Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   RE438_RS22475 Genome accession   NZ_CP133557
Coordinates   4327818..4330157 (-) Length   779 a.a.
NCBI ID   WP_048562233.1    Uniprot ID   -
Organism   Bacillus wiedmannii strain EPS29     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4322818..4335157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE438_RS22455 (RE438_22430) - 4323960..4324136 (-) 177 WP_001242605.1 hypothetical protein -
  RE438_RS22460 (RE438_22435) - 4324406..4324846 (-) 441 WP_048562234.1 D-tyrosyl-tRNA(Tyr) deacylase -
  RE438_RS22465 (RE438_22440) relA 4324864..4327047 (-) 2184 WP_002145623.1 GTP diphosphokinase -
  RE438_RS22470 (RE438_22445) - 4327258..4327770 (-) 513 WP_000346221.1 adenine phosphoribosyltransferase -
  RE438_RS22475 (RE438_22450) recJ 4327818..4330157 (-) 2340 WP_048562233.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  RE438_RS22480 (RE438_22455) - 4330259..4331152 (-) 894 WP_000409734.1 cation diffusion facilitator family transporter -
  RE438_RS22485 (RE438_22460) secDF 4331316..4333580 (-) 2265 WP_048562232.1 protein translocase subunit SecDF -
  RE438_RS22490 (RE438_22465) - 4333851..4334135 (-) 285 WP_048562231.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88231.82 Da        Isoelectric Point: 4.9315

>NTDB_id=874860 RE438_RS22475 WP_048562233.1 4327818..4330157(-) (recJ) [Bacillus wiedmannii strain EPS29]
MLQPKTRWKEKEYNNERVSELANKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSLAPRINAVGRLEDATPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSEEVLNKFSLEDYKEHMMHASEVTHLDDQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKAPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=874860 RE438_RS22475 WP_048562233.1 4327818..4330157(-) (recJ) [Bacillus wiedmannii strain EPS29]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATAATGAGCGAGTGAGTGAATTAGCAAATAAATTACAATT
ATCACCACTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCCTTGCAAGAATT
AGGTGCAGACGTAGAATTTTATATTCCAAACCGTTTTACAGAAGGATATGGACCAAACGAAGAAGCGTTTCGTTGGGCAC
ATAGCGCAGGATTCTCACTAATTATTACTGTCGATACTGGTATCGCAGCAGTGCATGAAGCGAAGGTAGCGAAGGAGCTT
GGAATAGATCTTATTATTACAGATCATCATGAGCCACCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCACATGCACTACTAGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTAGCCGATTTAGTGTCACTTCATGGTGAAAATAGATTGCTA
GTGAAGCGCGGTTTAAAGCATATGCGCATGACGAAAAATATCGGACTAAAGGCGCTATTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCACTCGCACCGCGTATTAATGCAGTTGGACGTTTAGAAGATGCTACGC
CGGCTGTACATCTTTTATTATCAGATGATCCAGAAGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTGGAAAATAATTTCCCGCCAGAAGACAATAAAGT
ACTAGTACTGGCAAAAGAAGGCTGGAATCCAGGTGTAATTGGAATTGTCGCATCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTACTATGCATTGATCCTATAAAAGAAACAGCAAAAGGTTCAGCACGTAGTATTGCAGGATTTGATTTGTTT
GCAAATTTGTCAGATTGTAGAGAGTTATTGCCACACTTCGGAGGGCATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGCTTAAATGAACAAGCAGAGGTAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CTGTTGATGTCTTTTGTAAAGTGGAGGATGTAACACTGGCAGCAATTGAAGACATGCAAAAGTTAGCGCCATTTGGTGTT
GGAAATCCAAAACCGCGTATTGCAGTGAAGGATGCAGAGTTAGAAAGTATTCGTGCAATCGGATCAGATGGCTCTCATTT
GAAAATGGCTCTTCGCGATGGACAAGCGACACTGGATACAATCGGATTTGGTTTTGGTGCGTATGCGAAAGAAATTTCTC
CAGTTGCAAAGGTATCTGTAATAGGGGAAGCATCTATCAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTACAA
GATATTGCTGTAGAGGCGTGGCAATTATTTGATTGGCGTAGTATGCGTAACGTAGAAGCAAATGTAGCAGAACTTCCGAA
AGAAAAAATAACGATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTAACTCATTTAGACGATCAATACATCGTGTTACTTGATTTGCCAAAAGGAACAGATGAATTAAGAGAT
TTATTTAAAGTTGGGTTCCCGTCGCGAATTTATACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGGGAACATTTTAAATGGTACTATTCGTTCTTGAGCCAAAAAGCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.972

99.358

0.526