Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   RFB12_RS01370 Genome accession   NZ_CP133588
Coordinates   236593..238950 (-) Length   785 a.a.
NCBI ID   WP_309218404.1    Uniprot ID   -
Organism   Parageobacillus toebii strain NEB857     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 231593..243950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFB12_RS01350 (RFB12_01350) - 232926..233102 (-) 177 WP_015864616.1 hypothetical protein -
  RFB12_RS01355 (RFB12_01355) dtd 233212..233670 (-) 459 WP_285755658.1 D-aminoacyl-tRNA deacylase -
  RFB12_RS01360 (RFB12_01360) - 233685..235883 (-) 2199 WP_285755657.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  RFB12_RS01365 (RFB12_01365) - 236081..236593 (-) 513 WP_015864619.1 adenine phosphoribosyltransferase -
  RFB12_RS01370 (RFB12_01370) recJ 236593..238950 (-) 2358 WP_309218404.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  RFB12_RS01375 (RFB12_01375) - 239035..240123 (-) 1089 WP_309218405.1 IS1182 family transposase -
  RFB12_RS01380 (RFB12_01380) - 240248..240571 (-) 324 Protein_274 transposase -
  RFB12_RS01385 (RFB12_01385) secDF 240690..242942 (-) 2253 WP_089097946.1 protein translocase subunit SecDF -
  RFB12_RS01390 (RFB12_01390) - 243139..243435 (-) 297 WP_062753020.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 88068.34 Da        Isoelectric Point: 5.4834

>NTDB_id=875202 RFB12_RS01370 WP_309218404.1 236593..238950(-) (recJ) [Parageobacillus toebii strain NEB857]
MLIAKTRWDVKRPDENIVTHLVEQSHMDPLIAKLLVHRGVRTVEEANAFLYPLKQPFHDPFLLDGMERAIERIQRAIQHG
EHILVYGDYDADGVSSTTVMVSALKEAGAIVDFYIPNRFTEGYGPNENAFRWAKEQGFSLIITVDNGIAAVKEVALANEL
GMDVIITDHHEVGPALPEAYEIIHPKKPGSTYPFRELAGVGVAFKVAHALLGDVPRHFLDVVAIGTIADLVSLTGENRLL
VAQGLEQLRNTKRIGLRALCKQCGIEAKEINEQTVGFVIAPRMNAVGRLGEATPAVRLLMTEKEEEAQRLAKEMDDLNRE
RQQLVAKIAEEAAQIVNEQFPPSENKVLVIAKEGWNPGVIGIVASRLVEQFYRPTIVLSIDSEKGIAKGSARSIHGFDMF
SSLSTCRDILPHFGGHPMAAGMTLSVDHIDELRQRLNALADDMLTADDFTPITPIDAVCSVSDLTLSVAEQLEKFAPFGV
DNAKPLFLMENVSVETIRRIGADQSHLKAVFAQNGAAIDSVGFGFGYLCDEIAPDAKVSVVGELMVNEWNNLRKPQLMIC
DVAVHECQLFDIRGMRDVRPLIASLPKDKRMLIMFREGTADALGLEAYKEEIYYTATVEEASRLCLDNRYVVLLDMPTSL
EIIKMLLRSSLPARIYALFYQRETHFFRTLPTRDHFKWFYAFLRKKGVFNLAKYGGELARARGWTEETVRFMAKVFLELE
FITQQDGVVSLVPQPAKRDLSESPTYRFKQAQFELENLFLYSTYEQLKRCLFEMKGSQTYEEANV

