Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   RE735_RS07465 Genome accession   NZ_CP133585
Coordinates   1399688..1402021 (+) Length   777 a.a.
NCBI ID   WP_309175159.1    Uniprot ID   -
Organism   Bacillus aerius strain NEB1378     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1394688..1407021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE735_RS07445 (RE735_07445) - 1395699..1395995 (+) 297 WP_159144963.1 post-transcriptional regulator -
  RE735_RS07450 (RE735_07450) secDF 1396037..1398265 (+) 2229 WP_309175156.1 protein translocase subunit SecDF -
  RE735_RS07455 (RE735_07455) - 1398661..1399149 (+) 489 WP_309175157.1 cation:proton antiporter regulatory subunit -
  RE735_RS07460 (RE735_07460) - 1399249..1399605 (+) 357 WP_309175158.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  RE735_RS07465 (RE735_07465) recJ 1399688..1402021 (+) 2334 WP_309175159.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  RE735_RS07470 (RE735_07470) - 1402045..1402557 (+) 513 WP_008342729.1 adenine phosphoribosyltransferase -
  RE735_RS07475 (RE735_07475) - 1402700..1404910 (+) 2211 WP_309175160.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  RE735_RS07480 (RE735_07480) dtd 1404928..1405371 (+) 444 WP_309175161.1 D-aminoacyl-tRNA deacylase -

Sequence

Protein


Download         Length: 777 a.a.        Molecular weight: 87596.49 Da        Isoelectric Point: 4.8610

>NTDB_id=875104 RE735_RS07465 WP_309175159.1 1399688..1402021(+) (recJ) [Bacillus aerius strain NEB1378]
MLLSKMRWEYEHPSEEKVKSLSENLNISVLTASLLVQRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVELLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSRRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDMDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGANQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFVPIQRVDAVCKVDEMTVQAIEEVGLLSPFGMQN
PKPFIMIEDAQLEDIRTIGANQNHIKMSLKDEDKLLDCVGFHQGKLKEELVPGSLISVVGEVSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSALDPAKTMVFCFDEETKRQSELVDIPIHLIDAENVSTFDVKEKYVIFADVPADEHLLKL
LLENQQPARIYTVFQRKEEHFMSSFPSRDQFKWFYGFLFKQGSFPLKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIE
NGVLSIVRDAPKKDLTASSSYAAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2334 bp        

>NTDB_id=875104 RE735_RS07465 WP_309175159.1 1399688..1402021(+) (recJ) [Bacillus aerius strain NEB1378]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCTAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGTGCTGACTGCATCACTTCTTGTACAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCTTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGATTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACACTTAAAGAGCG
GTCTGCGAAGGTAGACTTTTATATTCCAGATCGGTTTAAAGAAGGTTATGGGCCAAACGAACAGGCTTTCCGATATATCA
AAGAGCAGGGAGCATCGCTTGTCATCACTGTGGATACAGGGATCGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATTATCACAGATCACCACGAACCGAGCGAAGAGCTGCCTGAAGCCCTTGCGATTGTACACCCGAAGCA
GCCGGGATGTGAGTATCCATTTAAAGAGCTGGCAGGTGTCGGTGTTGCGTTTAAAGTGGCACATGCACTCCTCGGTAAAC
TCCCAGTAGAATTATTAGATCTTGCTGCAATCGGTACAATTGCGGATCTTGTCCCGCTGCATGATGAAAATAGATGGCTT
GCAAAAAAAGGCCTGATGCAGCTTCGTCAATCTAGGCGGCCTGGGTTAAAAGCGTTATTAAAAGAAGCAGGCGCCACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCACCAAGGCTGAATGCTGTCGGACGAATTGAACAGGCAGATC
CGGCTGTTCATCTTCTCATGACTGAGGATATGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAACTGAACAAAGAG
CGGCAGAAAATTGTGAGTACGATCACAGAAGAAGCCATTCAAATGGTCGAAGAGACAGGGGCTAACCAGTCTGCTATCGT
CGTAGCAAAAGCTGGCTGGAATCCAGGCGTTGTCGGCATTGTTGCCTCAAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGACGAAGAAACGCAGATGGCGAAAGGGTCAGCACGAAGTATTCCTGGCTTTGATTTATTTTTCCAT
TTGAGCAAGTGCCGTGACATCCTGCCGCATTTCGGTGGACACCCGATGGCAGCAGGAATGACACTTCGAGCGGATGATGT
TCCTTTACTTAGGGAAAGATTGAATCAATATGCAAATGAAACATTGACAGAAGAAGATTTTGTGCCAATTCAACGGGTCG
ATGCAGTATGTAAAGTGGACGAAATGACGGTGCAAGCCATTGAAGAAGTGGGGCTGCTGTCCCCGTTTGGCATGCAGAAT
CCTAAGCCGTTCATTATGATTGAAGACGCCCAGCTTGAAGACATCCGAACCATTGGGGCCAATCAAAATCACATTAAAAT
GTCACTAAAAGACGAAGACAAGTTGCTGGATTGTGTCGGTTTTCACCAAGGTAAACTAAAAGAAGAACTCGTCCCAGGGA
GCCTGATTTCTGTCGTTGGCGAGGTCTCGATTAATGAATGGAATAACCGAAAGAAACCGCAGCTCATGTTAAAAGACGCT
AGAGTCGATGAATGGCAGCTGTTTGATTTACGTGGGAAAAAAGATTGGGAAAAGAAAGTATCAGCACTTGATCCAGCAAA
AACGATGGTCTTCTGTTTTGACGAAGAAACAAAAAGGCAATCGGAGCTAGTGGATATTCCAATTCATCTTATTGATGCAG
AAAATGTATCCACGTTTGATGTGAAGGAAAAGTATGTGATCTTTGCCGATGTTCCTGCTGATGAACACTTGCTCAAGCTG
CTTTTAGAAAATCAACAACCAGCAAGAATTTATACGGTGTTTCAGCGGAAAGAAGAACACTTTATGTCCTCATTTCCGAG
CCGTGACCAATTTAAATGGTTTTATGGGTTCTTGTTCAAACAGGGAAGTTTTCCTCTCAAAGAACAAGGCATGGAGCTTG
CCAAACATAGAGGTTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAG
AATGGTGTGCTGTCAATAGTGCGTGATGCCCCTAAAAAAGATTTAACCGCATCATCGTCTTATGCAGCAAAACAACGATT
GATGGAACTCGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCT
CACCTGTGCTTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(314-437)

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

66.795

100

0.668