Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   RCG25_RS07530 Genome accession   NZ_CP133269
Coordinates   1535453..1537816 (+) Length   787 a.a.
NCBI ID   WP_308083050.1    Uniprot ID   -
Organism   Neobacillus sp. PS2-9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1530453..1542816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG25_RS07515 (RCG25_07515) spoVB 1530965..1532515 (-) 1551 WP_308083047.1 stage V sporulation protein B -
  RCG25_RS07520 (RCG25_07520) - 1532654..1532950 (+) 297 WP_308083048.1 post-transcriptional regulator -
  RCG25_RS07525 (RCG25_07525) secDF 1533091..1535355 (+) 2265 WP_308083049.1 protein translocase subunit SecDF -
  RCG25_RS07530 (RCG25_07530) recJ 1535453..1537816 (+) 2364 WP_308083050.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  RCG25_RS07535 (RCG25_07535) - 1537807..1538319 (+) 513 WP_308083051.1 adenine phosphoribosyltransferase -
  RCG25_RS07540 (RCG25_07540) - 1538542..1540737 (+) 2196 WP_308083052.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  RCG25_RS07545 (RCG25_07545) dtd 1540748..1541191 (+) 444 WP_308083053.1 D-aminoacyl-tRNA deacylase -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 88569.26 Da        Isoelectric Point: 5.1430

>NTDB_id=873135 RCG25_RS07530 WP_308083050.1 1535453..1537816(+) (recJ) [Neobacillus sp. PS2-9]
MLKSKTRWIVRKSDNHLVKQLENELKITPLVASLLVNRGLDTVESARYFLFGKEEFHDPFLLKGMDIAVNRIRGAIEKQE
PILIFGDYDADGVSSTSVLMMTLRDLGANVQFYIPNRFTEGYGPNERAFRHAAESGINLIITVDTGISAVHEASIAKELG
MDLIITDHHEPGPVLPEALAIIHPKLPDSIYPFRELAGVGVAFKLAHALYGEVPEHLFEIAVIGTIADLVSLTEENRLIA
KKGLEKLKVTQNVGLKAIFKLAGVDPTAINEETIGFTLAPRINAVGRLEDADMAVQLILTDDPEEAHQLAVEMDALNKTR
QTIVNEITAEAIEIVEKNYPVDSNKVLVIGKEGWNAGVVGIVASRLVDKYYRPTIILCFDSEKGIAKGSARSIEGFDLFK
NLSTCRDILPHFGGHPMAAGMTLNLDNVADLRERLNSLAEDQLTDEHLIPVTYLDEEVRLEDVHLSSLDEMNLLSPFGMD
NPKPKVLINNVDIASMRKIGSEQNHLKVLVNEDGSNLDGIGFGLGALVDQISPASKISLIGELSINEWNNIRKPQIFIHD
LAVESWQLFDYRGQKRINHIAQTVPSENRKLIVFNKEHLDKINASFLPDTTFIQTKEEAEAFDGHHANIVLVDFPPSMEI
LSDLFKGKQPARIYAYFYKESSDFFSTVPTRDHFKWFYAYLLKKGPLDLNRHGDVIAKHRGWSQETIIFMSKVFSELDFV
TINNGFITLNNQTQKRELTDSRTYQIKQKQYALEKDLLFSSFQELKNWFDQMIHESVETEEAIKEWI

