Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RE395_RS02615 Genome accession   NZ_CP133579
Coordinates   573352..574479 (-) Length   375 a.a.
NCBI ID   WP_014645840.1    Uniprot ID   A0A397M567
Organism   Stenotrophomonas geniculata strain G303     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 568352..579479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE395_RS02595 - 568812..569801 (+) 990 WP_043398812.1 fumarylacetoacetate hydrolase family protein -
  RE395_RS02600 maiA 569816..570499 (+) 684 WP_071306564.1 maleylacetoacetate isomerase -
  RE395_RS02605 - 570533..572029 (-) 1497 WP_171953792.1 UdgX family uracil-DNA binding protein -
  RE395_RS02610 - 572010..573293 (-) 1284 WP_191850603.1 putative DNA modification/repair radical SAM protein -
  RE395_RS02615 pilU 573352..574479 (-) 1128 WP_014645840.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RE395_RS02620 - 574601..574966 (+) 366 WP_010480631.1 DUF4398 domain-containing protein -
  RE395_RS02625 - 574963..575847 (+) 885 WP_010480632.1 hypothetical protein -
  RE395_RS02630 - 576172..576408 (+) 237 WP_005407964.1 YdcH family protein -
  RE395_RS02635 - 576709..578079 (+) 1371 WP_043398797.1 pyridoxal-phosphate dependent enzyme -
  RE395_RS02640 - 578203..579387 (+) 1185 WP_419965546.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41396.73 Da        Isoelectric Point: 6.3741

>NTDB_id=875046 RE395_RS02615 WP_014645840.1 573352..574479(-) (pilU) [Stenotrophomonas geniculata strain G303]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGIIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=875046 RE395_RS02615 WP_014645840.1 573352..574479(-) (pilU) [Stenotrophomonas geniculata strain G303]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCCTGACCACCGGAGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTTTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTTGAGCGCGAGCTGGAGCTCAACATGGCCATTGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGATACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGGAGACGATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCATTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGGCTCGCTGGAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCATCATCGAGCAGGAGGAAGCGCTGCGCGCAGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCACGACCCGTACGCGGATTTCTCGGCCGGCGCGCCGAGCATCACCCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A397M567

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.765

90.667

0.379
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363