAGTGGCTGAAACGAAACGGCGCACAGCTGGCT
GCGACGGGTCGAGAGCAGCTCTTGGATGAGAATTTTCTGATCTACATGACGCTTTCCAGCCCGTCTGAAAAGCTCTATGT
CTCCTATCCGATCGCAGATTCGGAAGGGAAGACGCTGCTTCCTTCAACAGTAGTGAAACGGCTGAATGAGCTGTTCCCGG
ACGGCGAAGAGAAGATGCTGATTCATGAACCCGAGCAGCTGGACGACGAAGCCCAGCTTGAATTTCTCGTGAATAAAGGC
GCCGCCTTGAGCCATTTGGCAGGGCAGCTCGGCATTTGGACGAGGCAGTATGCGATCAGCGATGTATGGTGGAGTACATA
CAACTTTTTAATGAATGAGCCTGACCGCATCTTTTCTCAAAATATCCTGTCCAGCCTGTTTTTCAGAAACAAGGTCGAAA
ACTTGAACCGGCATGTGAGCCGCGATTTATATGGGGAGCACATTCAGGGAAGCGTGTCGCGGATGGAGACGTTCAAAGCC
TGTCCGTTTTCCCATTTTGCTTCGCACGGTTTAAAGCTGAAGGAACGTCAATTTTTCAAGCTTGAAGCTCCGGATATCGG
TCAGCTGTTCCACTCGGCGCTGAAGCTGATTTCAGATCGGCTTCACGAGCTTAAACTGGATTGGCGCGACTTAACGAAAG
CCCAATGCGAGACTTTGTCAAGCGATGCAGTTGAACGGCTCGCGCCAAAGCTGCAAAAAGAGATTTTGCTCAGTTCGAAC
CGTCATCATTACGTGAAGCAGAAGCTGCAAAAAATCATTGCCAGGGTATCGGGCATTTTAAGCGAGCATGCAAAAGCAAG
CGGGTTTGCCCCCGTCGGCATCGAGCTCGGATTCGGGGGGAAAGGTCCGCTTCCTCCAATGCGTTTTACCTTGAAGAACG
GCTGCACAATGGAGCTTGTCGGCCGGATCGACAGAGTGGATAAAGCGGAGAGCTCAAAAGGGCTGCTGCTTAGGATTGTC
GACTATAAATCAAGCGATAAAGGCCTCGATTTGGCCGAGGTGTATTACGGCCTCGCCCTGCAAATGCTGACATATCTCGA
TTTGTCGATCACACATTCAACCGATTGGCTCGGCATGAAGGCATCCCCTGCAGGCGTGCTTTATTTTCATGTGCACGATC
CGATGATCCAGGCTTCGGTGCCGCTCGGCCTTGATGAAATAGAAAAAGAGATTTTCAAAAAGTTCAAGATGAAAGGGCTG
CTCTTGGGAGACCAGGAAGCGGTCAAGCTGATGGATACCACGCTGGAACAAGGCAGGTCGAACATGATCAGCGCAGGCTT
GAAAAAAGACGGCTCGCTCCGTTCGGATTCTGACGTTATTGCAGAAGAGGATTTTCATGTATTAAGGCGCCATATCCGCC
GGACATTTCAGAAGGCCGGCGAGGAGATCACAGACGGTAAAGTGTCGATCGAGCCTTACAAGCTAAAAGACCGGACACCG
TGCACATATTGTTCGTACCGGTCATTCTGCCAGTTTGACGAATCGCTTGAGGAAAACGAATACCGGATATTAAAACCCGA
AAAAGACAACGTGATTTTGGAGCGGCTGAAAAAGGAGGATGAGACAGATGGAGATTTCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

73.82

99.914

0.738