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=873135 RCG25_RS07530 WP_308083050.1 1535453..1537816(+) (recJ) [Neobacillus sp. PS2-9]
ATGTTAAAGTCGAAAACAAGATGGATTGTTCGCAAATCTGATAACCATCTAGTTAAACAACTGGAAAATGAATTAAAGAT
AACACCTCTAGTTGCGTCACTACTTGTCAATCGTGGTTTAGATACCGTAGAATCTGCACGGTATTTTTTATTTGGTAAGG
AAGAATTTCATGATCCTTTTCTCTTAAAAGGAATGGATATCGCGGTTAATAGAATTCGTGGTGCGATTGAAAAACAAGAA
CCTATTCTCATTTTCGGCGACTATGACGCGGATGGTGTAAGCAGCACCTCGGTGTTAATGATGACACTGCGTGACCTTGG
TGCAAATGTACAATTTTATATCCCGAATCGTTTTACAGAGGGATATGGACCGAATGAACGTGCATTCCGTCATGCTGCGG
AATCTGGAATAAACTTAATTATAACTGTGGATACAGGAATATCAGCTGTGCATGAAGCGTCGATAGCAAAAGAGCTTGGT
ATGGACCTTATTATTACGGATCACCATGAGCCGGGTCCTGTTCTACCAGAAGCCCTTGCAATCATTCATCCGAAGCTGCC
TGATAGCATTTATCCTTTCCGGGAACTGGCCGGGGTAGGGGTTGCATTTAAACTTGCCCATGCATTGTATGGTGAAGTGC
CGGAGCATTTATTTGAAATTGCCGTCATCGGTACGATTGCCGACTTAGTATCCTTAACGGAGGAAAACCGCCTCATTGCA
AAAAAGGGCTTAGAAAAGCTGAAAGTAACGCAAAATGTTGGACTTAAAGCTATTTTTAAATTAGCTGGAGTAGACCCTAC
AGCTATTAATGAGGAAACGATAGGTTTTACACTCGCCCCAAGGATTAATGCAGTTGGGAGATTAGAAGATGCAGATATGG
CAGTTCAACTGATTCTGACAGATGATCCTGAAGAAGCGCACCAGCTAGCGGTAGAAATGGATGCGTTGAACAAGACAAGG
CAAACCATCGTAAATGAAATCACTGCTGAAGCGATTGAAATCGTTGAAAAAAATTACCCGGTTGACAGCAATAAAGTCCT
TGTCATTGGGAAGGAAGGCTGGAATGCAGGAGTAGTAGGAATTGTTGCATCCAGACTGGTTGATAAGTATTATCGTCCAA
CCATTATTTTATGCTTTGACTCAGAAAAGGGCATTGCAAAAGGTTCAGCCCGAAGTATAGAGGGTTTTGACCTTTTTAAA
AATTTATCGACATGTAGAGATATTCTGCCTCATTTTGGCGGACATCCAATGGCTGCTGGAATGACTTTAAATCTCGATAA
TGTAGCAGACCTTCGAGAGCGGCTTAATAGCCTTGCAGAAGACCAGCTAACGGATGAGCACCTTATCCCGGTCACCTATC
TAGACGAAGAAGTTCGTCTTGAAGATGTTCATCTATCTTCATTAGATGAAATGAACTTACTTTCTCCTTTTGGTATGGAC
AACCCCAAACCAAAAGTTTTAATAAATAATGTTGATATTGCCAGTATGAGAAAAATTGGTAGTGAGCAAAATCATCTTAA
GGTTTTGGTAAATGAGGATGGTTCCAATCTAGATGGGATTGGCTTTGGACTAGGAGCTTTAGTAGATCAAATCTCTCCTG
CCTCGAAAATTTCATTAATTGGAGAATTATCTATTAATGAATGGAACAATATACGTAAACCACAAATCTTTATCCACGAT
CTCGCAGTTGAATCGTGGCAGTTATTTGACTATCGCGGGCAAAAGCGAATCAACCATATCGCCCAAACGGTACCTTCCGA
AAACAGAAAGCTTATTGTGTTCAATAAGGAGCATCTTGATAAAATAAACGCAAGCTTCCTGCCGGACACCACCTTCATTC
AAACAAAAGAAGAAGCAGAGGCCTTTGACGGACATCATGCAAATATCGTCCTGGTGGACTTCCCACCATCAATGGAAATC
CTATCTGATTTGTTTAAAGGGAAACAACCAGCAAGGATTTATGCCTATTTTTATAAAGAATCCAGTGATTTTTTCAGTAC
TGTACCGACTCGTGATCACTTTAAATGGTTCTATGCATATTTGTTAAAAAAGGGACCATTAGACCTTAATCGTCACGGAG
ATGTCATTGCCAAGCACCGCGGCTGGTCGCAGGAAACAATTATCTTTATGTCAAAGGTGTTTTCTGAACTGGATTTTGTT
ACAATAAACAATGGATTTATCACTTTGAATAATCAAACGCAGAAGCGTGAATTAACTGACTCAAGAACTTATCAAATCAA
ACAGAAACAGTACGCTCTTGAAAAGGATTTACTGTTTTCATCCTTCCAGGAATTAAAAAACTGGTTCGATCAAATGATTC
ATGAGTCAGTTGAGACTGAGGAGGCAATTAAAGAATGGATTTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-225)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(567-769)

DHHA1 domain (DHHA1)

(314-438)

RecJ OB domain (RecJ_OB)

(455-560)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.344

99.873

0.553