Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   WJ399_RS26810 Genome accession   NZ_CP149513
Coordinates   5697290..5697715 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 2023CK-01620     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5692290..5702715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ399_RS26795 (WJ399_26795) pilX 5692854..5693441 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  WJ399_RS26800 (WJ399_26800) pilY1 5693453..5696944 (+) 3492 WP_023096128.1 type 4a pilus biogenesis protein PilY1 -
  WJ399_RS26805 (WJ399_26805) pilY2 5696946..5697293 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  WJ399_RS26810 (WJ399_26810) comF 5697290..5697715 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  WJ399_RS26815 (WJ399_26815) ispH 5697762..5698706 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  WJ399_RS26820 (WJ399_26820) fkpB 5698792..5699232 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  WJ399_RS26825 (WJ399_26825) lspA 5699225..5699734 (-) 510 WP_003094728.1 signal peptidase II -
  WJ399_RS26830 (WJ399_26830) ileS 5699727..5702558 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=874850 WJ399_RS26810 WP_003094721.1 5697290..5697715(+) (comF) [Pseudomonas aeruginosa strain 2023CK-01620]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=874850 WJ399_RS26810 WP_003094721.1 5697290..5697715(+) (comF) [Pseudomonas aeruginosa strain 2023CK-01620]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383