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=875202 RFB12_RS01370 WP_309218404.1 236593..238950(-) (recJ) [Parageobacillus toebii strain NEB857]
ATGTTAATAGCAAAAACACGTTGGGATGTGAAACGTCCTGACGAAAATATCGTAACGCATTTAGTAGAACAGTCGCATAT
GGATCCGTTGATTGCGAAGCTTCTTGTTCATCGTGGAGTTCGTACGGTAGAAGAGGCGAACGCGTTTTTATATCCGCTGA
AACAACCGTTTCATGATCCGTTTTTGCTAGATGGGATGGAACGGGCAATTGAGCGGATTCAACGGGCGATACAACATGGA
GAGCATATTTTGGTATATGGTGATTATGATGCTGACGGCGTAAGCAGCACGACGGTAATGGTTAGTGCTCTGAAGGAAGC
TGGCGCCATTGTGGATTTTTACATTCCCAATCGATTTACCGAAGGATACGGTCCGAATGAGAATGCATTTCGTTGGGCAA
AGGAGCAAGGATTTTCTCTGATTATCACGGTCGATAACGGCATTGCTGCAGTGAAGGAAGTGGCGCTTGCCAATGAGCTT
GGTATGGATGTCATTATTACAGATCATCATGAAGTGGGACCGGCGTTGCCGGAAGCATATGAAATTATTCATCCGAAAAA
GCCCGGCAGTACATACCCGTTTCGTGAATTAGCAGGCGTCGGAGTAGCATTTAAAGTTGCCCACGCATTGCTTGGGGATG
TGCCGCGTCATTTTTTAGATGTCGTAGCAATTGGAACGATCGCTGATCTCGTTTCCCTTACCGGAGAAAATCGTCTGTTG
GTTGCACAAGGGCTTGAACAGTTGCGAAATACGAAACGTATTGGATTGCGTGCATTGTGTAAACAATGTGGAATAGAAGC
AAAAGAAATTAACGAACAGACGGTTGGGTTCGTTATTGCGCCGCGGATGAATGCAGTTGGCCGGTTGGGAGAAGCAACCC
CTGCTGTCAGATTGTTAATGACGGAGAAAGAAGAGGAAGCACAAAGACTTGCGAAAGAAATGGATGATTTAAATCGGGAG
CGGCAGCAGCTTGTCGCGAAAATAGCGGAGGAAGCGGCACAAATAGTAAATGAACAATTTCCGCCATCCGAAAATAAAGT
GCTTGTGATCGCAAAAGAAGGATGGAATCCAGGGGTCATTGGCATTGTTGCTTCCCGATTGGTTGAACAATTTTACCGTC
CGACGATTGTTTTGAGCATTGATTCCGAGAAAGGAATTGCAAAAGGTTCGGCGCGAAGCATTCATGGGTTTGATATGTTT
TCAAGCTTATCGACATGTCGCGATATTTTGCCACATTTTGGCGGGCATCCAATGGCGGCGGGAATGACATTATCGGTTGA
TCATATTGATGAATTGCGTCAGCGTCTCAACGCACTTGCCGATGATATGTTGACAGCCGATGATTTTACCCCAATTACAC
CGATTGATGCAGTTTGTTCCGTATCTGATTTAACGCTTTCGGTAGCCGAACAGCTTGAAAAGTTTGCTCCGTTTGGTGTA
GATAACGCAAAGCCTCTTTTTTTAATGGAAAATGTATCAGTGGAAACGATCCGACGCATCGGGGCTGACCAGTCGCATTT
AAAGGCTGTTTTCGCCCAAAATGGCGCTGCGATTGATAGCGTCGGGTTCGGCTTTGGTTATTTGTGCGATGAAATCGCTC
CAGATGCGAAAGTGTCCGTGGTTGGCGAGTTAATGGTGAATGAGTGGAACAATCTTCGCAAACCGCAGCTAATGATTTGC
GATGTAGCGGTGCATGAATGTCAATTGTTTGATATTCGCGGTATGCGCGATGTACGGCCACTGATTGCGTCTCTTCCGAA
AGATAAGCGGATGTTGATTATGTTTCGGGAAGGTACGGCAGATGCATTAGGGCTTGAAGCATATAAAGAAGAGATTTACT
ACACGGCCACAGTGGAAGAAGCGTCGCGGCTTTGTTTAGATAACCGGTACGTTGTGCTGTTAGATATGCCAACGTCTTTG
GAAATCATAAAAATGTTGTTACGTTCTAGTTTACCTGCGCGCATTTATGCGTTATTCTATCAAAGGGAGACTCATTTTTT
TCGCACGTTGCCAACGCGTGATCATTTTAAATGGTTTTATGCGTTTTTGAGAAAAAAAGGTGTGTTTAACTTGGCTAAGT
ATGGTGGCGAGTTAGCACGTGCGCGTGGATGGACAGAAGAAACTGTCCGGTTTATGGCAAAAGTATTTCTCGAGCTGGAA
TTTATTACGCAACAGGATGGAGTTGTATCGCTTGTTCCTCAACCGGCTAAACGCGATTTAAGCGAATCGCCGACTTATCG
CTTTAAACAAGCTCAGTTTGAGCTTGAAAATCTATTTTTGTATTCAACATATGAACAATTAAAGCGTTGTCTTTTCGAAA
TGAAAGGTTCGCAAACATACGAGGAGGCAAATGTGTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(302-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-771)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.129

99.745

0.53