Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   RCI28_RS05740 Genome accession   NZ_CP133362
Coordinates   1080375..1083890 (+) Length   1171 a.a.
NCBI ID   WP_016077978.1    Uniprot ID   A0A9X6Z3M6
Organism   Bacillus thuringiensis serovar tenebrionis strain BI 256-82     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1075375..1088890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCI28_RS05705 (RCI28_05680) - 1075832..1076326 (-) 495 WP_016077979.1 DUF6584 family protein -
  RCI28_RS05710 (RCI28_05685) cspA 1076610..1076813 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  RCI28_RS05715 (RCI28_05690) - 1077016..1077219 (-) 204 WP_000356922.1 hypothetical protein -
  RCI28_RS05720 (RCI28_05695) - 1077570..1077755 (-) 186 WP_000391375.1 hypothetical protein -
  RCI28_RS05725 (RCI28_05700) - 1078039..1078620 (+) 582 WP_000444446.1 competence protein ComK -
  RCI28_RS05730 (RCI28_05705) - 1079046..1079639 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  RCI28_RS05735 (RCI28_05710) lepB 1079696..1080259 (+) 564 WP_000751896.1 signal peptidase I -
  RCI28_RS05740 (RCI28_05715) addB 1080375..1083890 (+) 3516 WP_016077978.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  RCI28_RS05745 (RCI28_05720) addA 1083887..1087612 (+) 3726 WP_016077977.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  RCI28_RS05750 (RCI28_05725) - 1087625..1087912 (+) 288 WP_000255718.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  RCI28_RS05755 (RCI28_05730) gerPF 1088050..1088265 (-) 216 WP_001141566.1 spore germination protein GerPF -
  RCI28_RS05760 (RCI28_05735) - 1088308..1088694 (-) 387 WP_000902327.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134201.47 Da        Isoelectric Point: 5.1783

>NTDB_id=873591 RCI28_RS05740 WP_016077978.1 1080375..1083890(+) (addB) [Bacillus thuringiensis serovar tenebrionis strain BI 256-82]
MSLRFVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEGVAREIRKLVADERYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRPPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVSKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASERLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAKKLSTAVSRDLYGDIIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGQAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNILQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=873591 RCI28_RS05740 WP_016077978.1 1080375..1083890(+) (addB) [Bacillus thuringiensis serovar tenebrionis strain BI 256-82]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACGCGAAGTGCAAGAAGAGTTAAA
ACAACGCCCGAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTACTACAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGTGTACATATGTTACTCCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCAGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTGGATGCGCATAGTAGCAGTGCCGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAGCTATTATACGATGATTTTGAACGTGCTTTAATTGGAAAATATTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAATGGTGCGGAAATTTATATAGATGGATTTCATTCAT
TCTCCCCGCAAGAGTTAGAGATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTCGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATCAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCGAGTGTAACGATTAGCACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGTGTTGCTCGTGAAATTCGTAAACTTGTTGCGGATGAAAGGTATCGTTACCGAGATATTGCAGT
GCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGCCCGATGTCACACCACCCATTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGGTGGACTTCAGAGGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATAGGTTACGTGAC
GTTGTAAGACCACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTTTA
CTTATTTTTAGAAGAACTGGATGTTTCGAAGAAGCTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACATTTGTAGAGATGCTTGGTGAAGAGAAA
ATGTCTCTTTCTATGTTTACGGACGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCTAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCACTCCAGGTTATCTGACGTGAAAGCAACGTTTATTATAGGTGTAAATGAGGGGG
TCATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCGGCAGGGATTGAATTA
GCGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCGTCTGAGAGGTTATA
CATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCATTTATTACGAATGACGTAAACGATTTATCACGTTCAGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAATTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTCTTATCAAGTTTATTCTATCGAA
ATCGTGCGAAGAAACTAAGTACAGCAGTAAGTAGAGATTTATATGGAGATATAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGTGCATACGCTCATTTTGCACAGCACGGTTTATCACTTAGAGAGCGTGATATTTTCAAGCTTGATGC
ACCGGACATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGTTATTGCGTGAAAACCGTACTTGGGCGG
ATTTATCCATAAAAGAGTGCGAGCATCTTTCTGTTTTAGTAATAGAAGAAATTGCACCGTTATTACAACGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCGAGCGGATTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGATCACTTCCGCCGATGG
AATTCTCACTACCAAATGGTGTGAAGATGGAAGTAGTCGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAGAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGATTTAACAGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCGAATGCACAGACGTGGATGAAAAAAGGCCAAGCAGCATCACCAGCGGGTG
TATTGTATTTTCATATCCATAATCCAATTGTTGAAATGAAAGGTGACGCATCTGAAGCCGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTATTAGGAGATGCGGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGCCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAGCAAGAGTTTA
ACATACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCGTGCACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAGTCACTTGAAGATAA
TCAATTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.488

100

0.495